FERNANDA MARCIANO CONSOLIM COLOMBO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats(2017) FERIANI, Daniele J.; SOUZA, Gabriel I. H.; CARROZZI, Nicolle M.; MOSTARDA, Cristiano; DOURADO, Paulo M. M.; CONSOLIM-COLOMBO, Fernanda M.; ANGELIS, Katia De; MORENO, Heitor; IRIGOYEN, Maria Claudia; RODRIGUES, BrunoBackground: The effects of exercise training (ET) associated with pyridostigmine bromide (PYR) treatment on cardiac and autonomic function, as well as on inflammatory profile after myocardial infarction (MI), are unclear. Methods: Male Wistar rats were randomly assigned to: control (C); sedentary + infarcted (I); sedentary + infarcted treated with PYR (IP); infarcted submitted to aerobic exercise training (IT); and infarcted submitted to treatment with PYR and aerobic exercise training (ITP). After 12 weeks of ET (50-70% maximal running speed; 1 h a day, 5 days a week) and/or PYR treatment (0.14 mg/mL on drink water), hemodynamic, autonomic and cytokines expression were performed. Results: Weobserved that both aerobic ET, associated or not with PYR treatment in MI animals, were able to: reduced MI area, improved systolic and diastolic function, baroreflex sensitivity, cardiovascular autonomic modulation, and tonic activity of the sympathetic and parasympathetic nervous system. Also, they led to a reduction of inflammatory profile measured at plasma, left ventricle and soleus skeletal muscle. However, additional effects were observed when ET and PYR were associated, such as an increase in vagal tonus and modulation, reduction of MI area, interferon-gamma and tumor necrosis factor-alpha (TNF-alpha), as well as an increase of interleukin-10/TNF-alpha ratio on left ventricle. Conclusion: These data suggest that associating ET and PYR promotes some additional benefits on cardiovascular autonomic modulation and inflammatory profile in infarcted rats.
- Galantamine alleviates inflammation and insulin resistance in patients with metabolic syndrome in a randomized trial(2017) CONSOLIM-COLOMBO, Fernanda M.; SANGALETI, Carine T.; COSTA, Fernando O.; MORAIS, Tercio L.; LOPES, Heno F.; MOTTA, Josiane M.; IRIGOYEN, Maria C.; BORTOLOTO, Luiz A.; ROCHITTE, Carlos Eduardo; HARRIS, Yael Tobi; SATAPATHY, Sanjaya K.; OLOFSSON, Peder S.; AKERMAN, Meredith; CHAVAN, Sangeeta S.; MACKAY, Meggan; BARNABY, Douglas P.; LESSER, Martin L.; ROTH, Jesse; TRACEY, Kevin J.; PAVLOV, Valentin A.BACKGROUND. Metabolic syndrome (MetS) is an obesity-driven condition of pandemic proportions that increases the risk of type 2 diabetes and cardiovascular disease. Pathophysiological mechanisms are poorly understood, though inflammation has been implicated in MetS pathogenesis. The aim of this study was to assess the effects of galantamine, a centrally acting acetylcholinesterase inhibitor with antiinflammatory properties, on markers of inflammation implicated in insulin resistance and cardiovascular risk, and other metabolic and cardiovascular indices in subjects with MetS. METHODS. In this randomized, double-blind, placebo-controlled trial, subjects with MetS (30 per group) received oral galantamine 8 mg daily for 4 weeks, followed by 16 mg daily for 8 weeks or placebo. The primary outcome was inflammation assessed through plasma levels of cytokines and adipokines associated with MetS. Secondary endpoints included body weight, fat tissue depots, plasma glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), cholesterol (total, HDL, LDL), triglycerides, BP, heart rate, and heart rate variability (HRV). RESULTS. Galantamine resulted in lower plasma levels of proinflammatory molecules TNF (-2.57 pg/ml [95% CI -4.96 to -0.19]; P = 0.035) and leptin (-12.02 ng/ml [95% CI -17.71 to -6.33]; P < 0.0001), and higher levels of the antiinflammatory molecules adiponectin (2.71 mu g/ml [95% CI 1.93 to 3.49]; P < 0.0001) and IL-10 (1.32 pg/ml, [95% CI 0.29 to 2.38]; P = 0.002) as compared with placebo. Galantamine also significantly lowered plasma insulin and HOMA-IR values, and altered HRV. CONCLUSION. Low-dose galantamine alleviates inflammation and insulin resistance in MetS subjects. These findings support further study of galantamine in MetS therapy.
