CARLOS AUGUSTO GONCALVES PASQUALUCCI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
17 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 17
conferenceObject Brain transcriptome analysis of Japanese population living in Brazil(2019) MARIE, Suely Kazue Nagahashi; LERARIO, Antonio Marcondes; SHINJO, Sueli Mieko Oba; NASCIMENTO, Camila; LEITE, Renata; SUEMOTO, Claudia; PASQUALUCCI, Carlos Augusto; MURAYAMA, Shigeo- Direct Measurements of Abdominal Visceral Fat and Cognitive Impairment in Late Life: Findings From an Autopsy Study(2019) NISHIZAWA, Aline; CUELHO, Anderson; FARIAS-ITAO, Daniela S. de; CAMPOS, Fernanda M.; LEITE, Renata E. P.; FERRETTI-REBUSTINI, Renata E. L.; GRINBERG, Lea T.; NITRINI, Ricardo; JACOB-FILHO, Wilson; PASQUALUCCI, Carlos A.; SUEMOTO, Claudia K.Background: The relationship between cognitive impairment and abdominal visceral is controversial. Moreover, all studies so far used imaging studies to evaluate visceral fat and this association has not been described yet using autopsy material, which allows the direct quantification of abdominal fat. We aimed to investigate the association between direct measurements of abdominal visceral fat and cognitive impairment in an autopsy study. Methods: In this cross-sectional study, we collected information on sociodemographics, cardiovascular risk factors, and cognitive status from subjects aged 50 or older at time of death in a general autopsy service in Brazil. Abdominal visceral fat was obtained in natura by the dissection of perirenal, mesenteric, omental, and mesocolon fat. The associations of total abdominal visceral fat with cognitive impairment [clinical dementia rating (CDR) score >= 0.5] and CDR-sum of boxes (CDR-SB) were evaluated using logistic regression and negative binomial regression models, respectively. All analyses were adjusted for height, age, sex, education, hypertension, diabetes mellitus, stroke, smoking, alcohol use, and physical inactivity. In addition, we compared the discrimination of visceral fat, body mass index (BMI), and waist circumference (WC) measurements in predicting cognitive impairment. Results: We evaluated 234 participants (mean age = 71.2 +/- 12.9 years old, 59% male). Abdominal visceral fat was inversely associated with cognitive impairment (OR = 0.46, CI = 0.30; 0.70, p < 0.0001) and with CDR-SB scores (beta = 0.85, 95% CI = 1.28; 0.43, p < 0.0001). When we compared the area under the ROC curve (AUC), visceral fat (AUC = 0.754), BMI (AUC = 0.729), and WC (AUC = 0.720) showed similar discrimination in predicting cognitive impairment (p = 0.38). Conclusion: In an autopsy study, larger amount of directly measured abdominal visceral fat was associated with lower odds of cognitive impairment in older adults.
conferenceObject Polarized Macrophages in the Perivascular Adipose Tissue Were Correlated With Atherosclerotic Plaque Components in Coronary Arteries: An Autopsy Study(2019) FARIAS-ITAO, Daniela S.; PASQUALUCCI, Carlos A.; ANDRADE, Renato A.; SILVA, Luiz Fernando F.; CAMPO, Alexandre B.; SUEMOTO, Claudia K.- Is Olfactory Epithelium Biopsy Useful for Confirming Alzheimer's Disease?(2019) GODOY, Maria Dantas Costa Lima; FORNAZIERI, Marco Aurelio; DOTY, Richard L.; PINNA, Fabio de Rezende; FARFEL, Jose Marcelo; SANTOS, Glaucia Bento dos; MOLINA, Mariana; FERRETTI-REBUSTINI, Renata E. L.; LEITE, Renata E. P.; SUEMOTO, Claudia K.; GRINBERG, Lea T.; PASCRALUCCI, Carlos A. G.; VOEGELS, Richard Louis; NITRINI, Ricardo; JACOB FILHO, WilsonObjectives: The clinical symptoms of Alzheimer's disease (AD) are preceded by a long asymptomatic period associated with ""silent"" deposition of aberrant paired helical filament (PHF)-tau and amyloid-beta proteins in brain tissue. Similar depositions have been reported within the olfactory epithelium (OE), a tissue that can be biopsied in vivo. The degree to which such biopsies are useful in identifying AD is controversial. This postmortem study had 3 main goals: first, to quantify the relative densities of AD-related proteins in 3 regions of the olfactory neuroepithelium, namely, the nasal septum, middle turbinate, and superior turbinate; second, to establish whether such densities are correlated among these epithelial regions as well as with semi-quantitative ratings of general brain cortex pathology; and third, to evaluate correlations between the protein densities and measures of antemortem cognitive function. Methods: Postmortem blocks of olfactory mucosa were obtained from 12 AD cadavers and 24 controls and subjected to amyloid-beta and PHF-tau immunohistochemistry. Results: We observed marked heterogeneity in the presence of the biomarkers of tau and amyloid-beta among the targeted olfactory epithelial regions. No significant difference was observed between the cadavers with AD and the controls regarding the concentration of these proteins in any of these epithelial regions. Only one correlation significant was evident, namely, that between the tau protein densities of the middle and the upper turbinate (r = .58, P = .002). Conclusion: AD-related biomarker heterogeneity, which has not been previously demonstrated, makes comparisons across studies difficult and throws into question the usefulness of OE amyloid-beta and PHF-tau biopsies in detecting AD.
