GUILHERME NADER MARTA
Projetos de Pesquisa
Unidades Organizacionais
LIM/55 - Laboratório de Urologia, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject Expression of peroxisome proliferator activated receptor gamma coactivator 1 (PGC-1) on the immune response to bacteria(2012) NERO, Luis Guilherme Del; MARTA, Guilherme; LIMA-SALGADO, Thais; KUBO, Sueli; LLIMONA, Flavia; VELASCO, Irineu Tadeu; SOUZA, HeraldoIntroduction/Objective: Inflammatory response during sepsis requires energy expenditure from immune cells. Since the transcription coactivator PGC-1 controls cell energy homeostasis, we aimed to determine whether PGC-1 may modulate the immune response in an experimental model of sepsis. Methods: Live bacteria were injected intraperitoneally in Balb/C mice and PGC-1β expression was evaluated by quantitative PCR in peritoneal macrophages. Further, mice (20 animals in each group) were submitted to cecal ligation and puncture (CLP). Group 1 received intraperitoneal injections of antisense oligonucleotide (ASO) against PGC-1β (3,0 nmol) three days before and three days after CLP. Group 2 was given no specific treatment. The animals were followed for 72 hours and mortality was evaluated every 12 hours. Results: Increased PGC-1β expression was observed in macrophages from animals exposed to bacteria compared to controls (2.7±0.5 vs. 1.0±0.2, respectively, p<0.05). We further evaluated whether PGC-1β absence would interfere with the response to acute bacterial aggression in vivo. Mice from Group 1, where PGC-1β expression was blocked, showed an 80% mortality after 72 hours, while animals from Group 2 had a mortality rate of 63% (p=0,7714). Conclusions: PGC-1β is up-regulated during bacterial infection, however, abolishing PGC-1β expression did not affect mortality of septic mice.