RICARDO DOS SANTOS SIMOES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Hyperprolactinemia modifies extracellular matrix components associated with collagen fibrillogenesis in harderian glands of non- and pregnant female mice(2023) ARAUJO, Ariadne S. L.; SIMOES, Manuel de J.; JR, Osvaldo P. Araujo; SIMOES, Ricardo S.; BARACAT, Edmund C.; NADER, Helena B.; JR, Jose M. Soares; GOMES, Regina C. T.The harderian gland (HG) is a gland located at the base of the nictating membrane and fills the inferomedial aspect of the orbit in rodents. It is under the influence of the hypothalamic-pituitary-gonadal axis and, because of its hormone receptors, it is a target tissue for prolactin (PRL) and sex steroid hormones (estrogen and progesterone). In humans and murine, the anterior surface of the eyes is protected by a tear film synthesized by glands associated with the eye. In order to understand the endocrine changes caused by hyperprolactinemia in the glands responsible for the formation of the tear film, we used an animal model with metoclopramide-induced hyperprolactinemia (HPRL). Given the evidences that HPRL can lead to a process of cell death and tissue fibrosis, the protein expression of small leucine-rich proteoglycans (SLRPs) was analyzed through immunohistochemistry in the HG of the non-and the pregnant female mice with hyperprolactinemia. The SRLPs are related to collagen fibrillogenesis and they participate in pro-apoptotic signals. Our data revealed that high prolactin levels and changes in steroid hormones (estrogen and progesterone) can lead to an alteration in the amount of collagen, and in the structure of type I and III collagen fibers through changes in the amounts of lumican and decorin, which are responsible for collagen fibrillogenesis. This fact can lead to the impaired functioning of the HG by excessive apoptosis in the HG of the non-and the pregnant female mice with HPRL and especially in the HG of pregnancy-associated hyperprolactinemia.
- May isoflavones prevent breast cancer risk?(2022) CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; SASSO, Gisela da Silva; VIEIRA, Renata Ramos; LIMA, Patricia Azevedo; SIMOES, Manuel de Jesus; JR, Jose Maria Soares
- Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats(2014) MAGANHIN, Carla C.; SIMOES, Ricardo S.; FUCHS, Luiz F. P.; SASSO, Gisela R. S.; SIMOES, Manuel J.; BARACAT, Edmund C.; SOARES JR., Jose M.Objective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Design: Experimental research. Setting: University research laboratory. Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis. Result(s): The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine.
conferenceObject Melatonin deficiency may decrease imnunoexpression of estrogen receptors in ovaries of pinealectomized female rats(2014) FLORENCIO-SILVA, Rinaldo; MAGANHIN, Carla C.; SASSO, Gisela R.; FRANCO, Paulo C.; SIMOES, Ricardo S.; SIMOES, Manuel J.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- Hormonal and Metabolic Factors Influence the Action of Progesterone on the Endometrium of Women with Polycystic Ovary Syndrome(2023) BARACAT, Maria Candida P.; BARACAT, Edmund C.; SIMOES, Ricardo S.; SIMOES, Manuel J.; MACIEL, Gustavo A. R.; AZZIZ, Ricardo; JR, Jose Maria SoaresHormonal and metabolic factors may influence endometrial quality and interfere with the action of progesterone. Therefore, the aim of our study was to address this issue. Participants were recruited from an outpatient reproductive endocrinology clinic at an academic tertiary medical care centre. All subjects underwent endometrial biopsy (EB) in the follicular phase of the cycle prior to treatment. Thereafter, they were treated with micronized progesterone (400 mg/day x 10 days intravaginally) from days 14-28 of the next cycle. A second EB was performed between days 21-24 of the cycle (the second phase). The metabolic and hormonal serum levels were evaluated during the implantation window. EB samples were analysed using light microscopy for histomorphometric analysis. The endometrium of women with Polycystic Ovarian Syndrome (PCOS) in the second phase demonstrated a uniform surface epithelium with less leukocyte infiltration and an absence of apoptotic figures compared to the control group. (p < 0.021). The thickness of the surface epithelium in the second phase of the PCOS group correlated positively with free and bioavailable testosterone values. The number of stromal cells increases with increasing insulin levels. Our results suggest that histomorphometric abnormalities of the endometrium persist and are linked to androgen and insulin levels despite progesterone supplementation in PCOS.
- Effects of estrogen and raloxifene on synaptic density in the hippocampal CA1 region of ovariectomized rats(2023) MENEZESDASILVA, Glaucia Mara; VEIGA, Eduardo Carvalho de Arruda; SIMOES, Manuel Jesus; SIMOES, Ricardo Santos; SHIROMA, Marcos Eiji; BARACAT, Maria Candida Pinheiro; CAVALCANTI, Givanna Santos; SOARES JUNIOR, Jose Maria; BARACAT, Edmund ChadaIntroduction: The CA1 region of the hippocampus has an important role in learning and memory. It has been shown that estrogen deficiency may reduce the synaptic density in the region and that hormone replacement ther-apy may attenuate the reduction. Objectives: This study aimed to evaluate the effects of estrogen and raloxifene on the synaptic density profile in the CA1 region of the hippocampus in ovariectomized rats.Methods: Sixty ovariectomized three-month-old virgin rats were randomized into six groups (n = 10). Treatments started either three days (early treatment) or sixty days (late treatment) after ovariectomy. The groups received propylene glycol vehicle (0.5 mL/animal/day), equine conjugated estrogens (50 mu g/animal/day), or raloxifene (3 mg/kg/day) either early or late after ovariectomy. The drugs were administered orally by gavage for 30 days. At the end of the treatments, the animals were anesthetized and transcardially perfused with ether and saline solu-tion. The brains were removed and prepared for analysis under transmission electron microscopy and later fixed. Results: Results showed a significant increase in the synaptic density profile of the hippocampal CA1 region in both the early estrogen (0.534 +/- 0.026 mu/m2) and the early raloxifene (0.437 +/- 0.012 mu/m2) treatment groups compared to the early or late vehicle-treated control groups (0.338 +/- 0.038 mu/m2 and 0.277 +/- 0.015 mu/m2 respectively).Conclusions: The present data suggest that the raloxifene effect may be lower than that of estrogen, even early or late treatment, on synaptic density in the hippocampus.