FABIANA MARTINS DE PAULA
Projetos de Pesquisa
Unidades Organizacionais
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
12 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 12
- Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostics(2020) FONSECA, Priscilla D. M.; CORRAL, Marcelo A.; COSENZA-CONTRERAS, Miguel; MEISEL, Dirce M. C. L.; MELO, Gessica B.; ANTUNES, Milena M. S.; SANTO, Maria C. E.; GRYSCHEK, Ronaldo C. B.; COSTA-CRUZ, Julia M.; CASTRO-BORGES, William; PAULA, Fabiana M.Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We believe that the protein profile presented in this shotgun analysis extends our understanding of the protein composition within the Strongyloides genus, opening up new perspectives for research on biomarkers that may help with the diagnosis of human strongyloidiasis. Data are available via ProteomeXchange with identifier PXD013703.
- Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostic (vol 235, 111249, 2020)(2020) FONSECA, Priscilla D. M.; CORRAL, Marcelo A.; COSENZA-CONTRERAS, Miguel; MEISEL, Dirce M. C. L.; MELO, Gessica B.; ANTUNES, Milena M. S.; SANTO, Maria C. E.; GRYSCHEK, Ronaldo C. B.; COSTA-CRUZ, Julia M.; CASTRO-BORGES, William; PAULA, Fabiana M.
- Molecular diagnosis of Strongyloides stercoralis among transplant candidates(2018) PAULA, Fabiana M.; MALTA, Fernanda M.; MARQUES, Priscilla D.; MELO, Gessica B.; CORRAL, Marcelo A.; GOTTARDI, Maiara; PINHO, Joao R. R.; GONCALVES, Elenice M. N.; CASTILHO, Vera L. P.; PIERROTTI, Ligia C.; ABDALA, Edson; COSTA, Silvia F.; CHIEFFI, Pedro P.; GRYSCHEK, Ronaldo C. B.Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S.stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S.stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S.stercoralis infection among transplant candidates.
- Screening of Strongyloides infection using an ELISA test in transplant candidates(2019) TOLEDO, Beatriz; CORRAL, Marcelo A.; MEISEL, Dirce Mary C. L.; GOTTARDI, Maiara; ABDALA, Edson; COSTA, Silvia F.; PIERROTTI, Ligia Camera; LESCANO, Susana A. Z.; GONCALVES, Elenice M. N.; CASTILHO, Vera L. P.; CHIEFFI, Pedro P.; GRYSCHEK, Ronaldo C. B.; PAULA, Fabiana M.OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.
conferenceObject GENETIC DIVERSITY OF BLASTOCYSTIS SUBTYPES IN PATIENTS WITH CHRONIC URTICARIA(2017) PAULA, Fabiana M.; MELO, Gessica B.; MALTA, Fernanda M.; MARUTA, Celina W.; CRIADO, Paulo R.; CASTILHO, Vera Lucia P.; GONCALVES, Elenice M. N.; SANTO, Maria Cristina Espirito; GRYSCHEK, Ronaldo CesarconferenceObject DIFFERENTIAL EXPRESSION OF MEMBRANE AND MEMBRANE-BOUND PROTEINS FROM FILARIFORM LARVAE AND ADULT FEMALE OF STRONGYLOIDES VENEZUELENSIS(2017) PAULA, Fabiana M.; CORRAL, Marcelo A.; MARQUES, Priscilla D.; MEISEL, Dirce Mary C.; COSTA-CRUZ, Julia Maria; SANTO, Maria Cristina Espirito; CAMPOS, Jonatan M.; MATTEI, Bruno; BORGES, William Castro; GRYSCHEK, Ronaldo Cesar- Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients(2015) GOTTARDI, Maiara; PAULA, Fabiana M.; CORRAL, Marcelo A.; MEISEL, Dirce Mary C.; COSTA, Silvia F.; ABDALA, Edson; PIERROTTI, Ligia C.; YAMASHIRO, Juliana; CHIEFFI, Pedro Paulo; GRYSCHEK, Ronaldo Cesar B.Background: Strongyloides stercoralis is a parasite that causes human strongyloidiasis. The disease ranges from asymptomatic to severe forms, which are often fatal in immunocompromised individuals. Laboratory diagnosis is challenging owing to limitations in the use of conventional parasitological techniques. The present study aimed to evaluate the indirect immunofluorescence assay (IFA) using infective larvae of S. venezuelensis as an antigen for the immunodiagnosis of human strongyloidiasis in immunocompromised patients. Methods: Serum and stool samples from 200 immunocompromised patients (HIV-positive, HTLV-1-positive, and renal, liver, and/or bone marrow transplantation candidates) were used. Stool samples were examined using three parasitological methods: Lutz, Rugai, and culture agar plate. IFA was performed using sections of infective larvae of S. venezuelensis as antigens, and showed 95.4% sensitivity and 95.8% and specificity. Results: Among the 200 patients, 17 (8.5%) were positive for S. stercoralis by at least one parasitological method, and 43 (21.5%) were positive by IFA. Conclusions: IFA can be used as a screening method for the detection of S. stercoralis in immunocompromised patients.
