SERGIO CARLOS NAHAS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Gastroenterologia, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina - Médico
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina - Médico
15 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 15
conferenceObject Magnifying Chromoendoscopy Is Decisive to Define Management of Colorectal Neoplastic Lesions(2015) KAWAGUTI, Fabio S.; PENNACCHI, Caterina; MARTINS, Bruno C.; RETES, Felipe A.; MARQUES, Carlos Frederico S.; NAHAS, Caio Sergio R.; SAFATLE-RIBEIRO, Adriana V.; RIBEIRO, Ulysses; NAHAS, Sergio C.; MALUF-FILHO, FauzeconferenceObject EFFICACY OF TRIDIMENSIONAL ENDORECTAL ULTRASOUND IN COMPARISON TO HISTOPATHOLOGY FOR EVALUATION EXTRA PERITONEAL RECTAL NEOPLASMS.(2015) PINTO, R.; CORREA NETO, I.; NAHAS, S.; NAHAS, C.; MARQUES, C.; RIBEIRO JUNIOR, U.; BUSTAMANTE-LOPEZ, L.; SOARES, D.; CECCONELLO, I.- Desmoid tumors: clinical features and outcome of an unpredictable and challenging manifestation of familial adenomatous polyposis(2015) CAMPOS, Fabio Guilherme; MARTINEZ, Carlos Augusto Real; NOVAES, Marleny; NAHAS, Sergio Carlos; CECCONELLO, IvanBackground/Aims Desmoid tumors (DTs) are rare, locally invasive neoplasms that may affect 10-25 % of familial adenomatous polyposis (FAP) patients. Our aim was to evaluate incidence and clinical presentation among our patients, the potential impact on FAP outcome and to discuss treatment. Materials and methods Charts from 133 FAP (1977-2013) were reviewed. Patients with DTs were separated to retrieve demographic, clinical and management data. Follow-up was focused on disease evolution causing complications or death. Results 19 (14.3 %) DTs were diagnosed, either after previous trauma (16) or during FAP surgery (3). This group comprised 8 men (42.1 %) and 11 women (57.9 %) with an average age of 32.9 years. Intervals from surgical trauma to DTs ranged from 7 to 60 months. ECMs were detected in 12 (63.1 %) patients. DTs were located in the abdominal wall (8), abdominal cavity (8), abdominal wall and cavity (2) and left arm (1). Five patients (26.3 %) referred family history of DTs. Patients presented severe complications such as small bowel obstruction (4) and hydronephrosis (2), being directly responsible for death in three patients. Conclusions (1) DTs developed in 14.3 % of FAP, mostly after surgical trauma; (2) 30 % caused severe morbidity; (3) identification of clinical risk factors may help surgeons to develop screening and therapeutic decisions.
conferenceObject OBSERVATION VERSUS SURGICAL RESECTION IN PATIENTS WITH RECTAL CANCER WHO ACHIEVED COMPLETE CLINICAL RESPONSE AFTER NEOADJUVANT CHEMORADIOTHERAPY: PRELIMINARY RESULTS OF A RANDOMIZED TRIAL (NCT02052921).(2015) NAHAS, S.; NAHAS, C.; RIBEIRO JUNIOR, U.; MARQUES, C. Sparapan; COTTI, G. C.; IMPERIALE, A.; ORTEGA, C.; AZAMBUJA, R.; CHEN, A.; HOFF, P.; CECCONELLO, I.- Gastrointestinal stromal tumor of the rectum treated with neoadjuvant Imatinib followed by transanal endoscopic microsurgery(2015) NAHAS, Caio Sergio Rizkallah; NAHAS, Sergio Carlos; MARQUES, Carlos Frederico Sparapan; SCHMERLING, Rafael; BUSTAMANTE-LOPEZ, Leonardo Alfonso; RIBEIRO JUNIOR, Ulysses; CECCONELLO, Ivan
- Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity(2015) PEREIRA, Marina A.; DIAS, Andre R.; FARAJ, Sheila F.; CIRQUEIRA, Cinthya dos S.; TOMITAO, Michele T.; NAHAS, Sergio Carlos; RIBEIRO JR., Ulysses; MELLO, Evandro S. deAims: To compare Carnoy's solution (CS) and 10% neutral buffered formalin solution (NBF) as tissue fixatives in colorectal cancer specimens. Methods and results: Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF. Groups 1 and 2 were analysed for amount, quality and integrity of DNA. Morphological analysis, including some of the usual special stains and polymerase chain reaction (PCR), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (IHC) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA. PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested. Conclusions: Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.
