RENATA DE FREITAS SAITO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Galectin-3 sensitized melanoma cell lines to vemurafenib (PLX4032) induced cell death through prevention of autophagy(2018) BUSTOS, S. O.; PEREIRA, G. J. S.; SAITO, R. F.; GIL, C. D.; ZANATTA, D. B.; SMAILI, S. S.; CHAMMAS, R.Melanoma is a current worldwide problem, as its incidence is increasing. In the last years, several studies have shown that melanoma cells display high levels of autophagy, a self-degradative process that can promote survival leading to drug resistance. Consequently, autophagy regulation represents a challenge for cancer therapy. Herein, we showed that galectin-3 (Gal-3), a β-galactoside binding lectin which is often lost along melanoma progression, is a negative regulator of autophagy in melanoma cells. Our data demonstrated that Gal-3low/negative cells were more resistant to the inhibition of the activity of the cancer driver gene BRAFV600E by vemurafenib (PLX4032). Interestingly, in these cells, starvation caused further LC3-II accumulation in cells exposed to chloroquine, which inhibits the degradative step in autophagy. In addition, Gal-3 low/negative tumor cells accumulated more LC3-II than Gal-3 high tumor cells in vivo. Resistance of Gal-3low/negative cells was associated with increased production of superoxide and activation of the Endoplasmic Reticulum (ER) stress response, as evaluated by accumulation of GRP78. Pharmacological inhibition of autophagy with bafilomycin A reversed the relative resistance of Gal-3low/negative cells to vemurafenib treatment. Taken together, these results show that the autophagic flux is dependent on Gal-3 levels, which attenuate the prosurvival role of autophagy. © Bustos et al.
- Eeyarestatin I sensitizes melanoma cells to cisplatin-induced cell death(2018) SAITO, Renata de Freitas; OTAKE, Andreia Hanada; CORTEZ, Margarita M.; GILLIES, Robbert J.; CHAMMAS, Roger
conferenceObject Jarid1b protects from metformin-induced chemosensitization to cisplatin through p53 downregulation in NSCLC(2018) TORTELLI JR., Tharcisio Citrangulo; TAMURA, Rodrigo Esaki; TOMITAO, Michele Tatiana Pereira; BUSTOS, Silvina Odete; SAITO, Renata de Freitas; LEANDRINI, Sarah Milani de Moraes; JACOMASSI, Mayara D'Aurea; RIBEIRO JR., Ulysses; STRAUSS, Bryan Erik; CHAMMAS, RogerconferenceObject GD3 ganglioside-enriched extracellular vesicles stimulate melanocyte migration(2018) OTAKE, Andreia H.; DUARTE, Ana Paula M.; SAITO, Renata de Freitas; RAMOS, Alexandre F.; CHAMMAS, Roger