SANDRA NASSA SAMPIETRE

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Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina

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  • article 19 Citação(ões) na Scopus
    Trisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusion
    (2016) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; COELHO, Ana Maria Mendonca; SAMPIETRE, Sandra N.; PATZINA, Rosely Antunes; ABDO, Emilio Elias; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo Andrade; GODOY, Carlos M. G.; RODRIGUES, Tiago; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.
    Background Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives The aims of this research are to evaluate TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods Wistar rats submitted to partial liver ischemia were divided in groups: Control: (n = 10): surgical manipulation with no liver ischemia; Saline: (n = 15): rats receiving IV saline before reperfusion; and TD: (n = 15): rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-alpha, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and after treatment with TD. Results AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline Group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.
  • article 8 Citação(ões) na Scopus
    Ischemic Preconditioning-Like Effect of Polyunsaturated Fatty Acid-Rich Diet on Hepatic Ischemia/Reperfusion Injury
    (2011) COELHO, Ana Maria Mendonca; MACHADO, Marcel Cerqueira Cesar; TAKAHASHI, Hilton Kenji; SAMPIETRE, Sandra N.; STEFANO, Jose Tadeu; LEITE, Andre Zonetti A.; CURI, Rui; D'ALBUQUERQUE, Luiz A. Carneiro
    Aim The aim of this study was to investigate a possible preconditioning effect of oral diet enriched with polyunsaturated fatty acids (PUFAs) on liver ischemia/reperfusion (I/R) injuries. Methods Wistar male rats were fed a standard diet or polyunsaturated fatty acid-rich diet (PRD) enriched with (GII) or without (GIII) omega-3 PUFA. Rats were submitted to partial liver ischemia during 1 h and evaluated in pre- and post-I/R conditions. In pre-I/R condition, livers were collected for determination of fatty acid composition, liver mitochondrial function, malondialdehyde (MDA) content, and histological analysis. Four hours after liver reperfusion serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), serum levels of tumor necrosis factor-alpha, interleukin-6, interleukin-10, and prostaglandin-E2, liver mitochondrial function, MDA content, and histology were evaluated. Results In the pre-I/R condition, GII and GIII groups had an increase on PUFA content and exhibited slight increased macrosteatosis and microsteatosis in the liver. After 4 h of reperfusion, PRD-fed rats showed a marked decrease on steatosis, diminished necrosis, an increase in MDA formation, and mitochondrial uncoupling. We also observed a marked decrease in plasma levels of cytokines and ALT and AST activities in post-I/R condition in PRD groups. Conclusion In this experimental model in the rat, PRD has a preconditioning effect protecting the liver from I/R injury and should be object of future clinical studies.
  • conferenceObject
    Heparin Fragments Effects in Liver Injury Secondary to Liver Ischemia/Reperfusion (I/R)
    (2014) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; ABDO, Emilio E.; SAMPIETRE, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne S.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.
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    THE MRNA EXPRESSION OF SERCA AND NCX IN ACUTE PANCREATITIS MODEL UNDER MELATONIN AND TRISULFATE DISSACHARIDE ACTION
    (2017) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; KUBRUSLY, Marcia S.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; RODRIGUES, Tiago; NADER, Helena; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.
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    Effects of Intravenous Administration of Pentoxifylline in Pancreatic Ischemia/Reperfusion Injury
    (2012) CAMPION, Edmond R.; COELHO, Ana Maria M.; MACHADO, Marcel C.; SAMPIETRE, Sandra N.; MOLAN, Nilza A.; JUKEMURA, Jose; D'ALBUQUERQUE, Luiz C.
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    Sevofluorane Reduces Liver Damage Secondary to Ischemic/Reperfusion Injury by a Mechanism Not Related to a Preconditioning Effect
    (2012) CAVALCANTE, Fernanda P.; COELHO, Ana Maria M.; MACHADO, Marcel C.; SAMPIETRE, Sandra N.; MOLAN, Nilza A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz C.
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    LOCAL AND SYSTEMIC EFFECTS OF AGING ON ACUTE PANCREATITIS
    (2019) MACHADO, Marcel C. C.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; PINHEIRO, Fabiano; CUNHA, Jose Eduardo M.; D'ALBUQUERQUE, Luiz C.
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    Low Molecular Weight Heparin Fragment Decreases Intracellular Calcium in Human Hepatocarcinoma Cells Under Calcium Overload
    (2016) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; ABDO, Emilio E.; NADER, Helena B.; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; D'ALBUQUERQUE, Luiz Augusto C.; CHAIB, Eleazar; RODRIGUES, Tiago
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    Aging is Related to Increased Intestinal Damage and Bacterial Translocation in Acute Pancreatitis in Rats
    (2012) COELHO, Ana Maria M.; MACHADO, Marcel C.; SAMPIETRE, Sandra N.; MOLAN, Nilza A.; HEIJDEN, Inneke M. van der; CUNHA, Jose Eduardo M.; D'ALBUQUERQUE, Luiz C.
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    Anti-Inflammatory Effects of Hypertonic Saline Solution in Pancreatic Ischemia/Reperfusion Injuries
    (2012) GODOY, Renato S.; MARIA, Ana; COELHO, M.; SAMPIETRE, Sandra N.; MOLAN, Nilza A.; TAKAYANAGI, Oscar M.; MACHADO, Marcel C.; JUKEMURA, Jose; D'ALBUQUERQUE, Luiz C.