MARIA CLAUDIA COSTA IRIGOYEN

(Fonte: Lattes)
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Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina

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Colaboração internacional: Estados Unidos ×

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  • conferenceObject
    Improvement of the Adipokines Profile and Insulin Resistance in Metabolic Syndrome Patients Induced by Galantamine Activation of Cholinergic Pathway
    (2016) SANGALETI, Carine Teles; COSTA, Fernando Oliveira; MORAES, Tercio Lemos; IRIGOYEN, Maria Claudia; BORTOLOTTO, Luiz Aparecido Teles; LOPES, Heno Ferreira; PAVLOV, Valentin; TRACEY, Kevin; CONSOLIM-COLOMBO, Fernanda Marciano
  • conferenceObject
    The role of microRNA-208a in cardiac hypertrophy induced by aerobic physical training
    (2013) SOCI, Ursula Paula Reno; FERNANDES, Tiago; ROSA, Kaleizu T.; IRIGOYEN, Maria Claudia; PHILLIPS, M. Ian; OLIVEIRA, Edilamar Menezes de
    The miRNA-208 regulates gene expression of slow myosin heavy chain(MHC)in heart. We investigated the role of miRNA-208 and its target Thyroid Hormone Receptor Associated Protein 1 (THRAP1) in aerobic training (AT) induced cardiac hypertrophy (CH). Female Wistar Rats(n=21)into 3 groups: sedentary (S), Trained 1(T1): swimming sessions of 60', 5 days/week (10 weeks). Trained 2(T2): similar to T1 until 8th week, on the 9th week the rats swan twice/day and on the 10th week 3 times/day. Were assessed: heart rate (HR), blood pressure (BP) by direct measurement, VO2max by ergoespirometry, soleus citrate synthase (CS) activity by spectrophotometer, ventricular function and CH by echocardiography (EGC), CH by LV/BW ratio (mg/g) and cardiomyocyte diameter (CD), gene expression of atrial natriuretic factor (ANF), skeletal α-actin, α-MHC and β-MHC and miR-208 by Real-time PCR and protein content of α and β-MHC and THRAP1 by Western Blott. AT decreased HR and increased VO2max (11%/T1 and 15%/T2) and CS (47%/T1 and 105%/T2). LV/BW ratio increased 13%/T1 and 28%/T2 and CD increased 20%/T1 and 30%/T2. Gene expression of skeletal α-actin decreased 53%/T2. The E/A ratio increased and isovolumetric relaxation time and myocardial performance index decreased. MiR-208 expression decreased 62%/T2 with a decrease of β-MHC and an increase of α-MHC gene expression(β/-71%,α/+37%) and protein content(β/–45 % α/+72%) in T2. THRAP1 protein increased 40%/T2 compared with S. APT induced physiological CH and improved diastolic function. MiR-208a and β-MHC expression decreased and THRAP-1 increased. The decrease of miR-208 was opposite to disease and can be a therapeutic strategy in pathological CH.
  • conferenceObject
    Aerobic exercise training prevents autonomic impairment, oxidative stress and systemic inflammation in a model of aging, menopause e metabolic syndrome
    (2016) MACHI, J. F. M.; ALBUQUERQUE, O. A.; FREITAS, S. C. F.; CRUZ, P. L. C.; BARBOZA, C. A. B.; MORRIS, M. M.; ANGELIS, K. A. De; IROGOYEN, M. C. I.
  • article 43 Citação(ões) na Scopus
    Epigenetic control of exercise training-induced cardiac hypertrophy by miR-208
    (2016) SOCI, Ursula Paula Reno; FERNANDES, Tiago; BARAUNA, Valerio Garrone; HASHIMOTO, Nara Yumi; MOTA, Gloria de Fatima Alves; ROSA, Kaleizu Teodoro; IRIGOYEN, Maria Claudia; PHILIPS, Michael Ian; OLIVEIRA, Edilamar Menezes de
    Aerobic exercise-induced cardiac hypertrophy (CH) is a physiological response involving accurate orchestration of gene and protein expression of contractile and metabolic components. The microRNAs: miR-208a, miR-208b and miR-499 are each encoded by a myosin gene and thus are also known as 'MyomiRs', regulating several mRNA targets that in turn regulate CH and metabolic pathways. To understand the role of myomiRs in the fine-tuning of cardiac myosin heavy chain (MHC) isoform expression by exercise training-induced physiological hypertrophy, Wistar rats were subjected to two different swim training protocols. We observed that high-volume swim training (T2), improved cardiac diastolic function, induced CH and decreased the expression of miR-208a and miR-208b. Consequently, the increased expression of their targets, sex determining region y-related transcription factor 6 (Sox6), Med13, Pur beta, specificity proteins (Sp)/Kruppel-like transcription factor 3 (SP3) and HP1 beta (heterochromatin protein 1 beta) was more prominent in T2, thus converging to modulate cardiac metabolic and contractile adaptation by exercise training, with an improvement in the alpha-MHC/beta-MHC ratio, bypassing the increase in PPAR beta and histone deacetylase (HDAC) class I and II regulation. Altogether, we conclude that high-volume swim training finely assures physiological cardiac remodelling by epigenetic regulation of myomiRs, because inhibition of miR-208a and miR-208b increases the expression of their target proteins and stimulates the interaction among metabolic, contractile and epigenetic genes.