conferenceObject Influence of Body Fat Distribution in Autonomic Dysfunction of Young Obese Women(2017) VICENTE, Wanda Rafaela Pinto Lopes; FONSECA, Felipe Xerez Cepeda; SILVA, Leslie Virmondes da; HUSSID, Maria Fernanda; CORSO, Simone Dal; ANGELIS, Katia De; VIEIRA, Rodolfo de Paula; LOPES, Heno Ferreira; CONSOLIM-COLOMBO, Fernanda Marciano; TINUCCI, Tais; TROMBETTA, Ivani Credidio- The Role of Acute Intermittent Hypoxia in Neutrophil-Generated Superoxide, Sympathovagal Balance, and Vascular Functionin Healthy Subjects(2017) ALMEIDA, Germana P. L.; TROMBETTA, Ivani C.; CEPEDA, Felipe X.; HATANAKA, Elaine; CURI, Rui; MOSTARDA, Cristiano; IRIGOYEN, Maria C.; BARRETO-FILHO, Jose A. S.; KRIEGER, Eduardo M.; CONSOLIM-COLOMBO, Fernanda M.Introduction: Recurrent hypoxia (HPX), a hallmark of the obstructive sleep apnea (OSA), impairs autonomic balance, and increases arterial blood pressure (BP). Oxidative stress is one of the mechanisms involved in these alterations. The cumulative effect of acute intermittent HPX and the chronicity may determine whether the response crosses the threshold from having protective value to pathology. However, the impact of acute intermittent HPXreoxygenation on markers of oxidative stress in healthy individuals remains to be fully understood. Objective: To analyze the effects of the acute intermittent HPX on the generation of neutrophil-derived superoxide, sympathovagal balance, and vascular function in healthy subjects. Methods: We applied six cycles of intermittent HPX (10% O-2 and 90% N2) for 5 min followed by 2 min of room-air in 15 healthy volunteers (34 +/- 2 years; 22.3 +/- 0.46 kg/m(2)), without OSA (polysomnography), during wakefulness. During the experimental protocol, we recorded O-2 saturation, end-tidal CO2, heart rate (HR), systolic, and diastolic BP, cardiac output (CO) and peripheral resistance (PR). Cardiac sympathovagal balance was determined by HR variability analysis (low frequency and high frequency bands, LF/HF). Superoxide generation in polymorphonuclear neutrophil cells were established using relative luminescence units (PMNs RLU) at baseline (pre-HPX) and immediately after hypoxia induction (post-HPX6). we recorded O-2 saturation, end-tidal CO2, heart rate (HR), systolic, and diastolic BP, cardiac output (CO) and peripheral resistance (PR). Cardiac sympathovagal balance was determined by HR variability analysis (low frequency and high frequency bands, LF/HF). Superoxide generation in polymorphonuclear neutrophil cells were established using relative luminescence units (PMNs RLU) at baseline (pre-HPX) and immediately after hypoxia induction (post-HPX6). Results: The studied subjects had normal levels of BP, plasma glucose, lipid profile, and inflammatory marker (C-reactive protein). Acute intermittent HPX increased HR, systolic BP, CO, and decreased PR. Additionally, acute intermittent HPX increased PMNs RLU, measured post-HPX6 (470 +/- 50 vs. 741 +/- 135, P < 0.05). We found a similar increase in LF/HF post-HPX6 (0.91 +/- 0.11 vs. 2.85 +/- 0.40, P < 0.05). PR was diminished from pre-HPX to post-HPX6 (1.0 +/- 0.03 vs. 0.85 +/- 0.06, P < 0.05). Further analysis showed significant association between O-2 saturation and PMNs RLU (R = -0.62, P = 0.02), and with LF/HF (R = -0.79, P = 0.02) post-HPX6. In addition, an association was found between PMNs RLU and PR post-HPX6 (R = 0.58, P = 0.04). Conclusion: Acute exposure to intermittent HPX not only increased superoxide generation in neutrophils, but also impaired cardiac sympathovagal balance in healthy subjects. These data reinforce the role of intermittent HPX in superoxide generation on neutrophils, which may lead to an impairment in peripheral vascular resistance.
- Diabetes and cardiovascular events in high-risk patients: Insights from a multicenter registry in a middle-income country(2017) SCHAAN, Beatriz D.; FIGUEIREDO NETO, Jose Albuquerque de; MOREIRA, Leila Beltrami; LEDUR, Priscila; MATTOS, Luiz Alberto P.; MAGNONI, Daniel; PRECOMA, Dalton Bertolim; MACHADO, Carlos Alberto; BRASILEIRO, Antonio Luiz da Silva; PENA, Felipe Montes; HARZHEIM, Erno; MONTENEGRO, Sergio; BERNARDEZ-PEREIRA, Sabrina; DAMIANI, Lucas P.; CONSOLIM-COLOMBO, Fernanda M.; PAOLA, Angelo Amato Vicenzo de; ANDRADE, Jadelson; GUIMARAES, Jorge Ilha; BERWANGER, OtavioAims: The aim of this study was to determine the rate of major clinical events and its determinants in patients with previous cardiovascular event or not, and with or without diabetes from a middle-income country. Methods: REACT study is a multicenter registry conducted between July 2010 and May 2013 in Brazil. Patients were eligible if they were over 45 years old and high cardiovascular risk. Patients were followed for 12 months; data were collected regarding adherence to evidence-based therapies and occurrence of clinical events (all-cause mortality, non-fatal cardiac arrest, myocardial infarction, or stroke). Results: A total of 5006 subjects was included and analyzed in four groups: No diabetes and no previous cardiovascular event, n = 430; diabetes and no previous cardiovascular event, n = 1138; no diabetes and previous cardiovascular event, n = 1747; and diabetes and previous cardiovascular event, n = 1691. Major clinical events in one-year follow-up occurred in 332 patients. A previous cardiovascular event was associated with a higher risk of having another event in the follow-up (HR 2.31 95% CI 1.74-3.05, p < 0.001), as did the presence of diabetes (HR 1.28 95% CI 1.10-1.73, p = 0.005). In patients with diabetes, failure to reach HbA1c targets was related to poorer event-free survival compared to patients with good metabolic control (HR 1.70 95% CI 1.01-2.84, p = 0.044). Conclusions: In Brazil, diabetes confers high risk for major clinical events, but this condition is not equivalent to having a previous cardiovascular event. Moreover, not so strict targets for HbA1c in patients with diabetes and previous cardiovascular events might be considered.
