CARLOS AUGUSTO GONCALVES PASQUALUCCI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
conferenceObject Inflammatory factors (cytokines and cortisol) across different brain regions in bipolar disorder and their associations with neuropsychiatric symptoms: A post-mortem study(2020) NASCIMENTO, Camila; NUNES, Paula V.; SUEMOTO, Claudia K.; RODRIGUEZ, Roberta D.; LEITE, Renata E. P.; GRINBERG, Lea T.; PASQUALUCCI, Carlos A.; NITRINI, Ricardo; JACOB-FILHO, Wilson; BRENTANI, Helena P.; LAFER, Beny- Evaluation of the diaphragm muscle remodeling, inflammation, oxidative stress and vascularization in smokers: An autopsy study(2020) NUCCI, R.A.B.; MAIFRINO, L.B.M.; BUSSE, A.L.; SOUZA, R.R. De; PASQUALUCCI, C.A.; ANARUMA, C.A.; LEITE, R.E.P.; RODRIGUEZ, R.D.; SUEMOTO, C.K.; JACOB-FILHO, W.Background/Aims: Cigarette smoking is a key factor in systemic inflammation and oxidative stress, and it has also been associated with the loss of muscle strength and an elevated risk of pulmonary diseases. Thus, this study aimed to analyze the effects of cigarette smoking on the diaphragm muscle structure of postmortem samples. Methods: Immunohistochemical techniques were used for muscle remodeling (metalloproteinases 2 and 9), inflammation (cyclooxygenase-2), oxidative stress (8-hydroxy-2'-deoxyguanosine), and vascularization (vascular endothelial growth factor). Hematoxylin and eosin stain was used for histopathological analysis and Picrosirius stain was used to highlight the collagen fibers. Results: Cigarette smokers had an increase of diaphragm muscle remodeling, oxidative stress, inflammation, and vascularization compared to non-smokers. Conclusion: Diaphragm muscle structure may be negatively affected by cigarette smoking. © 2020 The Author(s).
- Determinants of microcephaly in adults using data mining: increased risk for dementia and greater association with male gender(2020) MANCINE, L.; SUEMOTO, C. K.; RODRIGUEZ, R. D.; LEITE, R. E. P.; NASCIMENTO, C.; FERRETI-REBUSTINI, R.; TEIXEIRA, J. D. M.; PASQUALUCCI, C. A.; NITRINI, R.; JACOB-FILHO, W.; GRINBERG, L.; SALVINI, R.; NUNES, P. V.
- Normal-subtracted preprocessing of Raman spectra aiming to discriminate skin actinic keratosis and neoplasias from benign lesions and normal skin tissues(2020) JR, Landulfo Silveira; PASQUALUCCI, Carlos Augusto; BODANESE, Benito; PACHECO, Marcos Tadeu Tavares; ZANGARO, Renato AmaroThe differences in the biochemistry of normal and cancerous tissue could be better exploited by Raman spectroscopy when the spectral information from normal tissue is subtracted from the abnormal tissues. In this study, we evaluated the use of the normal-subtracted spectra to evidence the biochemical differences in the pre-cancerous and cancerous skin tissues compared with normal skin, and to discriminate the groups with altered tissues with respect to the normal sites. Raman spectra from skin tissues [normal (Normal), benign (dermatitis-BEN), basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and actinic keratosis (KER)] were obtained in vivo (Silveira et al., 2015, doi: 10.1002/lsm.22318) and used to develop the spectral model. The mean spectrum of the normal sites (circumjacent to each lesion) from each subject was calculated and subtracted from each individual spectrum of that particular subject independently of the group (Normal, BEN, BCC, SCC, KERAT). The mean spectra of each altered group and the mean spectra of the differences were firstly evaluated in terms of biochemical contribution or differentiation comparing the normal site. Then, the normal-subtracted spectra were submitted to discriminant models based on partial least squares and principal components regression (PLS-DA and PCR-DA), and the discrimination were compared with the model using non-subtracted spectra. Results showed that the peaks of nucleic acids, lipids (triolein) and proteins (elastin and collagens I, III, and IV) were significantly different in the lesions, higher for the pre- and neoplastic lesions compared with normal and benign. The PLS-DA showed that the groups could be discriminated with 90.3% accuracy when the mean-subtracted spectra were used, contrasting with 75.1% accuracy when the non-subtracted spectra were used. Also, when discriminating non-neoplastic tissue (Normal + BEN) from pre- and neoplastic sites (BCC + SCC + KERAT), the accuracy increases to 92.5% for the normal-subtracted compared with 85.3% for the non-subtracted. The subtraction of the mean normal spectrum from the subject obtained circumjacent to each lesion could significantly increase the diagnostic capability of the Raman-based discrimination algorithm.
conferenceObject Disrupted Genes Modules in the Hippocampus of Older Adults With Bipolar Disorder(2020) NASCIMENTO, Camila; BARBOSA, Andre; NUNES, Paula; SUEMOTO, Claudia; LEITE, Renata; GRINBERG, Lea; PASQUALUCCI, Carlos; NITRINI, Ricardo; JACOB-FILHO, Wilson; BRENTANI, Helena; LAFER, Beny- Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study(2020) NASCIMENTO, Camila; NUNES, Paula Villela; SUEMOTO, Claudia Kimie; RODRIGUEZ, Roberta Diehl; LEITE, Renata Elaine Paraizo; GRINBERG, Lea Tenenholz; PASQUALUCCI, Carlos Augusto; NITRINI, Ricardo; JACOB-FILHO, Wilson; BRENTANI, Helena Paula; LAFER, Beny
- Diaphragm muscle structure in the elderly: Findings from an autopsy study(2020) NUCCI, Ricardo Aparecido Baptista; SOUZA, Romeu Rodrigues de; SUEMOTO, Claudia Kimie; BUSSE, Alexandre Leopold; MAIFRINO, Laura Beatriz Mesiano; ANARUMA, Carlos Alberto; PASQUALUCCI, Carlos Augusto; JACOB-FILHO, WilsonSarcopenia is characterized as aging-related weakness and atrophy, which decreases the diaphragm force generating capacity leading to a decrease of expulsive non-ventilatory motor behaviors, which are critical for airway clearance. Thus, this study aimed to analyze the histopathology of the diaphragm in postmortem samples. Thirty individuals were included. Diaphragm samples were stained with hematoxylin and eosin for histopathological analysis. Picrosirius stain was used to highlight the collagen fibers. We observed a positive association between advancing age and histopathological findings in the diaphragm structure. We suggest that age is a key factor in increasing diaphragm muscle histopathology. However, further clinicopathological studies are needed to confirm our findings.
- Response letter: neuropathological lesions in the very old(2020) SUEMOTO, Claudia K.; LEITE, Renata E. P.; FERRETTI-REBUSTINI, Renata E. L.; RODRIGUEZ, Roberta D.; NITRINI, Ricardo; PASQUALUCCI, Carlos A.; JACOB-FILHO, Wilson; GRINBERG, Lea T.
conferenceObject Increased Levels of Inflammatory Cytokines across Different Brain Regions in Bipolar Disorder and its Correlation With Cortisol and Neuropsychiatric Symptoms: A Post-Mortem Study(2020) NUNES, Paula; NASCIMENTO, Camila; SUEMOTO, Claudia; RODRIGUEZ, Roberta; LEITE, Renata; GRINBERG, Lea; PASQUALUCCI, Carlos; NITRINI, Ricardo; JACOB-FILHO, Wilson; LAFER, Beny