  • article 13 Citação(ões) na Scopus
    Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
    (2019) BARBOZA, C. A.; FUKUSHIMA, A. R.; CARROZZI, N.; MACHI, J. F.; DOURADO, P. M. M.; MOSTARDA, C. T.; IRIGOYEN, M. C.; NATHANSON, L.; MORRIS, M.; CAPERUTO, E. C.; RODRIGUES, B.
    Inflammatory processes and cardiovascular autonomic imbalance are very relevant characteristic of the enormous dynamic process that is a myocardial infarction (MI). In this sense, some studies are investigating pharmacological therapies using acetylcholinesterase inhibitors, such as pyridostigmine bromide (PYR), aiming to increase parasympathetic tone after MI. Here we hypothesized that the use of PYR before the MI might bring an additional positive effect to the autonomic function, and consequently, in the inflammatory response and cardiac function. The present study aimed to evaluate left ventricular function, baroreflex sensitivity, autonomic modulation, and inflammatory profile in PYR- treated rats previously to MI. Methods: Male Wistar rats (250-300 g) were treated for 60 days with PYR. After treatment, they were submitted to the MI. After the MI, the autonomic and ventricular function were evaluated, as well as the systemic, left ventricle, and adipose tissue inflammatory profile. Results: PYR, performed before MI, prevented HR increase, systolic function impairment, baroreflex sensitivity drop, as well as pulse interval variance, RMSSD, blood pressure and parasympathetic modulation reduction in treated rats compared to untreated rats. Also, this positive functional changes may have been a result of the reduced inflammatory parameters in the left ventricle (IFN-alpha, IL-6, and IL-1 beta), as well as increased IL-10 expression and IL-10/TNF-alpha ratio in treated animals before MI. Conclusion: Prior treatment with PYR prevents impairment of the autonomic nervous system after MI, which may be associated with the attenuated expression of inflammatory factors and heart dysfunction.
  • article 53 Citação(ões) na Scopus
    Positive effect of combined exercise training in a model of metabolic syndrome and menopause: autonomic, inflammatory, and oxidative stress evaluations
    (2015) CONTI, Filipe Fernandes; BRITO, Janaina de Oliveira; BERNARDES, Nathalia; DIAS, Danielle da Silva; MALFITANO, Christiane; MORRIS, Mariana; LLESUY, Susana Francisca; IRIGOYEN, Maria-Claudia; ANGELIS, Katia De
    It is now well established that after menopause cardiometabolic disorders become more common. Recently, resistance exercise has been recommended as a complement to aerobic (combined training, CT) for the treatment of cardiometabolic diseases. The aim of this study was to evaluate the effects of CT in hypertensive ovariectomized rats undergoing fructose overload in blood pressure variability (BPV), inflammation, and oxidative stress parameters. Female rats were divided into the following groups (n = 8/group): sedentary normotensive Wistar rats (C), and sedentary (FHO) or trained (FHOT) ovariectomized spontaneously hypertensive rats undergoing and fructose overload. CT was performed on a treadmill and ladder adapted to rats in alternate days (8 wk; 40-60% maximal capacity). Arterial pressure (AP) was directly measured. Oxidative stress and inflammation were measured on cardiac and renal tissues. The association of risk factors (hypertension + ovariectomy + fructose) promoted increase in insulin resistance, mean AP (FHO: 174 +/- 4 vs. C: 108 +/- 1 mmHg), heart rate (FHO: 403 +/- 12 vs. C: 352 +/- 11 beats/min), BPV, cardiac inflammation (tumor necrosis factor-alpha-FHO: 65.8 +/- 9.9 vs. C: 23.3 +/- 4.3 pg/mg protein), and oxidative stress cardiac and renal tissues. However, CT was able to reduce mean AP (FHOT: 158 +/- 4 mmHg), heart rate (FHOT: 303 +/- 5 beats/min), insulin resistance, and sympathetic modulation. Moreover, the trained rats presented increased nitric oxide bioavailability, reduced tumor necrosis factor-alpha (FHOT: 33.1 +/- 4.9 pg/mg protein), increased IL-10 in cardiac tissue and reduced lipoperoxidation, and increased antioxidant defenses in cardiac and renal tissues. In conclusion, the association of risk factors promoted an additional impairment in metabolic, cardiovascular, autonomic, inflammatory, and oxidative stress parameters and combined exercise training was able to attenuate these dysfunctions.
