MILTON DE ARRUDA MARTINS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
41 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 41
conferenceObject Central Insulin Regulates Airways Reactivity by ERK1/2 Pathway in Cholinergic Neurons(2014) LEIRIA, Luiz; ARANTES-COSTA, Fernanda; MOURA, Rodrigo; DONATO JR., Jose; ANTUNES, Edson; VELLOSO, Licio A.; MARTINS, Milton Arruda; SAAD, Mario J. A.conferenceObject Sometimes Less Is More: A Compare Between Two Models Of Cigarette Smoke Co-Exposition In Ova-Sensitized Mice(2014) ARANTES-COSTA, F. M.; BRUGGEMANN, T. R.; FERNANDES, P.; OLIVEIRA, J. M. S.; SARAIVA-ROMANHOLO, B. M.; MARTINS, M. A.- Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice (vol 8, 12636, 2018)(2018) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; HIZUME-KUNZLER, Deborah Camargo; MORIYA, Henrique Takachi; SILVA, Ronaldo Aparecido; CRUZ, Joao Carlos Goncalves; LOPES, Renan Boeira; RIGHETTI, Renato Fraga; VIEIRA, Rodolfo de Paula; SAIKI, Mitiko; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; ARANTES-COSTA, Fernanda Magalhaes; SARAIVA-ROMANHOLO, Beatriz Mangueira
- Inflammation and Remodeling in Infantile, Juvenile, and Adult Allergic Sensitized Mice(2011) CARNIELI, Denise S.; YOSHIOKA, Eliane; SILVA, Luiz Fernando F.; LANCAS, Tatiana; ARANTES, Fernanda M.; PERINI, Adenir; MARTINS, Milton A.; SALDIVA, Paulo Hilario N.; DOLHNIKOFF, Marisa; MAUAD, ThaisBackground: Airway structural changes occur early in childhood asthma, but it is unknown whether the development of airway alterations in children is similar to that of adults. We compared inflammation and remodeling parameters in allergic sensitized infantile, juvenile, and adult mice. Methods: Infantile mice (18D) were sensitized with three intraperitoneal injections (i.p.) of ovalbumin (OVA) at days 5 and 7 and challenged with OVA at days 14-16. The 18D1 group received an additional challenge at days 9-11. The juvenile mice (40D) received challenges at days 22-24 and 36-38. Adult mice (100D) were sensitized at days 60-62 and received three inhalations at days 77-79 and 96-98. Animals were submitted to whole body plethysmography. Airway eosinophils, CD3+ T-lymphocytes, IL-5+ cells, mucus content, collagen and reticular fibers density, and smooth muscle thickness were quantified. Results: All sensitized animals presented with airway hyperresponsiveness, without differences in eosinophil cell density The density of CD3+ T-cells was higher in the 100D and 1801 groups than in the 18D and 40D groups. Infantile sensitized groups demonstrated increased interleukin-5 expression in the airways. Infantile mice demonstrated more mucus in the bronchiolar epithelium than the 40D and 100D mice. The 18D animals demonstrated less collagen than the 18D1 group. Juvenile and adult mice had increased airway smooth muscle thickness when compared to age-matched controls, but no differences were observed in the infantile groups. Conclusion: We have shown that infantile mice develop inflammatory and structural alterations in the airways that are partially different from those developed in older animals. Pediatr Pulmonol. 2011;46:650-665. (C) 2011 Wiley-Liss, Inc.
conferenceObject Effect Of Proteinase Inhibitor Of Plant Origin Cratabl In An Experimental Model Of Chronic Allergic Pulmonary Inflammation(2014) SANTOS, A. S. A. Dos; RODRIGUES, A. P. D.; ARANTES-COSTA, F. M.; SARAIVA-ROMANHOLO, B. M.; FILHO, G. G. N.; LEICK, E. A.; MARTINS, M. A.; TIBERIO, I. D. F. L. C.- Gender differences in the perception of quality of life during internal medicine training: a qualitative and quantitative analysis(2018) KOBAYASI, Renata; TEMPSKI, Patricia Zen; ARANTES-COSTA, Fernanda Magalhaes; MARTINS, Milton ArrudaBackgroundThe higher level of participation by women in medicine may impact this profession's evolution due to gender differences perceived during medical school, after graduation and during residency. Gender differences regarding quality of life are associated with higher states of anxiety and depression among female physicians.We aimed to assess gender differences in the perception of quality of life with quantitative methods and to understand further, from the female residents point of view, the reasons that may influence the perception of quality of life using qualitative method. Resilience, empathy and daytime sleepiness were also scored.MethodsWe performed a cross-sectional study with first-year internal medicine residents to evaluate self-reported quality of life factors specific to medical residents (VERAS-Q), including empathy (Jefferson Scale of Empathy), resilience (Wagnild and Young Brief Resilience Scale) and daytime sleepiness (Epworth Scale). We explored, from the female residents view which factors may influence the perception of quality of life using a focus group method.ResultsIn our study, one hundred and nine residents completed the survey: 31 (28.4%) were female and 78 (71.6%) were male. Female residents exhibited significantly lower scores than those of male residents for quality of life in the domains of time management (30.3, females vs 41.1, males; p<0.001), psychology (48.1, females vs 56.7, males; p<0.01) and physical health (42.8, females vs 53.6, males; p<0.05). Female residents also scored higher for daytime sleepiness (13.0, females vs 9.0, males; p<0.001), with pathological scores for daytime sleepiness. No significant gender differences were found in the resilience or empathy scores. The focus group assessment revealed difficulty in concentration and knowledge acquisition, insecurity, feelings of loss, greater critical perception, self-doubt and difficulty in creating effective bonds to support the training period as the main factors involved in the lower perception of quality of life among the women.ConclusionsIn conclusion, female residents had lower scores for quality of life and higher scores for daytime sleepiness. Measures to improve quality of life among female residents during this critical period of medical training might include investing in mentoring to help them better manage their time and encouraging activities that facilitate relationship development.
