VINICIUS DAGUANO GASTALDI

(Fonte: Lattes)
Índice h a partir de 2011
3
Lattes
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Psiquiatria, Hospital das Clínicas, Faculdade de Medicina
LIM/23 - Laboratório de Psicopatologia e Terapêutica Psiquiátrica, Hospital das Clínicas, Faculdade de Medicina

Filtros

Limpar filtros

Configurações

Tipo de acesso: restrictedAccess ×

Resultados de Busca

Agora exibindo 1 - 2 de 2
  • article 25 Citação(ões) na Scopus
    C-4 and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation
    (2020) FERRARI, Renata C.; BITTENCOURT, Priscila P.; RODRIGUES, Maria A.; MORENO-VILLENA, Jose J.; ALVES, Frederico R. R.; GASTALDI, Vinicius D.; BOXALL, Susanna F.; DEVER, Louisa V.; DEMARCO, Diego; ANDRADE, Sonia C. S.; EDWARDS, Erika J.; HARTWELL, James; FRESCHI, Luciano
    Although biochemically related, C-4 and crassulacean acid metabolism (CAM) systems are expected to be incompatible. However, Portulaca species, including P. oleracea, operate C-4 and CAM within a single leaf, and the mechanisms behind this unique photosynthetic arrangement remain largely unknown. Here, we employed RNA-seq to identify candidate genes involved exclusively or shared by C-4 or CAM, and provided an in-depth characterization of their transcript abundance patterns during the drought-induced photosynthetic transitions in P. oleracea. Data revealed fewer candidate CAM-specific genes than those recruited to function in C-4. The putative CAM-specific genes were predominantly involved in night-time primary carboxylation reactions and malate movement across the tonoplast. Analysis of gene transcript-abundance regulation and photosynthetic physiology indicated that C-4 and CAM coexist within a single P. oleracea leaf under mild drought conditions. Developmental and environmental cues were shown to regulate CAM expression in stems, whereas the shift from C-4 to C-4-CAM hybrid photosynthesis in leaves was strictly under environmental control. Moreover, efficient starch turnover was identified as part of the metabolic adjustments required for CAM operation in both organs. These findings provide insights into C-4/CAM connectivity and compatibility, contributing to a deeper understanding of alternative ways to engineer CAM into C-4 crop species.
  • article 3 Citação(ões) na Scopus
    DNA methylation mediates a randomized controlled trial home-visiting intervention during pregnancy and the Bayley infant's cognitive scores at 12 months of age
    (2022) EUCLYDES, Veronica L. V.; GASTALDI, Vinicius D.; FELTRIN, Arthur S.; HOFFMAN, Daniel J.; GOUVEIA, Gisele; COGO, Hugo; FELIPE-SILVA, Aloisio; VIEIRA, Rossana P.; MIGUEL, Euripedes C.; V, Guilherme Polanczyk; CHIESA, Anna; FRACOLLI, Lislaine; MATIJASEVICH, Alicia; FERRARO, Alexandre; ARGEU, Adriana; MASCHIETTO, Mariana; BRENTANI, Helena P.
    The crosstalk between maternal stress exposure and fetal development may be mediated by epigenetic mechanisms, including DNA methylation (DNAm). To address this matter, we collect 32 cord blood samples from low-income Brazilian pregnant adolescents participants of a pilot randomized clinical intervention study (ClinicalTrials.gov, Identifier: NCT02807818). We hypothesized that the association between the intervention and infant neurodevelopmental outcomes at 12 months of age would be mediated by DNAm. First, we searched genome methylation differences between cases and controls using different approaches, as well as differences in age acceleration (AA), represented by the difference of methylation age and birth age. According to an adjusted p-value <= 0.05 we identified 3090 differentially methylated positions- CpG sites (DMPs), 21 differentially methylated regions (DMRs) and one comethylated module weakly preserved between groups. The intervention group presented a smaller AA compared to the control group (p = 0.025). A logistic regression controlled by sex and with gestational age indicated a coefficient of -0.35 towards intervention group (p = 0.016) considering AA. A higher cognitive domain score from Bayley III scale was observed in the intervention group at 12 months of age. Then, we performed a potential causal mediation analysis selecting only DMPs highly associated with the cognitive domain (adj. R (2) > 0.4), DMRs and CpGs of hub genes from the weakly preserved comethylated module and epigenetic clock as raw values. DMPs in STXBP6, and PF4 DMR, mediated the association between the maternal intervention and the cognitive domain at 12 months of age. In conclusion, DNAm in different sites and regions mediated the association between intervention and cognitive outcome.