CARLOS AUGUSTO GONCALVES PASQUALUCCI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
- Discrimination of Non-Melanoma Skin Lesions From Non-Tumor Human Skin Tissues In Vivo Using Raman Spectroscopy and Multivariate Statistics(2015) SILVEIRA, Fabricio L.; PACHECO, Marcos T. T.; BODANESE, Benito; PASQUALUCCI, Carlos A.; ZANGARO, Renato A.; SILVEIRA JR., LandulfoBackground and ObjectiveRaman spectroscopy was used to discriminate human non-melanoma skin lesions from non-tumor tissues in vivo. This work proposed the discrimination between non-melanoma (basal cell carcinoma, BCC; squamous cell carcinoma, SCC) and pre-cancerous lesions (actinic keratosis, AK) from benign lesions and normal (non-tumor group, NT) tissues, using near-infrared Raman spectroscopy with a Raman probe. Materials and MethodsPrior to surgery, the spectra of suspicious lesions were obtained in situ. The spectra of adjacent, clinically normal skin were also obtained. Lesions were resectioned and submitted for histopathology. The Raman spectra were measured using a Raman spectrometer (830nm). Two types of discrimination models were developed to distinguish the different histopathological groups. The principal components analysis discriminant analysis (PCA/DA) and the partial least squares discriminant analysis (PLS/DA) were based on Euclidean, quadratic and Mahalanobis distances. ResultsPCA and PLS spectral vectors showed spectral features of skin constituents, such as lipids (between 1,250cm(-1) and 1,300cm(-1) and at 1,450cm(-1)) and proteins (between 870cm(-1) and 940cm(-1), 1,240cm(-1) and 1,271cm(-1), and at 1,000cm(-1) and 1,450cm(-1)). Despite the small spectral differences between malignant lesions and benign tissues, the algorithms discriminated the spectra of non-melanoma skin and pre-cancerous lesions from benign and normal tissues, with an overall accuracy of 82.8% and 91.9%, respectively. ConclusionPCA and PLS could discriminate Raman spectra of skin tissues, opening the way for an in vivo optical diagnosis. Lasers Surg. Med. 47:6-16, 2015. (c) 2015 Wiley Periodicals, Inc.
conferenceObject D-serine levels in Alzheimer's disease: implications for novel biomarker development(2015) LOURENCO, M.; MADEIRA, C.; VARGAS-LOPES, C.; SUEMOTO, C.; BRANDAO, C. O.; REIS, T.; LEITE, R.; LAKS, J.; JACOB-FILHO, W.; PASQUALLUCCI, C.; GRINBERG, L.; FERREIRA, S.; PANIZZUTTI, R.conferenceObject Inflammation in the Perivascular Adipose Tissue is Associated With Coronary Artery Disease: An Autopsy Study(2015) FARIAS, Daniela S.; PASQUALUCCI, Carlos A.; NISHIZAWA, Aline; SILVA, Luiz F.; CAMPOS, Fernanda M.; SILVA, Karen C.; CUELHO, Anderson; LEITE, Renata E.; FERRETTI-REBUSTINI, Renata E.; GRINBERG, Lea T.; FARREL, Jose M.; JACOB-FILHO, Wilson; SUEMOTO, Claudia K.conferenceObject Human microglia transcriptome and cross-species analysis(2015) GALATRO, T. F. de A.; HOLTMAN, I. R.; BROUWER, N.; SOLA, P.; REIS, G. N.; VAINCHTEIN, I. D.; VERAS, M.; PEREIRA, T.; PASQUALUCCI, C.; SOGAYAR, M. C.; BODDEKE, E. W. G.; MARIE, S. K. N.; EGGEN, B. J. L.- Diabetes mellitus, alzheimer disease and vascular dementia: a clinicopathologic study(2015) MATIOLI, M. N. P. S.; SUEMOTO, C. K.; FARFEL, J. M.; FARIAS, D. S.; NEVES, R. C.; LEITE, R. E. P.; PASQUALUCCI, C. A.; JACOB FILHO, W.; GRINBERG, L. T.; NITRINI, R.
- Validity of the Katz Index to assess activities of daily living by informants in neuropathological studies(2015) FERRETTI-REBUSTINI, Renata Eloah de Lucena; BALBINOTTI, Marcos Alencar Abaide; JACOB-FILHO, Wilson; REBUSTINI, Flavio; SUEMOTO, Claudia Kimie; PASQUALUCCI, Carlos Augusto Goncalves; FARFEL, Jose Marcelo; LEITE, Renata Elaine Paraizo; GRINBERG, Lea Tenenholz; NITRINI, RicardoObjective: To analyze the evidences of construct validity of the Katz Index for the retrospective assessment of activities of daily living (ADL) by informants, to assist neuropathological studies in the elderly. Method: A cross-sectional study analyzed the functional ability of ADL measure by the Katz Index, of 650 cases randomly selected from the Brazilian Brain Bank of the Ageing Brain Study Group (BBBABSG) database. Sample was divided in two subsamples for the analysis (N=325, each) and then stratified according to cognitive decline assessed by the Clinical Dementia Rating Scale (CDR). Factor analyses with calculations of internal consistency and invariance were performed. Results: Factor analysis evidenced a unidimensional instrument with optimal internal consistency, in all subgroups. Goodness of fit indices were obtained after two treatments of covariance, indicating adequacy of the scale for assessing ADL by informants. The scale is invariant to cognitive decline meaning that it can be used for subjects with or without cognitive impairment. Conclusion: Katz Index is valid for the retrospective assessment of basic ADL by informants, with optimal reliability.
