ALVINA CLARA FELIX
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Data on dengue incidence in South-eastern Brazil, 2014-2018(2020) LUNA, Expedito; FIGUEIREDO, Gerusa; LEVI, Jose; CAMPOS, Sergio; FELIX, Alvina; SOUZA, Nathalia; FIGUEIREDO, Walter; COSTA, Angela; CARDOSO, Maria; PANNUTI, ClaudioData from the routine surveillance systems have been extensively used to estimate the incidence of dengue. However, routine surveillance data frequently underestimate the diseases' incidence. Underreporting of dengue cases is related to the varying spectrum of its clinical presentation, with a large proportion of mild and asymptomatic infections, to its unspecific signs and symptoms, to the limitations of access to health care, and to the performance of the surveillance system itself [1-3]. In order to obtain accurate figures on dengue incidence, a cohort of children and adolescents was set up and followed during four years. The incidence of reported cases was used as a reference for the sample size calculation, which was stratified by age groups. A two-stage procedure was used to select the participants: census tracts were randomly selected, and within each one, a pre-determined number of children of each age group was randomly selected. The parents or legal guardians of the participating children and adolescents provided a written informed consent. In the first home visit, they responded to a questionnaire containing data on socio-demographic characteristics, housing, access to water, sewage, and garbage collection. Also, during the first visit a blood sample of the participating child/adolescent was collected for dengue baseline serology. Beginning in the week after the enrolment, the parent or legal guardian that was designated in the first visit received weekly phone calls for fever surveillance. If the child/adolescent had fever during the week, a nurse was dispatched to the family's home to collect more detailed data on the fever episode and collect a blood sample for dengue diagnosis (IgG, IgM, NS1 and PCR). If the dengue diagnosis was confirmed, a medical appointment was scheduled, and another blood sample for confirmatory tests was collected. It was also agreed that in every anniversary of their participation, they would receive another visit for a blood collection for dengue serology, regardless if they had a fever episode or a confirmed dengue diagnosis during the previous year. This article contains the description of the cohort's dataset. It is associated with the article published in Acta Tropica, under the title ""A cohort study to assess the incidence of dengue, Brazil, 2014-2018"" [4]. The associated article focused on the seroprevalence and incidence of dengue, and explored some associations between both outcomes and some explanatory variables. (C) 2020 The Authors.
- A cohort study to assess the incidence of dengue, Brazil, 2014-2018(2020) LUNA, Expedito J. A.; FIGUEIREDO, Gerusa M.; LEVI, Jose E.; CAMPOS, Sergio R. S. L. C.; FELIX, Alvina Clara; SOUZA, Nathalia Santiago E; FIGUEIREDO, Walter M.; COSTA, Angela A.; CARDOSO, Maria R. A.; PANNUTI, Claudio S.The present cohort study was set up with the aim of determining the incidence of dengue among children and adolescents, from 2 to 16 years of age, living in Araraquara, South-Eastern Brazil, a city classified as a mid-level endemicity location for dengue. Enrollment took place from September 2014 to March 2015. Baseline socio-demographic data were collected, and a blood sample from the participant was drawn, for dengue serology. Families were contacted weekly for fever surveillance. If the child developed fever, a nurse visited the household to collect a blood sample. PCR, NS1 and IgM were used for dengue diagnosis. Parents or legal guardians of participating children provided a written informed consent. 3,514 children and adolescents were enrolled in the cohort. Dengue baseline seroprevalence was 12.2% (95%CI: 11.1 - 13.3). The incidence density of symptomatic dengue was 8.94 per 100 person/years in the first year of follow-up, 0.58 in the second, and 0.19 in the fourth. No cases were confirmed in the third year. Incidence was associated with age, sex, baseline seroprevalence and with living in a house as opposed to an apartment. This study provides relevant information on the epidemiology of dengue in mid-level transmission settings that may be useful to policymakers in the evaluation of control strategies.