- Proficiency of DNA repair genes and microsatellite instability in operated colorectal cancer patients with clinical suspicion of lynch syndrome(2015) FREITAS, Isabella Nicacio de; CAMPOS, Fabio Guilherme Caserta Maryssael de; ALVES, Venancio Avancini Ferreira; CAVALCANTE, Juliana Magalhaes; CARRARO, Dirce; COUDRY, Renata de Almeida; DINIZ, Marcio Augusto; NAHAS, Sergio Carlos; RIBEIRO JR., UlyssesBackground: Lynch syndrome (LS) diagnosis is underestimated, and most of the patients remain undetected after colorectal resections. The study aims to assess the frequency of LS in patients undergoing surgical treatment for colorectal cancer (CRC). Methods: A total of 458 CRC patients were operated from January 2005 to December 2008. Positive CRC family history (FH) was present in 118 (25.8%) patients. Histologic sections were reviewed for microsatellite instability (MSI) criteria (Bethesda guidelines), immunohistochemical (IHC) analysis for MLH1, MSH2, MSH6, PMS2 proteins, through the avidin-biotin-peroxidase complex, MSI (BAT-25, BAT-26, NR-21, NR-24 and MONO-27) and BRAF somatic mutation. Results: Of the 118 patients with FH, 61 (51.69%) met at least one of the revised Bethesda criteria. IHC was abnormal in 8 (13.1%) and MSI in 12 patients (20%). BRAF was negative in all cases. MSI histopathological included: intratumoral lymphocytes (47.5%), expansive tumors (29.5%) mucinous component (27.8%) and Crohn's like reaction in (14.7%). There was an association between the revised Bethesda criteria with: sex, mucinous histology and Crohn's like reaction; MSI and IHC with PMS2 and MLH1. Revised Bethesda criteria 4 had 10.6 increased chances to display positive MSI. We have proposed a score to contribute as a practical tool in the diagnosis of LS. Conclusions: The frequence of LS in resected CRC patients was 2.6%. The criterion 4 Revised Bethesda was associated more strongly with the presence of MSI.
- Endoscopic radial incision and cutting procedure for a colorectal anastomotic stricture(2015) KAWAGUTI, Fabio Shiguehissa; MARTINS, Bruno da Costa; NAHAS, Caio Sergio Rizkallah; MARQUES, Carlos Frederico Sparapan; RIBEIRO, Ulysses; NAHAS, Sergio Carlos; MALUF-FILHO, Fauze
conferenceObject ENDOSCOPIC ASSISTED COLOSTOMY WITH PERCUTANEOUS COLOPEXY: AN EXPERIMENTAL STUDY.(2015) BUSTAMANTE-LOPEZ, L.; SULBARAN, M.; SAKAI, P.; MOURA, E.; NAHAS, C.; MARQUES, C.; SAKAI, C.; CECCONELLO, I.; PINTO, R.; NAHAS, S.- Functional outcome of autologous anorectal transplantation in an experimental model(2015) SEID, V. E.; GALVAO, F. H. F.; VAIDYA, A.; WAISBERG, D. R.; CRUZ JR., R. J.; CHAIB, E.; NAHAS, S. C.; ARAUJO, S. E. A.; D'ALBUQUERQUE, L. A. C.; ARAKI, J.Background: Although anorectal transplantation is a challenging procedure, it is a promising option for patients who have completely lost anorectal function or in whom it failed to develop, as in congenital malformations. The paucity of animal models with which to test functional outcomes was addressed in this study of anorectal manometry in rats. Methods: Wistar rats were assigned randomly to four groups: orthotopic anorectal transplantation, heterotopic transplantation, sham operation, or normal control. Bodyweight and anal pressure were measured immediately before and after operation, and on postoperative days 7 and 14. ANOVA and Tukey's test were used to compare results for bodyweight, anal manometry and length of procedure. Results: Immediately after the procedure, mean(s.d.) anal pressure in the orthotopic group (n=13) dropped from 31.4(13.1) to 1.6(13.1) cmH(2)O (P < 0.001 versus both sham operation (n=13) and normal control (n=15)), with partial recovery on postoperative day 7 (14.9(13.9) cmH(2)O) (P=0.009 versus normal control) and complete recovery on day 14 (23.7(12.2) cmH(2)O). Heterotopic rats (n=14) demonstrated partial functional recovery: mean(s. d.) anal pressure was 26.9(10.9) cmH(2)O before operation and 8.6(6.8) cmH(2)O on postoperative day 14 (P < 0.001 versus both sham and normal control). Conclusion: Orthotopic anorectal transplantation may result in better functional outcomes than heterotopic procedures.