- Association of obstructive sleep apnea with arterial stiffness and nondipping blood pressure in patients with hypertension(2017) JENNER, Raimundo; FATURETO-BORGES, Fernanda; COSTA-HONG, Valeria; LOPES, Heno F.; TEIXEIRA, Sandra H.; MARUM, Elias; GIORGI, Dante A. M.; CONSOLIM-COLOMBO, Fernanda M.; BORTOLOTTO, Luiz A.; LORENZI-FILHO, Geraldo; KRIEGER, Eduardo M.; DRAGER, Luciano F.Whether sex influences the association of obstructive sleep apnea (OSA) with markers of cardiovascular risk in patients with hypertension is unknown. In this study, 95 hypertensive participants underwent carotid-femoral pulse wave velocity, 24-hour ambulatory blood pressure monitoring, echocardiogram, and polysomnography after a 30-day standardized treatment with hydrochlorothiazide plus enalapril or losartan. OSA was present in 52 patients. Compared with non-OSA patients, pulse wave velocity values were higher in the OSA group (men: 11.1 +/- 2.2 vs 12.7 +/- 2.4m/s, P=.04; women: 11.8 +/- 2.4 vs 13.2 +/- 2.2m/s, P=.03). The proportion of diastolic dysfunction was significant in men and women with OSA. Compared with non-OSA patients, nondipping systolic blood pressure in OSA was higher in men (14.3% vs 46.4%) and in women (41.4% vs 65.2%). OSA was independently associated with pulse wave velocity (=1.050; P=.025) and nondipping systolic blood pressure (odds ratio, 3.03; 95% confidence interval, 1.08-8.55; P=.035) in the regression analysis. In conclusion, OSA is independently associated with arterial stiffness and nondipping blood pressure in patients with hypertension regardless of sex.
- Cholinergic Stimulation Improves Oxidative Stress and Inflammation in Experimental Myocardial Infarction(2017) BEZERRA, Otavio C.; FRANCA, Cristiane Miranda; ROCHA, Juraci Aparecida; NEVES, Gizele A.; SOUZA, Pamella Ramona M.; GOMES, Mariana Teixeira; MALFITANO, Christiane; LOLEIRO, Tatiane C. Alba; DOURADO, Paulo Magno; LLESUY, Susana; ANGELIS, Katia de; IRIGOYEN, Maria Claudia C.; ULLOA, Luis; CONSOLIM-COLOMBO, Fernanda M.We previously reported that cholinergic stimulation with pyridostigmine (PY) induces anti-inflammatory cell recruitment soon after myocardial infarction (MI). In this study, we evaluated the anti-inflammatory effects of PY during the proliferative phase of cardiac repair by analyzing the infiltration of macrophages, Treg lymphocytes, oxidative stress and inflammatory cytokines. Wistar rats underwent control sham surgery or ligation of the left coronary artery and were randomly allocated to remain untreated (untreated infarcted group, I) or to receive PY (30 mg.kg(-1).day(-1)) in the supplied water (infarcted treated group, I + PY). Blood pressure and heart rate variability were registered at day 5 post-MI. The animals were euthanized 7 days after thoracotomy, when the hearts were removed and processed for immunohistochemistry (CD68, CD206, FOXP3), cytokines (IL-1 beta, IL-6, IL-10, TNF-alpha) and oxidative stress (superoxide dismutase, catalase, glutathione peroxidase, lipidic and protein peroxidation). PY treatment increased parasympathetic modulation, M2 macrophages and the antioxidant enzyme activity but reduced protein oxidation (carbonyls) and the concentration of IL-1 beta, IL-6, TNF-alpha and IL-10. Cholinergic stimulation induces parasympathetic neuro-immune modulation and anti-inflammatory cell enrollment as well as prevents oxidative stress and cytokine production after MI.