MARCELO ANDREETTA CORRAL
Projetos de Pesquisa
Unidades Organizacionais
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject POTENTIAL IMMUNOLOGICAL MARKERS FOR DIAGNOSIS OF HUMAN STRONGYLOIDIASIS USING HETEROLOGOUS ANTIGENS(2017) GRYSCHEK, Ronaldo; CORRAL, Marcelo; PAULA, Fabiana; MEISEL, Dirce; CASTILHO, Vera; GONCALVES, Elenice; LEVY, Debora; BYDLOWSKI, Sergio; CHIEFFI, Pedro Paulo; CASTRO-BORGES, WilliamconferenceObject DIFFERENTIAL EXPRESSION OF MEMBRANE AND MEMBRANE-BOUND PROTEINS FROM FILARIFORM LARVAE AND ADULT FEMALE OF STRONGYLOIDES VENEZUELENSIS(2017) PAULA, Fabiana M.; CORRAL, Marcelo A.; MARQUES, Priscilla D.; MEISEL, Dirce Mary C.; COSTA-CRUZ, Julia Maria; SANTO, Maria Cristina Espirito; CAMPOS, Jonatan M.; MATTEI, Bruno; BORGES, William Castro; GRYSCHEK, Ronaldo Cesar- Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens(2017) CORRAL, M. A.; PAULA, F. M.; MEISEL, D. M. C. L.; CASTILHO, V. L. P.; GONCALVES, E. M. N.; LEVY, D.; BYDLOWSKI, S. P.; CHIEFFI, P. P.; CASTRO-BORGES, W.; GRYSCHEK, R. C. B.Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris-HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30-40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.
- Could mucin 16 and colony-stimulating factor 2-receptor beta possible graft versus host disease biomarkers? Medical hypotheses(2017) SOUZA, Milena Monteiro de; PAULA, Fabiana Martins de; HSIEH, Ricardo; MACEDO, Maria Cristina Martins Almeida; CORRAL, Marcelo Andreetta; NUNES, Thais Borguezan; PAULA, Fernanda De; LOURENCO, Silvia VanessaGraft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects. Samples were collected prospectively between pre-transplant, thirty days, one hundred and, two hundred days after transplant. The proteomic profile was analyzed using SDS-PAGE and LCMS-ESI-IT-TOF mass spectrometry. The relevant personal data, past medical history were also recorded. The most relevant proteins found exclusively in GVHD patients were: CSF2RB, Protocadherin (Pcdh) Fat 2 precursor, protein capicua homolog isoform CIC-S, MUC16 and RGPD8_HUMAN RANBP2. This study aims to conduct an initial evaluation of the possible presence of such biomarkers in saliva from GVHD patients, and suggested a potential application of proteomics analysis as a alternative method to diagnose GVHD.