MARCELO TATIT SAPIENZA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina - Docente
Instituto de Radiologia, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
Instituto de Radiologia, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
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article 3 Citação(ões) na Scopus Correlação entre volume tireoidiano determinado pelo método de ultrassonografia versus cintilografia e sua implicação em cálculos dosimétricos na terapia com radioiodo na doença de Graves(2011) VIEIRA, Lucas de Oliveira; KUBO, Rodrigo; SAPIENZA, Marcelo Tatit; WILLEGAIGNON, Jose; CHAMMAS, Maria Cristina; COURA-FILHO, George Barberio; ONO, Carla Rachel; WATANABE, Tomoco; SADO, Heitor Naoki; BUCHPIGUEL, Carlos AlbertoIntroduction: Graves disease (GD) is the most common cause of hiperthyroidism, and the most common treatment options are surgery, antithyroid drugs and radioiodine therapy. In radiodosimetric calculations to determine radioiodine dosage it is possible to use thyroid volume estimatives based on ultrasound or scintigraphy. Objective: The present study aimed to correlate these methodologies emphasizing volume estimatives and dosimetric implications. Subjects and methods: Were included 103 patients with GD diagnosis and indication of radioiodine treatment. They were submitted to thyroid ultrasound and thyroid scintigraphy. Results and conclusions: Good correlation between both methods was observed, although scintigraphy systematically obtained greater volumes than ultrasound implying in lower estimatives of absorbed dose when scintigraphy is used. Arq Bras Endocrinol Metab. 2011;55(9):696-700- Short Dietary Intervention with Olive Oil Increases Brown Adipose Tissue Activity in Lean but not Overweight Subjects(2021) MONFORT-PIRES, Milena; U-DIN, Mueez; NOGUEIRA, Guilherme A.; ALMEIDA-FARIA, Juliana de; SIDARTA-OLIVEIRA, Davi; SANT'ANA, Marcella Ramos; LIMA-JUNIOR, Jose C. de; CINTRA, Dennys E.; SOUZA, Heraldo Possolo de; FERREIRA, Sandra R. G.; SAPIENZA, Marcelo Tatit; VIRTANEN, Kirsi A.; VELLOSO, Licio A.Background: The brown adipose tissue (BAT) is a potential target for the treatment of obesity and metabolic disorders. Its activation by cold exposure or adrenergic drugs can increase systemic insulin sensitivity and improve lipid metabolism; however, little is known about the effects of specific dietary components on BAT activity. Objectives: We asked if a short-term (4 weeks) dietary intervention with olive oil could modify BAT activity in lean and overweight/obese volunteers. Design: This was a 4-week open clinical trial in which all participants underwent a dietary intervention with extra-virgin olive oil supplementation. As the initial intake of olive oil was controlled all the participants were controls of themselves. Results: The intervention resulted in significant increase in blood monounsaturated fatty acid levels, which was accompanied by increased BAT activity in lean but not in overweight/obese volunteers. In the lean group, an increase in leptin was detected after the intervention, and low leptin values at the beginning of the study were predictive of greater BAT activity after intervention. In addition, increase in leptin concentration was associated with increased BAT activity. Three known endogenous mediators of BAT activity, secretin, fibroblast growth factor 21 (FGF21), and 12,13-dihydroxy-9Z-octadecenoic acid (12,13-diHOME) were increased by intervention in lean, whereas only secretin and FGF21 were increased in subjects with excessive weight. Conclusion: This study provides clinical evidence for the impact of monounsaturated fatty acids on BAT activity and an advance in the understanding of the beneficial health effects of olive oil.
- Effects of Thyroid Hormone Withdrawal and Recombinant Human Thyrotropin on Glomerular Filtration Rate During Radioiodine Therapy for Well-Differentiated Thyroid Cancer(2015) COURA-FILHO, George Barberio; WILLEGAIGNON, Jose; BUCHPIGUEL, Carlos Alberto; SAPIENZA, Marcelo TatitBackground: Renal function is related to thyroid hormonal status, and glomerular filtration rate (GFR) seems to be impaired in patients with hypothyroidism. The aim of this work was to evaluate quantitatively the effect of hypothyroidism on GFR using a Cr-51-EDTA radioisotope assay. Methods: Twenty-eight patients without known renal disease or dysfunction who had been referred for radioiodine therapy (RIT) after total thyroidectomy were enrolled in this study and divided into two groups. Group A underwent thyroid hormone withdrawal (THW) resulting in hypothyroidism, while group B underwent recombinant human thyrotropin (rhTSH) stimulation and hence remained euthyroid. GFR was assessed by Cr-51-EDTA before and after THW or rhTSH. Results: No clinical differences were observed between the two groups. The meanSD GFRs were 94 +/- 19mL/min/1.73m(2) before THW and 76 +/- 16mL/min/1.73m(2) after THW for group A (p=0.009), and 91 +/- 18mL/min/1.73m(2) before rhTSH and 93 +/- 15mL/min/1.73m(2) after rhTSH for group B (p=0.613). The percent decrease in GFR during hypothyroidism is approximately 18-22%. Conclusion: GFR decreases in patients with normal kidney function during THW for RIT, and rhTSH preserves GFR in these patients. This GFR impairment following thyroidectomy is related to hypothyroidism due to a significant reduction in thyroid hormone levels and is not due to a rise in the TSH level.
