ALVINA CLARA FELIX
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Enhanced detection of viral diversity using partial and near full-length genomes of human immunodeficiency virus Type 1 provirus deep sequencing data from recently infected donors at four blood centers in Brazil(2015) PESSOA, Rodrigo; WATANABE, Jaqueline Tomoko; CALABRIA, Paula; ALENCAR, Cecilia Salete; LOUREIRO, Paula; LOPES, Maria Esther; PROETTI, Anna Barbara; FELIX, Alvina Clara; SABINO, Ester C.; BUSCH, Michael P.; SANABANI, Sabri S.BackgroundHere, we report application of high-throughput near full-length genome (NFLG) and partial human immunodeficiency virus Type 1 (HIV-1) proviral genome deep sequencing to characterize HIV in recently infected blood donors at four major blood centers in Brazil. Study Design and MethodsFrom 2007 to 2011, a total of 341 HIV+ blood donors from four blood centers were recruited to participate in a case-control study to identify HIV risk factors and motivations to donate. Forty-seven (17 from SAo Paulo, eight from Minas Gerais, 11 from Pernambuco, and 11 from Rio de Janeiro) were classified as recently infected based on testing by less-sensitive enzyme immunoassays. Five overlapping amplicons spanning the HIV genome were polymerase chain reaction amplified from peripheral blood mononuclear cells. The amplicons were molecularly barcoded, pooled, and sequenced by a paired-end protocol (Illumina). ResultsOf the 47 recently infected donor samples studied, 39 (82.9%) NFLGs and six (12.7%) partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Subtype B was the only nonrecombinant virus identified in this study and accounted for 62.2% (28/45) of samples. The remaining 37.8% (17/45) of samples showed various patterns of subtype discordance in different regions of HIV-1 genomes, indicating two to four circulating recombinant subtypes derived from Clades B, F, and C. Fourteen samples (31.1%) from this study harbored drug resistance mutations, indicating higher rate of drug resistance among Brazilian blood donors. ConclusionOur findings revealed a high proportion of HIV-1 recombinants among recently infected blood donors in Brazil, which has implications for future blood screening, diagnosis, therapy, and vaccine development.
- Real-time symptomatic case of transfusion-transmitted dengue(2015) LEVI, Jose Eduardo; NISHIYA, Anna; FELIX, Alvina Clara; SALLES, Nanci Alves; SAMPAIO, Luciana Ribeiro; HANGAI, Fatima; SABINO, Ester Cerdeira; MENDRONE JR., AlfredoBackgroundDengue virus transmission by blood transfusion is a rarely reported event. Case ReportDuring a dengue outbreak in SAo Paulo city, a regular plateletpheresis donor informed the blood bank of being diagnosed a few days after donation. The recipient was hospitalized and displayed symptoms and laboratory evidence of dengue after transfusion. ResultsThe donor was immunoglobulin (Ig)G, IgM, and polymerase chain reaction nonreactive on the index sample, seroconverting 20 days later. The platelet units were transfused into two patients. One of them developed fever 3 days after transfusion, with high viral load. His pretransfusion sample was negative for IgG, IgM, and dengue RNA, while the second recipient did not show any symptoms nor laboratory evidence of dengue infection. ConclusionsThis case brings additional evidence that dengue is indeed transmissible by blood transfusion and clinical manifestations, although rare, do occur.
conferenceObject DENGUE COHORT STUDY, ARARAQUARA, BRAZIL, 2015, BASELINE SEROPREVALENCE(2015) LUNA, Expedito J.; FIGUEIREDO, Gerusa M.; LEVI, Jose E.; FIGUEIREDO, Walter M.; COSTA, Angela A.; CAMPOS, Sergio R.; FELIX, Alvina C.; SOUZA, Ana C.; SOUZA, Nathalia C.; CARDOSO, Maria R.; PANNUTI, Claudio S.