WALCY PAGANELLI ROSOLIA TEODORO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
16 resultados
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conferenceObject Cigarette smoke exposure leads to bone resorption, matrix remodeling and a worsening in bone mineralization(2016) TEODORO, W. Rosolia; BARHOSA, A. Povoa; LOURENCO, J. Dias; VELOSA, A. P. Pereira; MARTINS, J.; OLIVO, C. Rosa; JORGETTI, V.; LOPES, F. D. T. Q. S.- Early type I collagen deposition is associated with prognosis in biliary atresia(2016) LONGO-SANTOS, Luis Ricardo; TEODORO, Walcy Rosolia; MELLO, Evandro Sobroza de; VELOSA, Ana Paula Pereira; PARRA, Edwin Roger; CAPELOZZI, Vera Luiza; TANNURI, UenisBackground: Biliary atresia (BA) is a cholestatic liver disease of children that progresses to hepatic fibrosis. BA is the main indication of pediatric liver transplantation (LTx). Histopathological markers in liver biopsies could be useful for predicting progression to end-stage disease. Objective: To establish histopathological or immunohistochemical markers in liver biopsies of BA patients and correlate those markers with prognosis. Method: Histological analysis of biliary alterations and morphometric assessment of liver fibrosis were performed, in addition to indirect immunofluorescence assays (IF) for type I, III, IV and V collagens in initial and final liver biopsies of 36 patients with BA who underwent Kasai hepatoportoenterostomy (KPE) and LTx in the last 20 years at a single center. Results: Histopathologicalmarkers had no correlation with evolutive time until LTx. The perisinusoidal deposition of type III and V collagens was more prominent in the initial biopsies (p < 0.01), whereas deposition of type I and IV collagens indicated progression (p < 0.01). Patients with large amounts of perisinusoidal type I collagen in the initial biopsies had worse progression time curves until LTx (p = 0.04). Conclusion: Morphometric assessment of perisinusoidal deposition of type I collagen by IF in the initial biopsy can correlate with progression time to LTx in post-surgical BA.
conferenceObject Unusual distribution of Type V collagen isoforms determines the cutaneous fibrosis in scleroderma(2016) TEODORO, W. Rosolia; MORAIS, J.; VELOSA, A. P. Pereira; MARTIN, P.; CARRASCO, S.; CAMARGO, L.; GOLDENSTEIN-SCHAINBERG, C.; CAPELOZZI, V.conferenceObject Fibril and network-forming collagen may influence crosslinked density of skin in diabetic rats(2016) TEODORO, W. Rosolia; TOLEDO, V. Protocevich; CATANOZI, S.; VELOSA, A. P. Pereira; SANTOS-FILHO, A. dos; CAPELOZZI, V.- Aerobic Exercise Attenuated Bleomycin-Induced Lung Fibrosis in Th2-Dominant Mice(2016) ANDRADE-SOUSA, Adilson Santos; PEREIRA, Paulo Rogerio; MACKENZIE, BreAnne; OLIVEIRA-JUNIOR, Manoel Carneiro; ASSUMPCAO-NETO, Erasmo; BRANDAO-RANGEL, Maysa Alves Rodrigues; DAMACENO-RODRIGUES, Nilsa Regina; CALDINI, Elia Garcia; VELOSA, Ana Paula Pereira; TEODORO, Walcy Rosolia; OLIVEIRA, Ana Paula Ligeiro de; DOLHNIKOFF, Marisa; EICKELBERG, Oliver; VIEIRA, Rodolfo PaulaIntroduction The aim of this study was to investigate the effect of aerobic exercise (AE) in reducing bleomycin- induced fibrosis in mice of a Th2-dominant immune background (BALB/c). Methods BALB/c mice were distributed into: sedentary, control (CON), Exercise-only (EX), sedentary, bleomycin-treated (BLEO) and bleomycin-treated+exercised (BLEO+EX); (n = 8/group). Following treadmill adaptation, 15 days following a single, oro-tracheal administration of bleomycin (1.5U/kg), AE was performed 5 days/week, 60min/day for 4 weeks at moderate intensity (60% of maximum velocity reached during a physical test) and assessed for pulmonary inflammation and remodeling, and cytokine levels in bronchoalveolar lavage (BAL). Results At 45 days post injury, compared to BLEO, BLEO+EX demonstrated reduced collagen deposition in the airways (p<0.001) and also in the lung parenchyma (p<0.001). In BAL, a decreased number of total leukocytes (p<0.01), eosinophils (p<0.001), lymphocytes (p<0.01), macrophages (p<0.01), and neutrophils (p<0.01), as well as reduced pro-inflammatory cytokines (CXCL-1; p<0.01), (IL-1 beta; p<0.001), (IL-5; p<0.01), (IL-6; p<0.001), (IL-13; p<0.01) and pro-fibrotic growth factor IGF-1 (p<0.001) were observed. Anti-inflammatory cytokine IL-10 was increased (p<0.001). Conclusion AE attenuated bleomycin-induced collagen deposition, inflammation and cytokines accumulation in the lungs of mice with a predominately Th2-background suggesting that therapeutic AE (15-44 days post injury) attenuates the pro-inflammatory, Th2 immune response and fibrosis in the bleomycin model.