- Brown fat triglyceride content is associated with cardiovascular risk markers in adults from a tropical region(2022) MONFORT-PIRES, Milena; REGENI-SILVA, Giulianna; DADSON, Prince; NOGUEIRA, Guilherme A.; U-DIN, Mueez; FERREIRA, Sandra R. G.; SAPIENZA, Marcelo Tatit; VIRTANEN, Kirsi A.; VELLOSO, Licio A.Brown adipose tissue (BAT) is regarded as an interesting potential target for the treatment of obesity, diabetes, and cardiovascular diseases, and the detailed characterization of its structural and functional phenotype could enable an advance in these fields. Most studies evaluating BAT structure and function were performed in temperate climate regions, and we are yet to know how these findings apply to the 40% of the world's population living in tropical areas. Here, we used F-18-fluorodeoxyglucose positron emission tomography - magnetic resonance imaging to evaluate BAT in 45 lean, overweight, and obese volunteers living in a tropical area in Southeast Brazil. We aimed at investigating the associations between BAT activity, volume, metabolic activity, and BAT content of triglycerides with adiposity and cardiovascular risk markers in a sample of adults living in a tropical area and we showed that BAT glucose uptake is not correlated with leanness; instead, BAT triglyceride content is correlated with visceral adiposity and markers of cardiovascular risk. This study expands knowledge regarding the structure and function of BAT in people living in tropical areas. In addition, we provide evidence that BAT triglyceride content could be an interesting marker of cardiovascular risk.
- Absence of mutations in PAX8, NKX2.5, and TSH receptor genes in patients with thyroid dysgenesis(2012) BRUST, Ester S.; BELTRAO, Cristine B.; CHAMMAS, Maria C.; WATANABE, Tomoco; SAPIENZA, Marcelo T.; MARUI, SuemiObjectives: To precisely classify the various forms of TD, and then to screen for mutations in transcription factor genes active in thyroid development. Subjects and methods: Patients underwent ultrasound, thyroid scan, and serum thyroglobulin measurement to accurately diagnose the form of TD. DNA was extracted from peripheral leukocytes. The PAX8, and NKX2.5 genes were evaluated in all patients, and TSH receptor ( TSHR) gene in those with hypoplasia. Results: In 27 nonconsanguineous patients with TD, 13 were diagnosed with ectopia, 11 with hypoplasia, and 3 with athyreosis. No mutations were detected in any of the genes studied. Conclusion: Sporadic cases of TD are likely to be caused by epigenetic factors, rather than mutations in thyroid transcription factors or genes involved in thyroid development. Arq Bras Endocrinol Metab. 2012;56(3):173-7
- Radioguided localization of recurrent lymph nodes in differentiated thyroid cancer - Where are we now?(2018) SAPIENZA, Marcelo Tatit
- Melatonin Increases Brown Adipose Tissue Volume and Activity in Patients With Melatonin Deficiency: A Proof-of-Concept Study(2019) HALPERN, Bruno; MANCINI, Marcio C.; BUENO, Clarissa; BARCELOS, Isabella P.; MELO, Maria Edna de; LIMA, Marcos S.; CARNEIRO, Camila G.; SAPIENZA, Marcelo T.; BUCHPIGUEL, Carlos Alberto; AMARAL, Fernanda Gaspar do; CIPOLLA-NETO, JoseMelatonin, a pineal hormone synthesized at night, is critical for the synchronization of circadian and seasonal rhythms, being a key regulator of energy metabolism in many animal species. Although studies in humans are lacking, several reports, mainly on hibernating animals, demonstrated that melatonin supplementation and a short photoperiod increase brown adipose tissue (BAT) mass. The present proof-of-concept study is the first, to our knowledge, to evaluate BAT in patients with melatonin deficiency (radiotherapy or surgical removal of pineal gland) before and after daily melatonin (3 mg) replacement for 3 months. All four studied patients presented increased BAT volume and activity measured by positron emission tomography-MRI. We also found an improvement in total cholesterol and triglyceride blood levels without significant effects on body weight, liver fat, and HDL and LDL levels. Albeit not statistically significant, fasting insulin levels and HOMA of insulin resistance decreased in all four patients. The present results show that oral melatonin replacement increases BAT volume and activity and improves blood lipid levels in patients with melatonin deficiency, suggesting that melatonin is a possible BAT activator. Future studies are warranted because hypomelatoninemia is usually present in aging and appears as a result of light-at-night exposure and/or the use of beta-blocker drugs.
- Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by(18)F-FDG PET(2020) HALPERN, Bruno; MANCINI, Marcio C.; MENDES, Caroline; MACHADO, Camila Maria Longo; PRANDO, Silvana; SAPIENZA, Marcelo Tatit; BUCHPIGUEL, Carlos Alberto; AMARAL, Fernanda Gaspar do; CIPOLLA-NETO, JoseObjective Melatonin has been shown to increase brown adipose tissue (BAT) mass, which can lead to important metabolic effects, such as bodyweight reduction and glycemic improvement. However, BAT mass can only be measured invasively and. The gold standard for non-invasive measurement of BAT activity is positron emission tomography with 2-deoxy-2-[fluorine-18] fluoro-d-glucose (F-18-FDG PET). There is no study, to our knowledge, that has evaluated if melatonin influences BAT activity, measured by this imaging technique in animals. Methods Three experimental groups of Wistar rats (control, pinealectomy, and pinealectomy replaced with melatonin) had an(18)F-FDG PET performed at room temperature and after acute cold exposure. The ratio of increased BAT activity after cold exposure/room temperature was called ""acute thermogenic capacity"" (ATC) We also measured UCP-1 mRNA expression to correlate with the(18)F-FDG PET results. Results Pinealectomy led to reduced acute thermogenic capacity, compared with the other groups, as well as reduced UCP1 mRNA expression. Conclusion Melatonin deficiency impairs BAT response when exposed to acute cold exposure. These results can lead to future studies of the influence of melatonin on BAT, in animals and humans, without needing an invasive evaluation of BAT.