JULIANA MIKA KATO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/33 - Laboratório de Oftalmologia, Hospital das Clínicas, Faculdade de Medicina
LIM/33 - Laboratório de Oftalmologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Bactec (TM) blood culture bottles allied to MALDI-TOF mass spectrometry: rapid etiologic diagnosis of bacterial endophthalmitis(2017) TANAKA, Tatiana; OLIVEIRA, Luiza Manhezi de Freitas; FERREIRA, Bruno Fortaleza de Aquino; KATO, Juliana Mika; ROSSI, Flavia; CORREA, Karoline de Lemes Giuntini; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce Hisae; ALMEIDA JUNIOR, Joao NobregaMatrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been used for direct identification of pathogens from blood-inoculated blood culture bottles (BCBs). We showed that MALDI-TOF MS is an useful technique for rapid identification of the causative agents of endophthalmitis from vitreous humor-inoculated BCBs with a simple protocol.
conferenceObject Explanted Scleral Buckle, a retrospective study(2015) OLIVEIRA, Luiza Manhezi de Freitas; GIOIA, Thais Sabota Romano Di; ROSA, Valeria Teixeira Alves; ALMEIDA, Joao Nobrega de Nobrega de; ROSSI, Flavia; KATO, Juliana Mika; FERREIRA, Bruno Fortaleza de Aquino; TANAKA, Tatiana; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti- Surveillance of post-cataract endophthalmitis at a tertiary referral center: a 10-year critical evaluation(2021) KATO, Juliana Mika; TANAKA, Tatiana; OLIVEIRA, Luiza Manhezi Shin de; OLIVEIRA, Maura Salaroli de; ROSSI, Flavia; GOLDBAUM, Mauro; PIMENTEL, Sergio Luis Gianotti; ALMEIDA JUNIOR, Joao Nobrega de; YAMAMOTO, Joyce HisaeBackgroundAcute post-cataract endophthalmitis (APE) is a rare complication potentially causing irreversible visual loss. A 10-year study of APE was conducted to determine its incidence, microbiological spectra and antibiotic resistance profile of APE-related pathogens at a major tertiary referral center in Brazil.MethodsAPE cases reported between January 2010 and December 2019 were included. Phacoemulsification and extracapsular cataract techniques were eligible; combined procedures, traumatic and congenital cataract were excluded. Vitreous samples were cultured and antimicrobial resistance was compared for the periods of 2010-2014 and 2015-2019. The results were analyzed with Fisher's exact test.ResultsOur sample consisted of 40,491 cataract surgeries and 51 (0.126%) APE cases. Culture was positive in 35 cases (71.4%), of which 31 (88.6%) Gram-positive, 3 (8.6%) Gram-negative, and 1 (2.9%) fungal. The most frequently isolated organism was Staphylococcus epidermidis (n=17/35, 48.6%), followed by Staphylococcus aureus (n=4/35, 11.4%). From 2010-2014 to 2015-2019, antimicrobial resistance increased against moxifloxacin (11.1-54.5%, p=0.07), ciprofloxacin (54.5-72.7%, p=0.659) and oxacillin (66.7-93.3%, p=0.13).ConclusionsThe observed incidence and microbial spectra were compatible with previous studies. A trend towards growing moxifloxacin and ciprofloxacin resistance was observed. Surveillance remains crucial to prevent treatment failure from antimicrobial resistance.
conferenceObject Etiology and susceptibility pattern of isolates in endophthalmitis over a 5-year period(2015) KATO, Juliana Mika; OLIVEIRA, Maura Salaroli de; LEVIN, Anna Sara; ALMEIDA, Joao Nobrega de Nobrega de; ROSSI, Flavia; OLIVEIRA, Luiza Manhezi de Freitas; RUPPERT, Aline Domingos Pinto; TANAKA, Tatiana; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis GianotticonferenceObject The use of pediatric blood culture bottles in the diagnosis of acute postoperative endophthalmitis(2015) TANAKA, Tatiana; ALMEIDA, Joao Nobrega de; GIOIA, Thais Sabota Romano Di; ROSSI, Flavia; KATO, Juliana Mika; FERREIRA, Bruno Fortaleza de Aquino; RUPPERT, Aline Domingos Pinto; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce H.conferenceObject Bactec (TM) blood culture bottles allied to MALDI-TOF mass spectrometry: Rapid etiologic diagnosis of bacterial endophthalmitis(2017) TANAKA, Tatiana; OLIVEIRA, Luiza Manhezi de Freitas; FERREIRA, Bruno Fortaleza de Aquino; KATO, Juliana Mika; ROSSI, Flavia; CORREA, Karoline de Lemes Giuntini; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce H.; ALMEIDA JUNIOR, Joao Nobrega de- Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis(2019) TANAKA, Tatiana; FERREIRA, Bruno Fortaleza de Aquino; OLIVEIRA, Luiza Manhezi Shin de; KATO, Juliana Mika; GIOIA, Thais Sabato Romano Di; ROSSI, Flavia; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce Hisae; ALMEIDA JUNIOR, Joao Nobrega deOBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.