SIMONE APARECIDA DE BESSA GARCIA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Gene expression profiling of triple-negative breast tumors with different expression of secreted protein acidic and cysteine rich (SPARC)(2018) ALCANTARA FILHO, Paulo R. de; MANGONE, Flavia R.; PAVANELLI, Ana C.; GARCIA, Simone A. de Bessa; NONOGAKI, Suely; OSORIO, Cynthia A. B. de Toledo; ANDRADE, Victor P. de; NAGAI, Maria A.Aim: To determine the expression signature of triple-negative breast cancer (TNBC) with differences of secreted protein acidic and rich in cysteine expression and clinical behavior. Patients, materials & methods: cDNA microarray analysis was performed to determine the expression profiling of TNBC, characterized regarding secreted protein acidic and rich in cysteine expression status. Immunohistochemistry analysis on tissue microarrays containing an independent cohort of TNBC was performed for validation. Results: Negative staining of SOHLH2 and positive staining of DNAJC12 and LIM1 was correlated with a poor outcome of the patients. Conclusion: Our findings provide new information on transcriptome changes associated with the clinical behavior of TNBC that may serve as a potential tool for the identification and characterization of new candidate biomarkers.
- Prostate apoptosis response-4 is involved in the apoptosis response to docetaxel in MCF-7 breast cancer cells(2013) PEREIRA, Michelly C.; BESSA-GARCIA, Simone A. De; BURIKHANOV, Ravshan; PAVANELLI, Ana Carolina; ANTUNES, Lourival; RANGNEKAR, Vivek M.; NAGAI, Maria A.Experimental evidence indicates that prostate apoptosis response-4 (Par-4, also known as PAWR) is a key regulator of cancer cell survival and may be a target for cancer-selective targeted therapeutics. Par-4 was first identified in prostate cancer cells undergoing apoptosis. Both intracellular and extracellular Par-4 have been implicated in apoptosis. Relatively little is known about the role of Par-4 in breast cancer cell apoptosis. In this study, we sought to investigate the effects of Par-4 expression on cell proliferation, apoptosis and drug sensitivity in breast cancer cells. MCF-7 cells were stably transfected with expression vectors for Par-4, or transiently transfected with siRNA for Par-4 knockdown. Cell proliferation assays were performed using MTT and apoptosis was evaluated using acridine orange staining, fluorescence microscopy and flow cytometry. Par-4 overexpression reduced MCF-7 proliferation rates. Conversely, Par-4 knockdown led to increased MCF-7 proliferation. Par-4 downregulation also led to increased BCL-2 and reduced BID expression. Par-4 overexpression did not affect the cell cycle profile. However, MCF-7 cells with increased Par-4 expression showed reduced ERK phosphorylation, suggesting that the inhibition of cell proliferation promoted by Par-4 may be mediated by the MAPK/ERK1/2 pathway. MCF-7 cells with increased Par-4 expression showed a marginal increase in early apoptotic cells. Importantly, we found that Par-4 expression modulates apoptosis in response to docetaxel in MCF7 breast cancer cells. Par-4 exerts growth inhibitory effects on breast cancer cells and chemosensitizes them to docetaxel.
- Transcriptome analysis of MCF7 breast cancer cells with different expression profile of PAR-4 (prostate apoptosis response-4) in the presence and absence of docetaxel(2015) GARCIA, Simone A. de Bessa; PAVANELLI, Ana C.; MELO, Natalia C.; NAGAI, Maria A.
conferenceObject Prostate apoptosis response-4 (PAR4) secretion in breast tumor and normal cell lines cultured in hypoxic conditions(2018) GARCIA, Simone Aparecida de Bessa; BROBOVNITCHAIA, Irina Gueroldovna; MANGONE, Flavia Rotea; NAGAI, Maria Aparecida