LEILA ANTONANGELO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina - Líder
19 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 19
conferenceObject Combining Inhibitor of DNA - Binding Proteins and Angigenic Markers Expression Predict Long Term Survival of Patients with Non-Small Cell Lung Cancer(2013) ANTONANGELO, L.; TUMA, T. S.; VARGAS, F. S.; PARRA, E. R.; TERRA, R. M.; ACENCIO, M.; CAPELOZZI, V. L.Background: Inhibitor of DNA binding (Id) proteins is an emerging promise as biologic marker on oncogenic transformation, cancer progression and tumor angiogenesis, the last, by the regulation of vascular endothelial growth factor (VEGF) expression. Design: We evaluated Ids (1, 2 and 3), VEGF expression and microvessel density (CD34+) in tumor and stromal cells and their impact on survival of 85 patients with surgically excised lung squamous cell carcinoma and adenocarcinoma. Immunohistochemistry and morphometry were used for the quantitation and Kaplan-Meyer survival curves and Cox regression for the statistical analyses. Results: It was found that high Id-1 and VEGF expression and high microvessel density were associated with worse prognosis (Log Rank Test, p<0.001). The Cox model controlled for histological type, age, lymph node stage, Ids, VEGF and microvessel density demonstrated that age, lymph node stage, Id1 and Id3 expression and vascular density were significantly associated with overall survival. A point at the median for Id1, Id3 and vascular density divided patients into 2 groups of different prognosis. Those with higher expression of Id1, Id3 and vascular density had a higher risk of death than those with lower Id-1, Id-3 and microvessel density. Conclusions: Inhibitor of DNA binding (Id) proteins and vascular density are strongly related to prognosis, suggesting that treatment strategies aimed for preventing high Ids synthesis, or local responses to angiogenesis may have impact on NSLC survival.conferenceObject Combining Inhibitor of DNA - Binding Proteins and Angigenic Markers Expression Predict Long Term Survival of Patients with Non-Small Cell Lung Cancer(2013) ANTONANGELO, L.; TUMA, T. S.; VARGAS, F. S.; PARRA, E. R.; TERRA, R. M.; ACENCIO, M.; CAPELOZZI, V. L.Background: Inhibitor of DNA binding (Id) proteins is an emerging promise as biologic marker on oncogenic transformation, cancer progression and tumor angiogenesis, the last, by the regulation of vascular endothelial growth factor (VEGF) expression. Design: We evaluated Ids (1, 2 and 3), VEGF expression and microvessel density (CD34+) in tumor and stromal cells and their impact on survival of 85 patients with surgically excised lung squamous cell carcinoma and adenocarcinoma. Immunohistochemistry and morphometry were used for the quantitation and Kaplan-Meyer survival curves and Cox regression for the statistical analyses. Results: It was found that high Id-1 and VEGF expression and high microvessel density were associated with worse prognosis (Log Rank Test, p<0.001). The Cox model controlled for histological type, age, lymph node stage, Ids, VEGF and microvessel density demonstrated that age, lymph node stage, Id1 and Id3 expression and vascular density were significantly associated with overall survival. A point at the median for Id1, Id3 and vascular density divided patients into 2 groups of different prognosis. Those with higher expression of Id1, Id3 and vascular density had a higher risk of death than those with lower Id-1, Id-3 and microvessel density. Conclusions: Inhibitor of DNA binding (Id) proteins and vascular density are strongly related to prognosis, suggesting that treatment strategies aimed for preventing high Ids synthesis, or local responses to angiogenesis may have impact on NSLC survival.conferenceObject Evaluation of a biomarker panel for the diagnosis of cavity effusions(2018) ANTONANGELO, L.; FARIA, C. Silverio; ASCENCIO, M. M. P.; ROSOLEN, D. Cristina Batista; DOI, D.; FARIA, D. KanaanconferenceObject Performance of the UroVysion (R) FISH assay for the diagnosis of malignant effusions using two cutoff strategies(2018) ANTONANGELO, L.; FARIA, D. Kanaan; FARIA, C.; ROSOLEN, D. Cristina BatistaconferenceObject Genomic profile of primary non-small cell lung cancer and matched mediastinal lymph nodes by next-generation sequencing: a pilot study(2022) ANTONANGELO, L.; FARIA, C. S.; TERRA, R. M.; NASCIMENTO, E. C. T. do; MELLO, E. S. de; MANGONE, F. R. R.; NAGAI, M. A.; AGATI, M. E. M.; CAPELOZZI, V. L.conferenceObject