GERSON CHADI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Neurologia, Faculdade de Medicina - Docente
LIM/45 - Laboratório de Fisiopatologia Neurocirúrgica, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/45 - Laboratório de Fisiopatologia Neurocirúrgica, Hospital das Clínicas, Faculdade de Medicina - Líder
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bookPart Esclerose lateral amiotrófica e doenças do neurônio motor(2021) JORGE, Frederico Mennucci de Haidar; CHADI, Gerson- Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)(2015) ALVES, C. J.; GUIMARãES, A. B.; MAXIMINO, J. R.; BYDLOWSKI, S. P.; CHADI, G.Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations. © Springer Science+Business Media New York 2015. All rights are reserved.