- Discrimination of non-melanoma skin cancer and keratosis from normal skin tissue in vivo and ex vivo by Raman spectroscopy(2019) LIMA, Ana Mara Ferreira; DANIEL, Camila Ribeiro; NAVARRO, Ricardo Scarparo; BODANESE, Benito; PASQUALUCCI, Carlos Augusto; PACHECO, Marcos Tadeu Tavares; ZANGARO, Renato Amaro; JR, Landulfo SilveiraThis study aimed the diagnosis of non-melanoma skin cancer (basal cell carcinoma - BCC, squamous cell carcinoma - SCC) and actinic keratosis (AK) and normal tissue (NO) by means of Raman spectra collected in vivo and ex vivo as well as determine, through the main Raman features, which biochemical element present in the skin is related to the spectral changes in the lesions compared to normal tissue. A total of 471 Raman spectra in vivo and 227 spectra ex vivo from NO, BCC, SCC and AK tissues were collected using a dispersive Raman spectrometer (830 nm excitation, 30 s exposure time, 200 mW laser' power) as well as spectra of basal skin constituents (proteins, lipids, nucleic acids). Student's t-test (p < 0.01) was applied to identify the Raman peaks with significant differences of lesion compared to normal skin and discriminant analysis based on Euclidian and Mahalanobis distances were applied to the intensities of the significant peaks. The results showed better discrimination between lesions and normal tissue through Mahalanobis distance both in vivo and ex vivo, with sensitivity, specificity and overall accuracy of 94.1%, 93.6% and 93.8%, respectively, for the in vivo spectra, while the discrimination showed sensitivity, specificity and overall accuracy of 100% for ex vivo tissues. The use of the intensities of the significant Raman peaks in the discrimination model showed results compared to other multivariate methods and may allow faster processing time, increasing the possibility of clinical use.
- Unrevealing the role of a frontotemporal dementia protein (TDP-43 protein) in bipolar disorder(2019) NASCIMENTO, C.; VILLELA, P. Nunes; KIM, H. Kyunghee; OLIVEIRA, K. De; LEITE, R. E. Paraizo; FERRETTI-REBUSTINI, R. E. D. L.; GRINBERG, L. T.; SUEMOTO, C. K.; PASQUALUCCI, C. A.; NITRINI, R.; JACOB-FILHO, W.; BRENTANI, H. P.; LAFER, B.