Usefulness of type V collagen and alpha 2 beta 1-integrin in the cytological diagnosis of pleural liquid biopsy(2019) ANTONANGELO, L.; TEODORO, W. Rosolia; FARIA, C. Silverio; QUEIROZ, Z. A.; SILVEIRA, L. Ramos da; VELOSA, A. P.; CAPELOZZI, V.conferenceObject Multiplex Ligation Probe-dependent Amplification (MLPA) as an ancillary method for the diagnosis of malignant pleural effusion(2012) PARRA, E.; ROSOLEN, D.; KULIKOWSKI, L.; DUTRA, R.; CAPELOZZI, V. L.; VARGAS, F.; ACENCIO, M.; ANTONANGELO, L.Objective: A definitive diagnosis provided by the finding of malignant cells in pleural fluid (PF) can be established in around 50 % of patients with pleural malignancy. However, underdiagnosis risk in cytological suspicious cases is high, which makes the cytological diagnosis quite limited. This is an important clinical problem, especially if we consider that some patients, in bad clinical conditions, can not be submitted to a guided thoracoscopic biopsy. Method: Using multiplex ligation probe-dependent amplification (P315-MRC-Holland) we have studied sequence variations of EGFR gene and amplifications/deletions of chromosomal regions frequently associated to tumors (ATG4B, PAHs, PROS, NSD1, and CDGIF genes). Results: Forty-three malignant PF samples from patients with different cancers were evaluated, even in those cases with scarce pellet cells. Four benign pleural effusions were used as control. Gene sequence changes were observed in 13 (30.2 %) cases, while others copy number abnormalities were found in 19 (44.2 %). Conclusion: The findings suggest that MLPA could be considered an alternative tool to detect molecular genetic changes in malignant pleural effusions, since this technique is relatively low expensive and not time consuming. Our next challenge is to find the best combination of probes capable to recognize malignant cells of any origin in fresh samples of PF.conferenceObject Elastic-Collagen Profile in Emphysematous Airspaces from Different Chronic Fibrosing Lung Disorders(2013) MARCAL, L. J.; ANTONANGELO, L.; PARRA, E. R.; VARGAS, F. S.; TEODORO, W. R.; NASCIMENTO, E. C. T.; CAPELOZZI, V. L.Background: Parenchyma elastic and collagen components of chronic fibrosing lung disorders has been exhaustively investigated, but little attention has been aimed at the emphysematous airspaces present in bullous disease, smoking-related interstitial disease and usual interstitial pneumonia. The aim of this study was to evaluate whether elastic deposition accompanies collagen deposition in the repairing process of emphysematous airspaces in chronic fibrosing lung injuries. Design: The elastic and collagen fibers distribution were evaluated in emphysematous airspaces of bullous type I and II disease, smoking-related interstitial fibrosis and of usual interstitial pneumonia (UIP). Lung specimens obtained by surgical lung biopsy or by bullectomy divided the patients into four groups: 1) type I bullous disease (SPT-I; n=7); 2) type II bullous disease (SPT-II; n=12); 3) smoking-related interstitial pneumonia (SRIP; n=5) and 4) usual interstitial pneumonia/idiopathic pulmonary fibrosis (UIP/IPF; n=5). Collagen and elastic fibers were identified respectively by Picrosirius-polarization and Weigert's resorcin-fucsin staining and quantified by image analysis.The results were expressed in percentual area. Results: The proportion of collagen fibers was two times higher when compared with the elastic component in the four groups. In addition, a lower percentage of elastic and collagen fibers were observed in SRIP when compared with UIP/IPF, SPT-I and SPT-II. Conclusions: A smaller amount of elastic and collagen fibers accompanies the remodeling of the emphysematous airspaces in smoking-related interstitial pneumonia when compared to the other fibrosing diseases, suggesting a different remodeling spectrum in chronic fibrosing lung diseases.conferenceObject Detection of aneuploidy by flow cytometry and fluorescence in situ hybridization in the diagnosis of malignant pleural effusion(2014) ROSOLEN, D.; DUTRA, A. R. N.; BOTTURA, G.; SILVA, V. M. da; CAPELOZZI, V. L.; VARGAS, F. S.; ANTONANGELO, L.conferenceObject Efficacy of the UroVysion kit for the diagnosis of malignant pleural effusion(2016) ANTONANGELO, L.; DUTRA, A. R. N.; NETO, E. Terreri; MORENA, L. C.; CAPELOZZI, V. L.; TERRA, R. M.; ROSOLEN, D. C. B.