- High proportion of Guillain-Barre syndrome associated with chikungunya in Northeast Brazil(2020) MATOS, Aline de Moura Brasil; CARVALHO, Fernanda Martins Maia; MALTA, Danielle Lima; RODRIGUES, Cleonisio Leite; FELIX, Alvina Clara; PANNUTI, Claudio Sergio; LIMA, Amanda Dias da Rocha; ESPOSITO, Danilo Lucas Alves; SANTOS, Leonilda Maria Barbosa dos; GLEHN, Felipe von; COLARES, Jeova Keny Baima; FONSECA, Benedito Antonio Lopes da; OLIVEIRA, Augusto Cesar Penalva de; ROMANO, Camila MaltaFrom 2013 to 2015, sanitary authorities reported an increased incidence of Guillain-Barre syndrome (GBS) associated with Zika virus (ZIKV) in French Polynesia, Caribbean, and Brazil.(1-3) After the end of ZIKV epidemics, GBS cases where still above the usual limits in countries where the arrival of chikungunya virus (CHIKV) was also a concern.(3) Here, we report the findings from Hospital Geral de Fortaleza (HGF), a neuroinvasive arboviral disease vigilance center in Ceara State, Northeast Brazil.
- First report of Aedes albopictus infected by Dengue and Zika virus in a rural outbreak in Brazil(2020) REZENDE, Helder Ricas; ROMANO, Camila Malta; CLARO, Ingra Morales; CALEIRO, Giovana Santos; SABINO, Ester Cerdeira; FELIX, Alvina Clara; BISSOLI, Jefferson; HILL, Sarah; FARIA, Nuno Rodrigues; SILVA, Theresa Cristina Cardoso da; SANTOS, Ana Paula Brioschi; CERUTTI JUNIOR, Crispim; VICENTE, Creuza RachelIn Brazil, Dengue (DENV) and Zika (ZIKV) viruses are reported as being transmitted exclusively by Aedes aegypti in urban settings. This study established the vectors and viruses involved in an arbovirus outbreak that occurred in 2019 in a rural area of Espi ' rito Santo state, Brazil. Mosquitoes collected were morphologically identified, sorted in samples, and submitted to molecular analysis for arboviruses detection. Phylogenetic reconstruction was performed for the viral sequence obtained. All 393 mosquitoes were identified as Aedes albopictus. DENV-1 genotype V was present in one sample and another sample was positive for ZIKV. The DENV-1 clustered with viruses that have circulated in previous years in large urban centers of different regions in Brazil. This is the first report of A. albopictus infected by DENV and ZIKV during an outbreak in a rural area in Brazil, indicating its involvement in arboviral transmission. The DENV-1 strain found in the A. albopictus was not new in Brazil, being involved previously in epidemics related to A. aegypti, suggesting the potential to A. albopictus in transmitting viruses already circulating in the Brazilian population. This finding also indicates the possibility of these viruses to disperse across urban and rural settings, imposing additional challenges for the control of the diseases.
- Specificity of NS1-based immunochromatographic tests for dengue virus with regard to the Zika virus protein(2020) ANDREATA-SANTOS, Robert; PEREIRA, Samuel Santos; PEREIRA, Lennon Ramos; FELIX, Alvina Clara; ROMANO, Camila Malta; FERREIRA, Luis Carlos de SouzaObjectives: This study was performed to determine whether Dengue virus (DENV) immunochromatographic tests can detect and differentiate nonstructural protein 1 (NS1) from each of the four DENV serotypes and do not cross-react with the Zika virus (ZIKV) NS1 protein. Methods: We compared the specificity of six NS1-based DENV immunochromatographic tests (point of care) in the detection of NS1 proteins from each of the four DENV serotypes and ZIKV. The tests were performed with NS1 proteins produced in mammalian cells. Cross-reactivity was confirmed with a purified recombinant ZIKV NS1 protein and DENV+ or ZIKV(+) human serum samples. Results: Cross-reaction was observed in 2 out of the 6 evaluated tests using cell culture supernatants containing NS1 protein of each tested virus. Cross-reactivity with ZIKV was confirmed with purified recombinant ZIKV NS1 produced in Escherichia coli. Further analyses with serum samples collected from DENV+ or ZIKV(+) patients confirmed the cross-reactivity with ZIKV protein in 2 tests. Conclusions: The detection of the NS1 protein is the basis for several commercially available serological DENV diagnostic tests. The present results emphasize the relevance of testing specificity of presently available NS1-based DENV serological tests and the need of adjustments of tests that cross-react with the ZIKV protein. Our results are particularly relevant for regions where both viruses are endemically found, as in the case of Brazil. (c) 2020 The Authors.
- Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019(2020) JESUS, Jaqueline Goes de; DUTRA, Karina Rocha; SALES, Flavia Cristina da Silva; CLARO, Ingra Morales; TERZIAN, Ana Carolina; CANDIDO, Darlan da Silva; HILL, Sarah C.; THEZE, Julien; TORRES, Celeste; D'AGOSTINI, Tatiana Lang; FELIX, Alvina Clara; REIS, Andreia F. Negri; ALCANTAR, Luiz Carlos Junior; ABREU, Andre L. de; CRODA, Julio H. R.; OLIVEIRA, Wanderson K. de; FILIPIS, Ana Maria Bispo de; CAMIS, Maria do Carmo Rodrigues dos Santos; ROMANO, Camila Malta; LOMAN, Nick J.; PYBUS, Oliver G.; SABINO, Ester Cerdeira; NOGUEIRA, Mauricio L.; FARIA, Nuno RodriguesBACKGROUND Despite efforts to mitigate the impact of dengue virus (DENV) epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. Sao Paulo State in the southeast of Brazil has reported nearly half of all DENV infections in the country. OBJECTIVES To understand the origin and dynamics of the 2019 DENV outbreak. METHODS Here using portable nanopore sequencing we generated20 new DENV genome sequences from viremic patients with suspected dengue infection residing in two of the most-affected municipalities of Sao Paulo State, Araraquara and Sao Jose do Rio Preto. We conducted a comprehensive phylogenetic analysis with 1,630 global DENV strains to better understand the evolutionary history of the DENV lineages that currently circulate in the region. FINDINGS The new outbreak strains were classified as DENV2 genotype III (American/Asian genotype). Our analysis shows that the 2019 outbreak is the result of a novel DENV lineage that was recently introduced to Brazil from the Caribbean region. Dating phylogeographic analysis suggests that DENV2-III BR-4 was introduced to Brazil in or around early 2014, possibly from the Caribbean region. MAIN CONCLUSIONS Our study describes the early detection of a newly introduced and rapidly-expanding DENV2 virus lineage in Brazil.
- Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics(2020) RIBEIRO, Marizelia R. C.; KHOURI, Ricardo; SOUSA, Patricia S.; BRANCO, Maria R. F. C.; BATISTA, Rosangela F. L.; COSTA, Elaine P. F.; ALVES, Maria T. S. S. B.; AMARAL, Glaucio A.; BORGES, Marcella C. R.; TAKAHASI, Eliana H. M.; GOMES, Lillian N.; MENDES, Ana K. T.; CAVALCANTE, Tamires B.; COSTA, Luciana C.; FELIX, Alvina C.; SOUZA, Nathalia C. S.; SILVA, Antonio A. M.The short duration of viremia, low blood viral load, inaccessibility to timely specific diagnostic tests, and cross-reactions with other flaviviruses have hindered laboratory confirmation of Congenital Zika Syndrome (CZS). This study analyzes the positivity of the plaque reduction neutralization test (PRNT) in children with clinical or imaging characteristics of CZS and its association with laboratory, clinical, and imaging characteristics. The 94 clinical cases of CZS submitted to the ZIKV PRNT90 test were followed from 2016 to 2018. The mean age of children at PRNT90 collection was 22 +/- 6 months Standard Deviation. The ZIKV PRNT90 was positive (titer >= 10) in 40 (42.5%) children. ZIKV PRNT90 positivity was associated with severe microcephaly in newborns (p = 0.016), lower head circumference z-score at birth (p = 0.043) and 24 months of age (p = 0.031), and severe reduction of the cerebral parenchyma volume (p = 0.021), expressing greater disease severity. Negative PRNT90 in children with characteristic signs of CZS may be due to false-negative results, indicating that the diagnosis of CZS should be primarily syndromic.