RICARDO DOS SANTOS SIMOES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
12 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 12
- Soybean isoflavones attenuate the expression of genes related to endometrial cancer risk(2015) CARBONEL, A. A. Ferraz; CALIO, M. Longoni; SANTOS, M. Aparecida; BERTONCINI, C. R. Antonio; SASSO, G. da Silva; SIMOES, R. Santos; SIMOES, M. Jesus; SOARES JR., J. M.Objective We evaluated whether genistein or estrogen treatment has the same effect when administered immediately or late to rats induced with menopause using ovariectomy. Methods Sixty adult female rats were divided into six treatment groups: GI = vehicle immediately after ovariectomy; GII = vehicle 30 days after ovariectomy; GIII = genistein immediately after ovariectomy; GIV = genistein 30 days after ovariectomy; GV = estrogen immediately after ovariectomy; and GVI = estrogen 30 days after ovariectomy. All animals were treated for 30 consecutive days. At the end of the treatment, part of the uteri was removed for subsequent histological studies and another part was used to evaluate estrogen receptors 1 and 2, cell proliferation (cyclin A1 and A2, cyclin D1, cyclin-dependent kinase inhibitors 1, 1B and 2, antigen identified by the monoclonal antibody Ki67) and angiogenesis (vascular endothelial growth factor, VEGF-A) gene expression. Results Late treatment after castration in rats resulted in more developed endometrium, enhanced cell proliferation and estrogen-signalling pathways, particularly the cyclin-related genes Ki67 and VEGF-A, compared to early treatment. Interestingly, these same effects were less intense with genistein compared to those induced by estrogen, especially when genistein was administered late. Conclusion Our data show that isoflavone renders a lower risk of cancer when compared to estrogen in treatments.
- Systematic review of cell adhesion molecules and estrogen receptor expression in the endometrium of patients with polycystic ovary syndrome(2015) BARACAT, Maria Candida P.; SERAFINI, Paulo C.; SIMOES, Ricardo dos S.; MACIEL, Gustavo A. R.; SOARES- JR., Jose M.; BARACAT, Edmund C.Background: Infertility associated with polycystic ovary syndrome (PCOS) could be related to many mechanisms including endometrial factors. Objectives: To review cell adhesion molecule and estrogen receptor expression in the endometrium. Search strategy: A systematic review was performed of the Medline and Cochrane databases for papers published in any language between 2004 and 2014. The search term was ""'polycystic ovary syndrome' OR 'Stein Leventhal syndrome' OR 'anovulation' AND 'endometrium' OR 'endometria."" Selection criteria: Research studies on endometrial cell adhesion molecules and estrogen receptor expression among women with PCOS diagnosed according to the Rotterdam criteria were included. Data collection and analysis: Data were extracted from identified studies and the quality of assessment was analyzed. Main results: Six studies were included. Data were controversial with respect to MUC1 and alpha V beta 3 integrin expression with significantly higher and lower levels, respectively, in women with PCOS. Estrogen receptor expression was enhanced among patients with PCOS as compared with healthy women. Conclusions: Endometrial factors influence embryo receptivity as indicated by the molecular mediators identified in the studies, including cell adhesion molecules and the estrogen receptor.
- Birth route in case of cesarean section in a previous pregnancy(2015) SIMOES, Ricardo; BERNARDO, Wanderley M.; SALOMAO, Antonio J.; BARACAT, Edmund C.
- Elevated serum osteoprotegerin levels in women: friend or foe?(2015) SASSO, Gisela Rodrigues da Silva; FLORENCIO-SILVA, Rinaldo; SIMOES, Ricardo Santos; BARACAT, Maria Candida Pinheiro; SOARES JUNIOR, Jose Maria; BARACAT, Edmund ChadaIntroduction: osteoprotegerin has emerged as a new candidate for the treatment of osteoporosis. However, high levels of osteoprotegerin have been linked to vascular calcification, an independent and well-defined risk factor for cardiovascular disease (CVD) and mortality. Thus, the action of osteoprotegerin in these situations has been questioned. Objective: to evaluate the effect of osteoprotegerin (OPG) on the human body, especially in bone tissue and in vascular diseases. Methods: the scientific databases consulted were PubMed-Medline and Cochrane, using keywords (MeSH terms) grouped into the following syntaxes: (Osteoprotegerin OR Osteoclastogenesis Inhibitory Factor OR Receptors, Tumor Necrosis Factor, Member 11b OR Tumor Necrosis Factor Receptor Superfamily, Member 11b OR FDCR-1 Protein OR FDCR 1 Protein OR OCIF Protein OR Follicular Dendritic Cell-Derived Receptor-1) AND (Bones AND Bone OR Bones AND Bone Tissue OR Bones OR Bone Tissue OR Cardiovascular Diseases). Results: Osteoprotegerin is present in various organs and binds to two ligands: nuclear factor kB (RANKL) related to the differentiation of osteoclasts, and tumor necrosis factor related to the apoptosis-inducing ligand (TRAIL). OPG inhibits the regulation effects of nuclear factor kB on inflammation and on the skeletal and vascular systems, preventing the apoptosis induced by TRAIL, being related to the preservation of bone tissue. Conclusion: a deeper knowledge of the mechanisms involved in the association between OPG serum levels, bone integrity and cardiovascular disease can provide important data for future therapeutic interventions.
- Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation(2015) LIMA, Gisele Negro; MAGANHIN, Carla Cristina; SIMOES, Ricardo Santos; BARACAT, Maria Candida Pinheiro; SASSO, Gisela Rodrigues da Silva; FUCHS, Luiz Fernando Portugal; SIMOES, Manuel de Jesus; BARACAT, Edmund Chada; SOARES JUNIOR, Jose MariaOBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 mu g/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip (R) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip (R) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis.
conferenceObject Imunoexpression of BCL-2 and BAX in ovarian follicles of pinealectomized or melatonin-treated pinealectomized rats(2015) FUCHS, Luiz F.; MAGANHIN, Carla C.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- The evaluation of endometrial sulfate glycosaminoglycans in women with polycystic ovary syndrome(2015) GIORDANO, Mario Vicente; GIORDANO, Luiz Augusto; GOMES, Regina Celia Teixeira; SIMOES, Ricardo Santos; NADER, Helena Bonciani; GIORDANO, Mario Gaspare; BARACAT, Edmund Chada; SOARES JUNIOR, Jose MariaThe aim of this study was to quantify the sulfated glycosaminoglycans in the endometria of women with polycystic ovary syndrome (PCOS). Of the 18 patients recruited for this study, 10 patients with PCOS comprised the PCOS group (PCOSG), and eight patients with regular and ovulatory menstrual cycles comprised the control group (CG). The clinical, biochemical, morphological and endometrial data from both groups were analyzed. Biopsies were performed during the proliferative phase of the menstrual cycle for the CG and during the persistent proliferative phase for the PCOSG (all women were amenorrheic). In the PCOSG, there was a significant increase in the endometrial concentration levels of heparan sulfate (p=0.03), but no difference in the concentrations of chondroitin sulfate was determined between the two groups (p=0.77). Period of time without menstruation (p=0.001) and body mass index (BMI) (p=0.04) correlated directly and positively with heparan sulfate concentration. There was no association between heparan sulfate levels and basal insulin values (p=0.08). High levels of endometrial heparan sulfate in women with PCOS indicate an interference with maternal-fetal recognition, which contributes to infertility; thus, endometrial heparan sulfate may be a predictive marker of future neoplasia risk.
conferenceObject Expression and influence ovarian function by the upregulation and downregulation of steroidogenesis related genes in ovary of pinealectomized rats in proestrous phase: Folistatin and Inhibin-beta A(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SIMOES, Ricardo S.; SASSO, Gisela R.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- Effects of metformin on the adrenal cortex of androgenized rats(2015) MACEDO, Lilian Alves; CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; FUCHS, Luiz Fernando Portugal; AMARAL, Vinicius Cestari do; SIMONCINI, Tommaso; SIMOES, Manuel Jesus Santos; BARACAT, Edmund Chada; SOARES JR., Jose MariaObjective: To evaluate the sex steroid profile and histomorphometry of the adrenal cortical zones of androgenized rats (wistar) with polycystic ovary syndrome treated with metformin. Study design: Thirty animals were divided into three groups: GC (regular estrous cycle), GPE (permanent estrus), and GPEM (permanent estrus + metformin 28 mg/kg for 50 days). At the end of this period, blood was collected for hormone measurement. The width of the adrenal cortical zones and the nuclear volumes were analyzed by histomorphometry. The ANOVA test was used in the statistical analysis. Results: The adrenal glands of the androgenized animals were larger and more intensely vascularized than those of the other groups. The concentration of androstenedione in GPE was higher than that in the other groups (0.4 +/- 0.1*>= 0.2 +/- 0.1 = 0.2 +/- 01, *p<50.05). The width of the zona glomerulosa and of the zona reticularis and their nuclear volumes were greater in GPE compared to those of the other groups (GPE*>GPEM = GC, *p<50.05). Conclusion: Metformin treatment may decrease the serum levels of androstenedione as well as the width and the nuclear volumes of the zona glomerulosa and of the zona reticularis in androgenized animals.
conferenceObject Imnunoexpression of estrogen receptors may decrease produce melatonin in ovaries of pinealectomized female rats(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES JUNIOR, Jose M.