conferenceObject IMMUNODIAGNOSIS OF STRONGYLOIDES STERCORALIS INFECTION IN CANDIDATE PATIENTS FOR TRANSPLANTATION(2015) GOTTARDI, Maiara; PAULA, Fabiana M.; CORRAL, Marcelo A.; MEISEL, Dirce M.; COSTA, Silvia F.; ABDALA, Edson; PIERROTTI, Ligia; CHIEFFI, Pedro Paulo; GRYSCHEK, Ronaldo C.- Molecular and Immnune Diagnosis: Further Testing for Human Strongyloidiasis(2018) BOSQUI, Larissa R.; MARQUES, Priscilla D.; MELO, Gessica B. de; GONCALVES-PIRES, Maria do Rosario F.; MALTA, Fernanda M.; PAVANELLI, Wander R.; CONCHON-COSTA, Ivete; COSTA-CRUZ, Julia M.; PAULA, Fabiana M.; COSTA, Idessania N.Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests. Stool samples were processed via parasitological methods. Serum samples were used for immunoglobulin G (IgG) detection and saliva samples for IgA detection by ELISA. For amplification by conventional PCR, two different primers were used: species specific (101 bp) and genus specific (392 bp). The results showed that 34 (97.1%) of the 35 copro-positive individuals for S. stercoralis were positive for serum IgG and 19 (54.3%) were positive for salivary IgA. Regarding molecular analysis, both primers (species and genus specific) demonstrated positivity in 100% of the samples, which was confirmed by sequencing the positive samples. Complementary examinations of the parasitological method demonstrated excellent results in the context of the diagnosis of strongyloidiasis, especially in asymptomatic patients with irregular larval release in the feces.
- Subtypes of Blastocystis sp. isolated in fecal samples from transplant candidates in Sao Paulo, Brazil(2020) SILVA, Maria do Rosario A.; MELO, Gessica B.; MALTA, Fernanda M.; ABDALA, Edson; COSTA, Silvia F.; PIERROTTI, Ligia C.; GONCALVES, Elenice M. N.; CASTILHO, Vera L. P.; CHIEFFI, Pedro P.; GRYSCHEK, Ronaldo C. B.; PAULA, Fabiana M.Blastocystis sp. is an intestinal protozoan commonly found in fecal samples of many animal species, including humans, but poorly studied in transplant candidates. The aim of this study was to evaluate the occurrence and molecular identification of Blastocystis sp. in fecal samples from transplant candidates. A polymerase chain reaction was performed using specific primers for Blastocystis ribosomal DNA. The DNA sequences obtained were aligned and compared with other sequences from the GenBank and MLST databases. The analyzed samples showed a positivity of 16% (24 of 150) for Blastocystis sp. The highest occurrence was observed in renal transplant candidates (31.4%), followed by hepatic transplant candidates (10.4%) and candidates for bone marrow transplantation (5.9%). Subtype (ST) 3 (45.8%) was the most prevalent among the isolates, followed by ST1 (375%), ST2 (12.5%), and ST7 (4.2%). This is the first study of molecular identification Blastocystis sp. in transplant candidates. Our results confirmed that ST3 was the most common subtype in transplant candidates and reinforce the importance of new studies to investigate of Blastocystis sp. in these patients. (C) 2019 Published by Elsevier Ltd on behalf of World Federation of Parasitologists.