KATIA RAMOS MOREIRA LEITE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cirurgia, Faculdade de Medicina - Docente
LIM/55 - Laboratório de Urologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/55 - Laboratório de Urologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
19 resultados
Resultados de Busca
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- Increased expression of MMP-9 and IL-8 are correlated with poor prognosis of Bladder Cancer(2012) REIS, Sabrina Thalita; LEITE, Katia Ramos M.; PIOVESAN, Luis Felipe; PONTES-JUNIOR, Jose; VIANA, Nayara Izabel; ABE, Daniel Kanda; CRIPPA, Alexandre; MOURA, Caio Martins; ADONIAS, Sanarelly Pires; SROUGI, Miguel; DALL'OGLIO, Marcos FranciscoBackground: Extracellular matrix homeostasis is strictly maintained by a coordinated balance between the expression of metalloproteinases (MMPs) and their inhibitors. The purpose of this study was to investigate whether the expression of MMP-9, MMP-2 and its specific inhibitors, are expressed in a reproducible, specific pattern and if the profiles are related to prognosis in Bladder Cancer (BC). Methods: MMP-9, MMP-2 and its specific inhibitors expression levels were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) in fresh-frozen malignant tissue collected from 40 patients with BC submitted to transurethral resection of bladder. The control group consisted of normal bladder tissue from five patients who had undergone retropubic prostatectomy to treat benign prostatic hyperplasia. Results: MMP-9 was overexpressed in 59.0 % of patients, and MMP-2, TIMP-1, TIMP-2, MMP-14, RECK and IL-8 was underexpressed in most of the patients. Regarding prognostic parameters we observed that high-grade tumors exhibited significantly higher levels of MMP-9 and IL-8 (p = 0.012, p = 0.003). Invasive tumors (pT1-pT2) had higher expression levels of MMP-9 than superficial tumors (pTa) (p = 0.026). The same was noted for IL-8 that was more expressed by invasive tumors (p = 0.015, p = 0.048). Most importantly tumor recurrence was related with higher levels of both MMP-9 (p = 0.003) and IL-8 (p = 0.005). Conclusion: We have demonstrated that the overexpression of MMP-9 and higher expression of IL-8 are related to unfavorable prognostic factors of urothelial bladder cancer and tumor recurrence and may be useful in the follow up of the patients.
- Prostate biopsy in patients with long-term use of indwelling bladder catheter: What is the rationale?(2012) ANTUNES, Alberto A.; BARBOSA, Joao Arthur B. A.; REIS, Sabrina T.; GUARIERO, Mary S.; FUKUSHIMA, Julia T.; DALL'OGLIO, Marcos F.; FREIRE, Geraldo de C.; LUCON, Antonio M.; LEITE, Katia R.; SROUGI, MiguelObjective: Acute urinary retention (AUR) is expected to occur in 2% to 39% men with benign prostatic hyperplasia. To date, no study has elucidated the effect of long-term use of indwelling bladder catheter on serum prostate specific antigen (PSA) levels and on the incidence of prostate cancer (CaP). The aim of the present study is to analyze the incidence of CaP in patients with long-term use of indwelling bladder catheter and determine some practice patterns on this issue. Materials and methods: The study comprised a retrospective analysis of data from 1,651 patients who had undergone transrectal ultrasound (TRUS)-guided prostate biopsy from July 2004 to June 2009. Among these patients, 198 (12%) were using an indwelling bladder catheter during the biopsy for at least 1 month. The incidence of CaP was recorded according to total PSA levels. Other variables such patient age, free/total PSA rate, PSA density, prostate volume, and duration of catheter use was also analyzed. Men with a digital rectal examination suspicious for cancer were not considered for analysis. Results: Median patient age was 71 years (37 to 89 years). Overall, 25% of patients presented a CaP diagnosis. CaP incidence according to the PSA levels was 0%, 18.9%, 24.5%, and 40.6% for patients with PSA <= 4.0, 4.1-10.0, 10.1-20.0, and >20.0 ng/ml, respectively. When prostate volume was analyzed together, we demonstrated that only 1 (2.4%) patient with PSA below 10.0 ng/ml and prostate volume >60 g had CaP. Median total PSA, PSA density, and prostate volume were statistically different between patients with and without CaP. Conclusions: Prostate biopsy should not be indicated for all patients with diagnosis of BPH and AUR who present an elevated PSA level. Patients with PSA below 10.0 ng/ml, and prostate volume >60 g should only undergo biopsy in selected cases. Patients with PSA >20.0 ng/ml and a prostate volume <= 60 g are at higher risk of CaP diagnosis.