  • article 10 Citação(ões) na Scopus
    Exercise training initiated at old stage of lifespan attenuates aging-and ovariectomy-induced cardiac and renal oxidative stress: Role of baroreflex
    (2019) DIAS, Danielle da Silva; MORAES-SILVA, Ivana C.; BERNARDES, Nathalia; BRITO-MONZANI, Janaina de Oliveira; STOYELL-CONTI, Filipe Fernandes; MACHI, Jacqueline Freire; LLESUY, Susana; IRIGOYEN, Maria-Claudia; ANGELIS, Katia De
    Background: The association of aging and menopause is a potent risk factor for cardiometabolic disease. We studied the impact of aerobic exercise training (ET) initiated in the old stage of lifespan in hemodynamics, metabolic, autonomic and oxidative stress. Methods: Aged (18 months old) female Wistar rats were divided into: ovariectomized and untrained (AG-OVX), and ovariectomized and trained (AG-OVXt, ET for 8 weeks). Intact aged (AG) and young female rats (3 months old; Y) were also studied. Blood pressure and metabolic parameters were measured. Baroreflex sensitivity (BRS) was studied by bradycardic (BR) and tachycardic (TR) responses to vasoactive drugs. Cardiac and renal lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and gluthatione peroxidase (GPx), and gluthatione redox balance (GSH/GSSG) were analyzed. Results: AG-OVXt group increased aerobic performance in 35%, decreased adipose tissue and triglycerides in 36% and 27%, respectively, and improved insulin tolerance in 50% in comparison to AG-OVX. AG-OVX presented hypertensive levels of blood pressure (systolic: 155 +/- 5, diastolic: 111 +/- 3 mmHg). In contrast, AG-OVXt presented blood pressure values similar to Y rats (systolic: 129 +/- 3, diastolic: 112 +/- 3 mmHg). TR and BR were reduced by 70% and 46%, respectively, in AG-OVX vs. Y. Once more, AG-OVXt presented similar results to Y. ET decreased LPO in the heart and kidney. In the latter, renal CAT and SOD were corrected by ET, while cardiac redox balance was partially recovered. Improved BRS was correlated with improved oxidative stress markers. Conclusions: Even when initiated after aging and ovariectomy deleterious effects, ET is able to normalize BRS and highly improve cardiac and renal oxidative stress.
  • article 16 Citação(ões) na Scopus
    Baroreflex Impairment Precedes Cardiometabolic Dysfunction in an Experimental Model of Metabolic Syndrome: Role of Inflammation and Oxidative Stress
    (2018) BERNARDES, Nathalia; DIAS, Danielle da Silva; STOYELL-CONTI, Filipe Fernandes; BRITO-MONZANI, Janaina de Oliveira; MALFITANO, Christiane; CALDINI, Elia Garcia; ULLOA, Luis; LLESUY, Susana Francisca; IRIGOYEN, Maria-Claudia; ANGELIS, Katia De
    This study analyzes whether autonomic dysfunction precedes cardiometabolic alterations in spontaneously hypertensive rats (SHR) with fructose overload. Animals were randomly distributed into three groups: control, hypertensive and hypertensive with fructose overload. Fructose overload (100 g/L) was initiated at 30 days old, and the animals (n = 6/group/time) were evaluated after 7,15, 30 and 60 days of fructose consumption. Fructose consumption reduced baroreflex sensitivity by day 7, and still induced a progressive reduction in baroreflex sensitivity over the time. Fructose consumption also increased TNF alpha and IL-6 levels in the adipose tissue and IL-1 beta(3 levels in the spleen at days 15 and 30. Fructose consumption also reduced plasmatic nitrites (day 15 and 30) and superoxide dismutase activity (day 15 and 60), but increased hydrogen peroxide (day 30 and 60), lipid peroxidation and protein oxidation (day 60). Fructose consumption increased arterial pressure at day 30 (8%) and 60 (11%). Fructose consumption also induced a late insulin resistance at day 60, but did not affect glucose levels. In conclusion, the results show that baroreflex sensitivity impairment precedes inflammatory and oxidative stress disorders, probably by inducing hemodynamic and metabolic dysfunctions observed in metabolic syndrome.
