LAURA CRISTINA SICHERO VETTORAZZO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Betapapillomavirus natural history and co-detection with alphapapillomavirus in cervical samples of adult women(2023) MALAGON, Talia; RIBEIRO, Aline Lopes; NUNES, Emily Montosa; GHEIT, Tarik; EL-ZEIN, Mariam; VILLA, Luisa L.; FRANCO, Eduardo L.; SICHERO, LauraHuman papillomaviruses (HPV) of the genus Betapapillomavirus can infect both cutaneous and mucosal sites, but research on their natural history at mucosal sites remains scarce. We examined the risk factors and co-detection patterns of HPVs of the Betapapillomavirus and Alphapapillomavirus genera in cervical samples of the Ludwig-McGill cohort study. We assessed a subset of 505 women from the Ludwig-McGill cohort study from Sao Paulo, Brazil. Cervical samples over the first year of follow-up were tested for DNA of over 40 alphapapillomavirus types and 43 betapapillomavirus types using a type-specific multiplex genotyping polymerase chain reaction assay. We assessed the risk factors for prevalent and incident betapapillomavirus type detection, and whether types were detected more frequently together than expected assuming independence using permutation tests, logistic regression, and Cox regression. We observed significant within-genus clustering but not cross-genus clustering. Multiple betapapillomavirus types were co-detected in the same sample 2.24 (95% confidence interval [CI]: 1.65-3.29) times more frequently than expected. Conversely, co-detections of alphapapillomavirus and betapapillomavirus types in the same sample occurred only 0.64 (95% CI: 0.51-0.83) times as often as expected under independence. In prospective analyses, positivity to one HPV genus was associated with a nonsignificant lower incidence of detection of types in the other genus. Lifetime number of sex partners and new sex partner acquisition were associated with lower risks of prevalent and incident betapapillomavirus detection. Betapapillomaviruses are commonly found in the cervicovaginal tract. Results suggest potentially different mechanisms of transmission for betapapillomavirus genital infections other than vaginal sex.
- Human Papillomavirus 16 Lineage A Variants Associated With Persistent Genital Infections in Men: The HPV Infection in Men (HIM) Study(2023) FERREIRA, Matthew Thomas; LOPEZ, Rossana Veronica Mendoza; GONCALVES, Milena Giulia; FERREIRA, Silvaneide; SIRAK, Bradley; BAGGIO, Maria Luizai; LAZCANO-PONCE, Eduardo; NYITRAY, Alan G.; GIULIANO, Anna R.; VILLA, Luisa L.; SICHERO, Laura; LIN, Huiyi; MESSINA, Jane; CAMPBELL, Christine Pierce; GAGE, Christine; INGLES, Donna J.; ISAACS, Kim; KENNEDY, Kayoko; BOBANIC, Andrea; RAHMAN, Shams; SCHABATH, Matthew; NYITRAY, Alan; RATHWELL, Julie; PAULA, Lenice Galan de; CINTRA, Ricardo; CERNICCHIARO, Filomena; RIBEIRO, Graca; OTERO, Rosaria; BOCALON, Roberta; ANTUNES, Juliana; SILVA, Fernanda; TERRERI, Rossana; VALDEZ, Aurelio Cruz; VASQUEZ, Rene de Jesus Alvear; JUAREZ, Oscar Rojas; SOSA, Rossana del Carmen Gonzalez; VENCES, Rosangel Rios; SEGURA, Martha Huerta; GALVAN, Alicia Rodriguez; RODRIGUEZ, Paula Roman; VELEZ, Ana Laura Landa; GARCIA, Griselda Diaz; ABARCA, Veronica Chavez; QUEVEDO, Gisela Flores; NEVAREZ, Maria del Pilar Hernandez; MARTINEZ, Guillermina Sanchez; ROJAS, Adriana Ortiz; FLORES, Carlos Omar Barrera; MANGONE, Flavia Rotea; PAVANELLI, Ana CarolinaWe show for the first time the influence of human papillomavirus (HPV) 16 nucleotide variability on the risk of persistent infection in the male genitalia. Our data suggest differences in the natural history of HPV-16 variants between men and women. Background Human papillomavirus (HPV) 16 non-A lineage variants have higher carcinogenic potential for cervical cancer. HPV-16 variants natural history among males is not established. We evaluated HPV-16 variants prevalence and persistence in the external genitalia of men enrolled in the prospective HPV Infection in Men (HIM) Study. Methods The HIM Study included men from the United States, Brazil, and Mexico. HPV-16 variants were distinguished using polymerase chain reaction sequencing. The prevalence of HPV-16 variants was assessed, and associations with infection persistence were estimated. Results We characterized the HPV-16 variants for 1700 genital swab samples from 753 men and 22 external genital lesions in 17 men. The prevalence of HPV-16 lineages differed by country and marital status (P < .001). Overall, 90.9% of participants harbored lineage A variants. The prevalence of non-A lineages was heterogenous among countries. HPV-16 lineage A variants were associated with a 2.69-fold increased risk of long-term persistent infections compared with non-A lineages. All high-grade penile intraepithelial neoplasia harbored lineage A variants and occurred in the context of long-term persistent infections with the same variants. Conclusions The prevalence and persistence of HPV-16 variants observed at the male external genitalia suggest differences in the natural history of these variants between men and women, which may be associated with intrinsic differences in the infected genital epithelia.