LUZIA NAOKO SHINOHARA FURUKAWA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina
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- Exposure to fine particulate matter in the air alters placental structure and the renin-angiotensin system(2017) SOTO, Sonia de Fatima; MELO, Juliana Oliveira de; MARCHESI, Guilherme D'Aprile; LOPES, Karen Lucasechi; VERAS, Mariana Matera; OLIVEIRA, Ivone Braga de; SOUZA, Regiane Machado de; CASTRO, Isac de; FURUKAWA, Luzia Naoko Shinohara; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel C.Transforming growth factor beta 1 (TGF beta 1), the uteroplacental renin-angiotensin system (RAS) and vascular endothelial growth factor A (VEGF-A) participate in the placentation process. The aim of this study was to investigate the effects of exposure to pollutants on the placenta. Methods Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning on day 6 of pregnancy. At embryonic day 19, the placenta was collected. The placental structure, the protein and gene expression of TGF beta 1, VEGF-A, and its receptor Flk-1 and RAS were evaluated by indirect ELISA and quantitative real-time PCR. Results Exposure to P decreased the placental mass, size, and surface area as well as the TGF beta 1, VEGF-A and Flk-1 content. In the maternal portion of the placenta, angiotensin II (AngII) and its receptors AT(1) (AT(1)R) and AT(2) (AT(2)R) were decreased in the PF and PP groups. In the fetal portion of the placenta, AngII in the FP, PF and PP groups and AT(2)R in the PF and PP groups were decreased, but AT(1)R was increased in the FP group. VEGF-A gene expression was lower in the PP group than in the FF group. Conclusions Exposure to pollutants before and/or during pregnancy alters some characteristics of the placenta, indicating a possible impairment of trophoblast invasion and placental angiogenesis with possible consequences for the maternal-fetal interaction, such as a limitation of fetal nutrition and growth.
conferenceObject HIGH-SALT INTAKE DURING PREGNANCY MODIFIES PLACENTA PHENOTYPE AT LEAST UNTIL THE THIRD GENERATION(2014) PERES, Maria A.; SOTO, Sonia F.; GUIMARADES, Carina F.; SILVA, Rafaela C. P.; OLIVEIRA, Ivone B. de; FURUKAWA, Luzia N. S.; HEIMANN, Joel C.- The effects of individually ventilated cages on the respiratory systems of male and female Wistar rats from birth until adulthood(2017) MARCHESI, Guilherme DAprile; SOTO, Sonia de Fatima; CASTRO, Isac de; RODRIGUES, Thiago Guimaraes; MORIYA, Henrique Takachi; ALMEIDA, Francine Maria de; PAZETTI, Rogerio; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: To evaluate the respiratory systems of male and female rats maintained in individually ventilated cages (IVCs) from birth until adulthood. METHODS : Female Wistar rats were housed in individually ventilated cages or conventional cages (CCs) and mated with male Wistar rats. After birth and weaning, the male offspring were separated from the females and kept in cages of the same type until 12 weeks of age. RESULTS: The level of food consumption was lower in male offspring (IVC=171.7 +/- 9; CC=193.1 +/- 20) than in female offspring (IVC=100.6 +/- 7; CC=123.4 +/- 0.4), whereas the water intake was higher in female offspring (IVC=149.8 +/- 11; CC=99.2 +/- 0) than in male offspring (IVC=302.5 +/- 25; CC=249.7 +/- 22) at 11 weeks of age when housed in IVCs. The cage temperature was higher in individually ventilated cages than in conventional cages for both male (IVCs=25.9 +/- 0.5; CCs=22.95 +/- 0.3) and female (IVCs=26.2 +/- 0.3; CCs=23.1 +/- 0.3) offspring. The respiratory resistance (IVC=68.8 +/- 2.8; CC=50.6 +/- 3.0) and elastance (IVC=42.0 +/- 3.9; CC=32.4 +/- 2.0) at 300 mm/kg were higher in the female offspring housed in ventilated cages. The ciliary beat values were lower in both the male (IVCs=13.4 +/- 0.2; CC=15 +/- 0.4) and female (IVC=13.5 +/- 0.4; CC=15.9 +/- 0.6) offspring housed in individually ventilated cages than in those housed in conventional cages. The total cell (IVC=117.5 +/- 9.7; CC=285.0 +/- 22.8), neutrophil (IVC=13.1 +/- 4.8; CC=75.6 +/- 4.1) and macrophage (IVC=95.2 +/- 11.8; CC=170.0 +/- 18.8) counts in the bronchoalveolar lavage fluid were lower in the female offspring housed in individually ventilated cages than in those housed in conventional cages. CONCLUSIONS : The environmental conditions that exist in individually ventilated cages should be considered when interpreting the results of studies involving laboratory animals. In this study, we observed gender dimorphism in both the water consumption and respiratory mechanics of rats kept in ventilated cages.
- Inhalation of fine particulate matter during pregnancy increased IL-4 cytokine levels in the fetal portion of the placenta(2015) MELO, Juliana Oliveira de; SOTO, Sonia Fatima; KATAYAMA, Isis Akemi; WENCESLAU, Camilla Ferreira; PIRES, Amanda Gonalves; VERAS, Mariana Matera; FURUKAWA, Luzia N. S.; CASTRO, Isac de; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel ClaudioThis study aimed to verify the development of placental and systemic inflammation in rats exposed to fine particulate matter before or during pregnancy. Wistar rats were exposed to filtered air ( control) or to a load of 600 mu g/m(3) of fine particles in the air. The gene expression of IL-1 beta, IL-4, IL-6, IL-10, INF-gamma, TNF-alpha and Toll-like receptor 4 in the placenta was evaluated. The serum and placental concentrations of IL-1 beta, IL-4, IL-6, IL-10, INF-gamma and TNF-alpha were measured. The total and differential blood leukocyte and blood platelet count was assessed. Compared to control animals, IL-4 content was elevated in the fetal portion of the placenta in rats exposed to air pollution before and during pregnancy. Increased IL-4 suggests that a placental inflammatory reaction may have occurred in response to exposure to fine particulate matter and that this cytokine was responsible, among possibly others factors, for resolution of the inflammatory reaction.