- Pleural anthracosis as an indicator of lifetime exposure to urban air pollution: An autopsy-based study in Sao Paulo(2019) TAKANO, Ana Paula Cremasco; JUSTO, Lisie Tocci; SANTOS, Nathalia Villa dos; MARQUEZINI, Monica Valeria; ANDRE, Paulo Afonso de; ROCHA, Francisco Marcelo Monteiro da; PASQUALUCCI, Carlos Augusto; BARROZO, Ligia Vizeu; SINGER, Julio M.; ANDRE, Carmen Diva Saldiva De; SALDIVA, Paulo Hilario Nascimento; VERAS, Mariana MateraMany studies have been conducted to evaluate the association between air pollution and adverse health effects using a wide variety of methods to assess exposure. However, the assessment of individual long-term exposure to ambient air pollution is a challenging task and has not been evaluated in a large autopsy study. Our goal was to investigate whether exposure to urban air pollution is associated to the degree of lung anthracosis, considering modifying factors such as personal habits, mobility patterns and occupational activities. We conducted a study in Sao Paulo, Brazil from February 2017 to June 2018, combining epidemiological, spatial analysis and autopsybased approaches. Information about residential address, socio-demographic details, occupation, smoking status, time of residence in the city and time spent commuting was collected via questionnaires applied to the next-ofkin. Images of the pleura surface from upper and lower lobes were used to quantify anthracosis in the lungs. We used multiple regression models to assess the association between the amount of carbon deposits in human lungs, measured by the fraction of pleural anthracosis (FA), and potential explanatory variables. We analyzed 413 cases and our data showed that for each additional hour spent in daily commuting, the ratio FA/(1-FA) is multiplied by 1.05 (95% confidence interval: [1.02; 1.08]). The estimated coefficient for daily hours spent in traffic was not considerably affected by the inclusion of socio-demographic variables and smoking habits. We estimate a tobacco equivalent dose of 5 cigarettes per day in a city where annual PM2.5 concentration oscillates around 25 mu g/m(3). Pleural anthracosis is a potential index of lifetime exposure to traffic-derived air pollution.
conferenceObject Increased levels of cortisol but not C-reactive protein in different brain regions in bipolar disorder: a post-mortem study(2019) NUNES, P. V.; NASCIMENTO, C.; SUEMOTO, C. K.; RODRIGUEZ, R. D.; LEITE, R. E. P.; FERRETTI-REBUSTINI, R. E. delucena; GRINBERG, L. T.; PASQUALUCCI, C. A.; NITRINI, R.; JACOB-FILHO, W.; BRENTANI, H. P.; LAFER, B.- Layer-specific reduced neuronal density in the orbitofrontal cortex of older adults with obsessive-compulsive disorder(2019) OLIVEIRA, Katia Cristina de; GRINBERG, Lea Tenenholz; HOEXTER, Marcelo Queiroz; BRENTANI, Helena; SUEMOTO, Claudia Kimie; NERY, Fabiano Goncalves; LIMA, Luzia Carreira; ALHO, Ana Tereza Di Lorenzo; FARFEL, Jose Marcelo; FERRETTI-REBUSTINI, Renata Eloah de Lucena; LEITE, Renata Elaine Paraizo; MORETTO, Ariane Cristine; SILVA, Alexandre Valotta da; LAFER, Beny; MIGUEL, Euripedes Constantino; NITRINI, Ricardo; JACOB-FILHO, Wilson; HEINSEN, Helmut; PASQUALUCCI, Carlos AugustoNeurobiological models have provided consistent evidence of the involvement of cortical-subcortical circuitry in obsessive-compulsive disorder (OCD). The orbitofrontal cortex (OFC), involved in motivation and emotional responses, is an important regulatory node within this circuitry. However, OFC abnormalities at the cellular level have so far not been studied. To address this question, we have recruited a total of seven senior individuals from the Sao Paulo Autopsy Services who were diagnosed with OCD after an extensive post-mortem clinical evaluation with their next of kin. Patients with cognitive impairment were excluded. The OCD cases were age- and sex-matched with 7 control cases and a total of 14 formalin-fixed, serially cut, and gallocyanin-stained hemispheres (7 subjects with OCD and 7 controls) were analyzed stereologically. We estimated laminar neuronal density, volume of the anteromedial (AM), medial orbitofrontal (MO), and anterolateral (AL) areas of the OFC. We found statistically significant layer- and region-specific lower neuron densities in our OCD cases that added to a deficit of 25% in AM and AL and to a deficit of 21% in MO, respectively. The volumes of the OFC areas were similar between the OCD and control groups. These results provide evidence of complex layer and region-specific neuronal deficits/loss in old OCD cases which could have a considerable impact on information processing within orbitofrontal regions and with afferent and efferent targets.
- Low brain-derived neurotrophic factor levels in post-mortem brains is associated with sub-syndromic neuropsychiatric symptoms(2019) NUNES, P. V.; NASCIMENTO, C. F.; KIM, H. K.; ANDREAZZA, A.; BRENTANI, H. P.; SUEMOTO, C. K.; LEITE, R. E. P.; FERRETTI-REBUSTINI, R. E. D. L.; PASQUALUCCI, C. A.; NITRINI, R.; GRINBERG, L. T.; YOUNG, L. T.; JACOB-FILHO, W.; LAFER, B.