  • article 30 Citação(ões) na Scopus
    Combined Aerobic and Resistance Exercise Training Improve Hypertension Associated With Menopause
    (2018) SHIMOJO, Guilherme Lemos; DIAS, Danielle da Silva; MALFITANO, Christiane; SANCHES, Iris Callado; LLESUY, Susana; ULLOA, Luis; IRIGOYEN, Maria-Claudia; ANGELIS, Katia De
    The prevalence of hypertension sharply increases in menopausal women. Recent studies have demonstrated that aerobic or resistance training may help control hypertension. In this study, we report that combining aerobic and resistance training may provide an effective therapeutic approach for hypertension control, attenuating inflammation and oxidative stress in ovariectomized rats. Female Wistar and spontaneous hypertensive rats (SHR) were distributed into four groups: sedentary control (C), sedentary hypertensive (HR), sedentary hypertensive ovariectomized (HRO), and combined trained hypertensive ovariectomized (T-HR-O). Combined exercise training was performed on a motor treadmill (aerobic training) and on a ladder adapted to rats (resistance training), in alternate days for 8 weeks. Direct arterial pressure was recorded and oxidative stress and inflammation were evaluated in cardiac and renal tissue. Ovariectomy increases increased mean arterial blood pressure, sympathetic modulation, and oxidative stress in SHR. Combining aerobic and resistance training reduced mean arterial blood pressure (12% vs. HR-O), heart rate (8% vs. HR-O), vascular sympathetic modulation (40% vs. HR-O), and improved baroreflex sensitivity. Combined training reduced cardiac inflammation (TNF and IL-6) and cardiac and renal lipoperoxidation (59% and 57%, respectively vs. HR-O). It also enhanced cardiac (71%) and renal (76%) total antioxidant capacity when compared to HRO group. In conclusion, combining aerobic and resistance training improves mean arterial blood pressure, cardiovascular autonomic control, preventing cardiac and renal oxidative stress and inflammation in an experimental hypertension model with surgical menopause induced with ovariectomy.
  • article 5 Citação(ões) na Scopus
    Impact of combined exercise training on the development of cardiometabolic and neuroimmune complications induced by fructose consumption in hypertensive rats
    (2020) DIAS, Danielle da Silva; BERNARDES, Nathalia; STOYELL-CONTI, Filipe Fernandes; SANTOS, Camila Paixao dos; ARAUJO, Amanda Aparecida de; LLESUY, Susana; INGOYEN, Maria Claudia; ANGELIS, Katie De
    This study evaluated the impact of combined exercise training on the development of cardiovascular and neuroimmune complications induced by fructose consumption (10% in the drinking water) in hypertensive rats (SHR). After weaning, SHR were divided into 3 groups: SHR (H), SHR+fructose (HF) and SHR+fructose+combined exercise training (treadmill+ladder, 40-60% of maximum capacity) (HFTC). Metabolic, hemodynamic, autonomic, inflammatory and oxidative stress parameters were evaluated in the subgroups (n = 6 group/time) at 7, 15, 30 and 60 days of protocol. Fructose consumption (H vs. HF groups) decreased spontaneous baroreflex sensitivity and total variance of pulse interval at day 7 (7 to 60); increased IL-6 and TNF alpha in the heart (at day 15, 30 and 60) and NADPH oxidase activity and cardiac lipoperoxidation (LPO) (day 60); increased white adipose tissue weight, reduced insulin sensitivity and increased triglycerides (day 60); induced an additional increase in mean arterial pressure (MAP) (days 30 and 60). Combined exercise training prevented such dysfunctions and sustained increased cardiac IL-10 (day 7) and glutathione redox balance (GSH/GSSG) for the entire protocol. In conclusion, combined exercise training performed simultaneously with exacerbated fructose consumption prevented early cardiovascular autonomic dysfunction, probably trigging positive changes in inflammation and oxidative stress, resulting in a better cardiometabolic profile in rats genetically predisposed to hypertension.