conferenceObject Argyrophilic grain disease may delay cognitive decline in AD: an autopsy study(2015) GRINBERG, Lea; RODRIGUEZ, Roberta; SUEMOTO, Claudia; MOLINA, Mariana; NASCIMENTO, Camila; LEITE, Renata; FERRETTI-REBUSTINI, Renata; FARFEL, Jose; HEINSEN, Helmut; NITRINI, Ricardo; PASQUALLUCCI, Carlos; JACOB-FILHO, Wilson; YAFFE, Kristine- Factors associated with morphometric brain changes in cognitively normal aging(2015) FERRETTI-REBUSTINI, Renata Eloah de Lucena; JACOB-FILHO, Wilson; SUEMOTO, Claudia Kimie; FARFEL, José Marcelo; LEITE, Renata Elaine Paraiso; GRINBERG, Lea Tenenholz; PASQUALUCCI, Carlos Augusto; NITRINI, RicardoOBJECTIVE: Cognitive impairment is associated with reductions in brain weight and volume. The factors related to morphometric brain changes in cognitively normal aging remain unknown. We aimed to identify which clinical factors are associated with morphometric brain changes in cognitively normal aging. METHODS: A cross-sectional study of 414 subjects, ≥50 years old submitted to clinical assessment and brain autopsy, after informed consent, was carried out at the São Paulo Autopsy Service, Brazil. Data on cognitive and functional evaluations were collected through structured interview applied to the next-of-kin. Brain weight (g) and volume (mL) measurements were obtained and adjusted for head circumference (cm). Associations between brain weight/volume and related factors were obtained through univariate and multivariate analysis. RESULTS: Participants were predominantly male (60.4%), Caucasian (69%), with mean age of 67.1 ± 10.9 years. Mean brain weight was 1219.2 ± 140.9 g, and mean brain volume was 1217.1 ± 152.3 mL. Head circumference was independently associated with low brain weight (p<0.001) and volume (p<0.001). Total and adjusted brain weight and volume decreased in some conditions. Female gender (p<0.001), hypertension (p<0.009), coronary artery disease (p<0.013) and walking assistance (p<0.011) were associated with lower adjusted brain weight while schooling was associated with higher adjusted brain weight (p<0.003). Female gender (p<0.001), age (p<0.001) and hypertension (p<0.011) were associated with low adjusted brain volume. CONCLUSION: Morphometric brain changes occur despite the absence of cognitive impairment and were predominantly associated with age, female gender, mobility impairment and cardiovascular conditions. Schooling may be a protective factor.
- D-serine levels in Alzheimer's disease: implications for novel biomarker development(2015) MADEIRA, C.; LOURENCO, M. V.; VARGAS-LOPES, C.; SUEMOTO, C. K.; BRANDAO, C. O.; REIS, T.; LEITE, R. E. P.; LAKS, J.; JACOB-FILHO, W.; PASQUALUCCI, C. A.; GRINBERG, L. T.; FERREIRA, S. T.; PANIZZUTTI, R.Alzheimer's disease (AD) is a severe neurodegenerative disorder still in search of effective methods of diagnosis. Altered levels of the NMDA receptor co-agonist, D-serine, have been associated with neurological disorders, including schizophrenia and epilepsy. However, whether D-serine levels are deregulated in AD remains elusive. Here, we first measured D-serine levels in post-mortem hippocampal and cortical samples from nondemented subjects (n = 8) and AD patients (n = 14). We next determined D-serine levels in experimental models of AD, including wild-type rats and mice that received intracerebroventricular injections of amyloid-beta oligomers, and APP/PS1 transgenic mice. Finally, we assessed D-serine levels in the cerebrospinal fluid (CSF) of 21 patients with a diagnosis of probable AD, as compared with patients with normal pressure hydrocephalus (n = 9), major depression (n = 9) and healthy controls (n = 10), and results were contrasted with CSF amyloid-beta/tau AD biomarkers. D-serine levels were higher in the hippocampus and parietal cortex of AD patients than in control subjects. Levels of both D-serine and serine racemase, the enzyme responsible for D-serine production, were elevated in experimental models of AD. Significantly, D-serine levels were higher in the CSF of probable AD patients than in non-cognitively impaired subject groups. Combining D-serine levels to the amyloid/tau index remarkably increased the sensitivity and specificity of diagnosis of probable AD in our cohort. Our results show that increased brain and CSF D-serine levels are associated with AD. CSF D-serine levels discriminated between nondemented and AD patients in our cohort and might constitute a novel candidate biomarker for early AD diagnosis.