JOSE ANGELO LAULETTA LINDOSO
Projetos de Pesquisa
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LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
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bookPart 0 Citação(ões) na Scopus Parasitological, serological diagnosis of caninevisceral leishmaniasis, association of neutrophilic inflammatory infiltrate and transmission potential: A review(2013) COSTA, F. A. L.; CARVALHO, A. A.; LINDOSO, J. A. L.; GOTO, H.Canine visceral leishmaniasis (CVL) is important in the transmission cycle of Leishmania (Leishmania) infantum as it constitutes the main reservoir of the parasite in the peridomestic cycle. We revise the laboratory diagnosis of CVL since control program in some parts of the world including Brazil has in its guideline the culling of infected dogs based on anti-Leishmania antibody detection. But the serological techniques have limitation regarding the specificity and sensitivity. The confirmation of infection is performed by parasitological analysis that is considered the gold standard since it presents 100% specificity. Whereas sensitivity, specificity and predictive values of the parasitological methods are critical aspects for the diagnosis of CVL per se and for the dogs as source of parasite for transmission, parameters that may give indication of this potential are important to unveil. Here we discuss the serological diagnosis and then the parasitological diagnosis with focus on neutrophilic infiltrate in the tissue related to transmission potential. © 2013 Nova Science Publishers, Inc. All rights reserved.- APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL(2013) SATOW, Marcela M.; YAMASHIRO-KANASHIRO, Edite H.; ROCHA, Mussya C.; OYAFUSO, Luiza K.; SOLER, Rita C.; COTRIM, Paulo C.; LINDOSO, Jose Angelo L.This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emolio Ribas (IIER), a reference center for infectious diseases in Sao Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER.
- 5-Nitro-2-furfuriliden derivatives as potential anti-Trypanosoma cruzi agents: Design, synthesis, bioactivity evaluation, cytotoxicity and exploratory data analysis(2013) PALACE-BERL, Fanny; JORGE, Salomao Doria; PASQUALOTO, Kerly Fernanda Mesquita; FERREIRA, Adilson Kleber; MARIA, Durvanei Augusto; ZORZI, Rodrigo Rocha; BORTOLOZZO, Leandro de Sa; LINDOSO, Jose Angelo Lauletta; TAVARES, Leoberto CostaThe anti-Trypanosoma cruzi activity of 5-nitro-2-furfuriliden derivatives as well as the cytotoxicity of these compounds on J774 macrophages cell line and FN1 human fibroblast cells were investigated in this study. The most active compounds of series I and II were 4-butyl-[N'-(5-nitrofuran-2-y1) methylene] benzidrazide (3g; IC50= 1.05 mu M +/- 0.07) and 3-acety1-5-(4-butylpheny1)-2-(5-nitrofuran-2-y1)-2,3-dihydro,1,3,4-oxadiazole (4g; IC50 = 8.27 mu M +/- 0.42), respectively. Also, compound 3g was more active than the standard drugs, benznidazole (IC50= 22.69 u mu M 1.96) and nifurtimox (IC50= 3.78 mu M +/- 0.10). Regarding the cytotoxicity assay, the 3g compound presented IC50 value of 28.05 mu M (SI = 26.71) against J774 cells. For the FN1 fibroblast assay, 3g showed IC50 value of 98 mu M (SI = 93.33). On the other hand, compound 4g presented a cytotoxicity value on J774 cells higher than 400 mu M (SI >48), and for the FN1 cells its IC50 value was 186 mu M (SI = 22.49). Moreover, an exploratory data analysis, which comprises hierarchical cluster (HCA) and principal component analysis (PCA), was carried out and the findings were complementary. The molecular properties that most influenced the compounds' grouping were ClogP and total dipole moment, pointing out the need of a lipophilic/hydrophilic balance in the designing of novel potential anti-T. cruzi molecules.
- Microbial Translocation Induces an Intense Proinflammatory Response in PatientsWith Visceral Leishmaniasis and HIV Type 1 Coinfection(2013) SANTOS-OLIVEIRA, Joanna R.; REGIS, Eduardo G.; GIACOIA-GRIPP, Carmem B. W.; VALVERDE, Joanna G.; ALEXANDRINO-DE-OLIVEIRA, Priscilla; LINDOSO, Jose Angelo L.; GOTO, Hiro; OLIVEIRA-NETO, Manoel P.; GUERRA, Jorge O.; GRINSZTEJN, Beatriz; JERONIMO, Selma B.; MORGADO, Mariza G.; DA-CRUZ, Alda M.Background. Leishmania infection is a cofactor in the heightened cellular activation observed in patients with American visceral leishmaniasis and human immunodeficiency virus type 1 (HIV) infection, with or without progression to AIDS (AVL/HIV). Thus, the persistence of a high parasite load despite antileishmanial therapy could be responsible for the continued immune stimulation. Methods. CD8(+) T cells expressing CD38, parasite load, lipopolysaccharide (LPS), soluble CD14, macrophage migration inhibitory factor (MIF), intestinal fatty acid-binding protein (IFABP), and proinflammatory cytokines (interleukin 1 beta, interleukin 6, interleukin 8, interleukin 17, interferon gamma, and tumor necrosis factor) were measured in 17 patients with AVL/HIV, 16 with HIV, and 14 healthy subjects (HS). Results. Lower Leishmania parasitemia was observed after antileishmanial and antiretroviral therapies. However, higher levels of CD38(+) on CD8(+) T cells were observed in both clinical phases of leishmaniasis, compared with HIV cases. AVL/HIV and HIV patients showed higher levels of LPS and IFABP than HS. Proinflammatory cytokine levels were significantly augmented in patients with active coinfection, as well as those with remission of Leishmania infection. LPS levels and Leishmania infection were positively correlated with CD38 expression on CD8(+) T cells and with IL-6 and IL-8 levels. Conclusions. LPS levels along with the immune consequences of Leishmania infection were associated with elevated cellular activation in coinfected patients. As a consequence, secondary chemoprophylaxis for leishmaniasis or even the use of antiinflammatory drugs or antibiotics may be considered for improving the prognosis of AVL/HIV.
bookPart Doenças regionais negligenciadas na coinfecção com HIV(2013) LINDOSO, José Angelo Lauletta; POSADA-VERGARA, Maria PaulinaconferenceObject UPTAKE OF METHYENE BLUE PHOTOSENSITIZER IN LEISHMANIA AMAZONENSIS(2013) AURELIANO, Debora; LINDOSO, Jose Angelo; NUNEZ, Silvia; RIBEIRO, Martha