- GREB1 tissue expression is associated with organ-confined prostate cancer(2012) ANTUNES, Alberto A.; LEITE, Katia R.; REIS, Sabrina T.; SOUSA-CANAVEZ, Juliana M.; CAMARA-LOPES, Luiz H.; DALL'OGLIO, Marcos F.; SROUGI, MiguelObjective: By reason of its heterogeneous behavior, it is difficult to determine the prognosis of many prostate cancer cases. Patients with the same clinicopathologic conditions may present varying clinical findings and rates of progression. We determined the role of new genes as potential molecular markers for prostate cancer prognosis. Materials and methods: We performed a microarray analysis of two pools of patients with prostate cancer divided according to their clinicopathologic characteristics. After that, we validated these results by testing the genes with most different expressions between the two pools using the quantitative real time polymerase chain reaction method. We analyzed gene expression in 33 patients with localized prostate cancer according to prostate specific antigen (PSA), pathologic stage, Gleason score, and biochemical recurrence. For statistical analysis we used the Mann-Whitney Test. Results: The microarray analysis revealed that 4,147 genes presented a different expression between the two pools. Among them, 3 genes, TMEFF2, GREB1, and THIL,, were at least 13-times overexpressed, and 1 gene, IGH3, which was at least 5times under-expressed in pool 1 (good prognosis) compared with pool 2 (bad prognosis), were selected for analysis. After the validation tests, GREB1 was significantly more overexpressed among patients with stage T2 compared with T3 (P = 0.020). The expressions of other 3 genes did not present significant differences according to the clinicopatholoOcal variables. Conclusions: Tissue expression of GREB1 is associated with organ-confined prostate cancer and may constitute a gene associated with a favorable prognosis.
article 3 Citação(ões) na Scopus Influence of a hypercholesterolemic diet on the collagen composition of the bladder wall extracellular matrix in rats(2012) NUNES, R. L. V.; BRUSCHINI, H.; UTSUNOMIA, K.; SILVEIRA, M. A.; TEODORO, W. R.; LEITE, K. R. M.; SROUGI, M.Purpose: To investigate the effects of hypercholesterolemic diet on the collagen composition of urinary bladder wall. Materials and methods: Forty-five female 4-week-old Wistar rats were divided into three groups: 1) control group fed a normal diet (ND); 2) model of bladder outlet obstruction (BOO) group fed a ND; and 3) group fed a HCD (1.25% cholesterol). Total serum cholesterol, LDL cholesterol and body weight were assessed at baseline. Four weeks later, group 2 underwent a surgical procedure resulting in a partial BOO, while groups 1 and 3 underwent a sham similar surgical procedure. Six weeks later, all animals had their bladders removed; serum cholesterol and LDL cholesterol levels and body weights were measured. Morphological and morphometric analysis was performed by Picrosirius staining and collagen types I and III were identified by immunofluorescence. Statistical analysis was completed and significance was considered when p<0.05. Results: Rats fed an HCD exhibited a significant increase in LDL cholesterol levels (p<0.001) and body weight (p=0.017), when compared to the groups fed a ND during the ten-week study period. Moreover, the HCD induced morphological alterations of the bladder wall collagen, regarding thin collagen fibers and the amounts of type III collagen when compared to the control group (p=0.002 and p=0.016, respectively), resembling the process promoted in the BOO model. Conclusions: A hyper-cholesterolemic diet in Wistar rats promoted morphological changes of the bladder types of collagen, as well as increases in body weight and LDL cholesterol.conferenceObject MICRO RNA PROFILE OF SUPERFICIAL AND INVASIVE BLADDER UROTHELIAL CARCINOMAS RELATED TO DISEASE-FREE AND CANCER-SPECIFIC