- Tolerogenic microenvironment in neonatal period induced by maternal immunization with ovalbumin(2014) MUNIZ, Bruno Pacola; VICTOR, Jefferson Russo; OLIVEIRA, Luana de Mendonca; LIRA, Aline Aparecida de Lima; PERINI, Adenir; OLIVO, Clarice Rosa; ARANTES-COSTA, Fernanda Magalhaes; MARTINS, Milton Arruda; DUARTE, Alberto Jose da Silva; SATO, Maria NotomiMaternal immunization with allergens, such as ovalbumin (OVA), can inhibit the development of an allergic response in offspring. The regulatory mechanisms seem to be mediated by maternal antibodies (MatAbs) and factors generated by the maternal fetal interface. The aim of this study was to verify the pathways of inhibitory Ab transference after maternal immunization with OVA and the effect of the offspring's dendritic cells (DCs) on the generation of regulatory T (Treg) cells. We verified that preconceptional OVA immunization induces high levels of proinflammatory and regulatory cytokines in the amniotic fluid, allowing the transference of high levels of anti-OVA IgG1 Abs to the offspring. Using an adoptive nursing protocol, we verified that maternal immunization leads to MatAb transference by the placental route and by breastfeeding contribute to the inhibition of anaphylactic IgE and IgG1 Ab responses in immunized offspring. We observed that maternal immunization decreased eosinophil numbers in recovered bronchoalveolar lavage fluid and in the lung tissue, whereas with a lack of control of airway responsiveness to methacholine. Maternal immunization induced in young offspring a decreased percentage of CD11c+ DCs expressing MHC class II and CD40 molecules. Moreover, DCs from both groups of offspring when pulsed with OVA, were able to induce Treg cells in vitro. Similarly, OVA immunization at the neonatal stage increased the frequency of Treg cells, regardless of the mother's immunization status. These findings emphasize that maternal immunization leads to a complex interaction of regulatory factors, with MatAbs, DCs and Treg cells affecting the tolerance of offspring during an allergic response.
conferenceObject Bordetella Pertussis Whole-Cell Vaccine Inhibits Specific IgE, Inflammation and Airway Remodeling in a Murine Model of Asthma(2015) AUN, Marcelo Vivolo; ARANTES-COSTA, Fernanda; SARAIVA-ROMANHOLO, Beatriz Mangueira; ALMEIDA, Francine Maria; REGINA-BRUEGGERMANN, Thayse; MARTINS, Milton Arruda; KALIL, Jorge; GIAVINA-BIANCHI, Pedro- iNOS Inhibition Reduces Lung Mechanical Alterations and Remodeling Induced by Particulate Matter in Mice(2019) PRADO, Carla Maximo; RIGHETTI, Renato Fraga; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; LEICK, Edna Aparecida; ARANTES-COSTA, Fernanda Magalhaes; ALMEIDA, Francine Maria de; SALDIVA, Paulo Hilario Nascimento; MAUAD, Thais; TIBERIO, Iolanda de Fatima Lopes Calvo; MARTINS, Milton de ArrudaBackground. The epidemiologic association between pulmonary exposure to ambient particulate matter (PM) and acute lung damage is well known. However, the mechanism involved in the effects of repeated exposures of PM in the lung injury is poorly documented. This study tested the hypotheses that chronic nasal instillation of residual oil fly ash (ROFA) induced not only distal lung and airway inflammation but also remodeling. In addition, we evaluated the effects of inducible nitric oxide inhibition in these responses. For this purpose, airway and lung parenchyma were evaluated by quantitative analysis of collagen and elastic fibers, immunohistochemistry for macrophages, neutrophils, inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and alveolar septa 8-iso prostaglandin F2 (8-iso-PGF-2) detection. Anesthetized in vivo (airway resistance, elastance, H, G, and Raw) respiratory mechanics were also analyzed. C57BL6 mice received daily 60ul of ROFA (intranasal) for five (ROFA-5d) or fifteen days (ROFA-15d). Controls have received saline (SAL). Part of the animals has received 1400W (SAL+1400W and ROFA-15d+1400W), an iNOS inhibitor, for four days before the end of the protocol. A marked neutrophil and macrophage infiltration and an increase in the iNOS, nNOS, and 8-iso-PGF2 expression was observed in peribronchiolar and alveolar wall both in ROFA-5d and in ROFA-15d groups. There was an increment of the collagen and elastic fibers in alveolar and airway walls in ROFA-15d group. The iNOS inhibition reduced all alterations induced by ROFA, except for the 8-iso-PGF2 expression. In conclusion, repeated particulate matter exposures induce extracellular matrix remodeling of airway and alveolar walls, which could contribute to the pulmonary mechanical changes observed. The mechanism involved is, at least, dependent on the inducible nitric oxide activation.
conferenceObject A compare between two models of cigare tie smoke co-exposition in ovalbumin-sensitized mice(2014) BRUGGEMANN, Thayse Regina; FERNANDES, Paula; OLIVEIRA, Jessica Marques Saez; SARAIVA-ROMANHOLO, Beatriz Mangueira; MARTINS, Milton Arruda; ARANTES-COSTA, Fernanda Magalhaes