SURVIVAL(2012) DIP, Nelson; REIS, Sabrina; ABE, Daniel; DALLOGLIO, Marcos; SROUGI, Miguel; LEITE, Katia- Phoneutria nigriventer spider toxin Tx2-6 causes priapism and death: A histopathological investigation in mice(2012) LEITE, Katia R. M.; ANDRADE, Enrico; RAMOS, Adriana T.; MAGNOLI, Fabio C.; SROUGI, Miguel; TRONCONE, Lanfranco R. P.Phoneutria nigriventer spider bite causes priapism, an effect attributed to the peptide toxins Tx2-5 and Tx2-6 and involving nitric oxide. Tx2-6 (MW = 5287) is known to delay the inactivation of Sodium channels in the same fashion as many other venom toxins. In the present study we evaluated the i.p. dose that induces priapism and the other symptoms in mice. Animals killed by the toxin or crude venom (0.85 mg/kg) were autopsied and a pathological study of brain, lung, kidney, liver and heart was undertaken using standard techniques. The same protocol was employed with animals injected with crude venom. Results showed that priapism is the first sign of intoxication, followed by piloerection, abundant salivation and tremors. An i.p. injection of about 0.3 mu g/kg induced only priapism with minimal side-effects. The most remarkable histological finding was a general vascular congestion in all organs studied. Penis showed no necrosis or damage. Lungs showed vascular congestion and alveolar hemorrhage. Heart showed also sub-endothelial hemorrhage. Brain showed only a mild edema and vascular congestion. Results obtained with crude venom closely resemble those of purified toxin. We conclude that Tx2-6 have profound effects on the vascular bed especially in lungs and heart, which may be the cause of death. Interestingly brain tissue was less affected and the observed edema may be attributed to respiratory impairment. To the best of our knowledge this is the first histopathological investigation on this toxin and venom suggesting a possible cause of death.
conferenceObject OCCUPATIONAL RISK FOR INITIAL BLADDER CANCER DIAGNOSIS IN BRAZIL(2012) ADONIAS, Sanarelly; CHADE, Daher C.; SANT'ANNA, Alexandre C.; REIS, Sabrina T.; ABE, Daniel K.; AREAS, Flavio G. M.; SUZAKI, T. T.; LEITE, Katia R.; SROUGI, Miguel; DALL'OGLIO, Marcos F.conferenceObject UNDER-EXPRESSION OF MICRORNA-155 MAY INHIBIT PROSTATE GROWTH THROUGH SMAD-2 PATHWAY IN PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA(2012) ANTUNES, Alberto A.; REIS, Sabrina; NAKANO, Elcio; BIOLO, Karlo; PARANHOS, Mario; CORDEIRO, Paulo; FREIRE, Geraldo Campos; LEITE, Katia; SROUGI, MiguelconferenceObject THE ROLE OF MICRORNA-126 AND SPRED1 IN BENIGN PROSTATIC HYPERPLASIA(2012) ANTUNES, Alberto A.; REIS, Sabrina; NAKANO, Elcio; BIOLO, Karlo; PARANHOS, Mario; CORDEIRO, Paulo; FREIRE, Geraldo Campos; LEITE, Katia; SROUGI, Miguel- Could Raman spectroscopy discriminate the biochemical alterations among prostate carcinoma and benign prostate tissues? An in vitro study(2012) SILVEIRA JR., Landulfo; LEITE, Katia Ramos M.; SROUGI, Miguel; SILVEIRA, Fabricio L.; PACHECO, Marcos Tadeu T.; PASQUALUCCI, Carlos A.This work evaluated possible alterations in the Raman spectra of human prostate tissues in vitro, by developing a Principal Components Analysis algorithm for discriminating prostate carcinoma (PrCa) and benign prostate tissue. Raman spectrum was measured using a Raman spectrometer (830 nm) with a fiber Raman probe. Most of the samples exhibited a very strong background fluorescence, which was decreased by photobleaching the fragment during 5 min. and the remaining fluorescence was removed by polynomial filtering. The spectra of prostate in the fingerprint region are dominated by bands of proteins (mainly collagen, elastin, actin). By comparing the spectra of PrCa with the benign prostate tissue, we found a very small difference, indicating similar biochemical constitution of benign and malignant prostate tissue. Principal Components Analysis and Mahalanobis distance could discriminate the spectra of both benign and PrCa tissues with reasonable sensitivity and specificity.