conferenceObject Pulmonary and cutaneous remodeling in mice model of systemic sclerosis(2016) TEODORO, W. Rosolia; SANTOS, L. Araujo Dos; CATANOZI, S.; ANDRADE, P. C.; SANTOS, A. Filho Dos; FERNEZLIAN, S. De Moraes; EHER, E. Miristene; VELOSA, A. P. Pereira; CAPELOZZI, V. F.conferenceObject Adventitial Remodeling Of Experimental Pulmonary Hypertension Induces Myofibroblast-Produced Collagen V In A Th17-Pathways-Related Manner(2016) FABRO, A. T.; MACHADO, J. R.; TEODORO, W. R.; SANTOS, A. L. Dos; SANTOS, A. A. Dos; CRUNIVEL, H. R.; MILSONI, P. F.; FERNEZLIAN, S. D. M.; SANTOS, A. B. G. Dos; OLIVEIRA, R. A.; MEDEIROS, M. C. R.; RAINHO, C. A.; CAPELOZZI, V. L.article 2 Citação(ões) na Scopus Radiofrequency preserves histoarchitecture and enhances collagen synthesis in experimental tendon injury(2016) AKAMATSU, Flavia Emi; SALEH, Samir Omar; HOJAIJ, Flavio; AUGUSTO, Carlos; MARTINEZ, Real; ANDRADE, Mauro; TEODORO, Walcy Rosolia; JACOMO, Alfredo LuizWe investigated the action of radiofrequency (RF) on the healing process after inducing experimental lesions of the Achilles tendon in rats. Wistar rats were surgically subjected to bilateral partial transverse sectioning of the Achilles tendon. The right tendon was treated with radiofrequency (RFT), whereas the left tendon served as a control (CT). On the third postoperative day, the rats were divided into three experimental groups consisting of ten rats each, which were treated with monopolar radiofrequency (Tonederm (TM)) until they were sacrificed on the 7th, 14th or 28th days. The histological specimens were studied for inflammatory cell content, collagen types I and III, immunostaining and morphometry. Total collagen were biochemically analyzed and to evalute fibroblast and myofibroblast proliferation by vimentin and a-actin smooth muscle immunohistochemistry methods. Statistical analysis was performed using the Student's t-test, the sign test and the Kruskal-Wallis test to compare tendons treated with radiofrequency with the non-treated tendons (alpha=5%; alpha=10%). Larger amounts of collagen I with hydroxyproline content and myofibroblast cells were clearly evident within 7 days (p<0.05). No difference was observed in the inflammatory cell content between the groups. We found better collagen arrangement with RF administration across the entire time studied. Radiofrequency administration preserves histoarchitecture and enhances collagen synthesis during the initial phases of cicatrization, suggesting that the treatment can provide improved stiffness during the most vulnerable phases of tendon healing. Clinical studies may include RF among the therapeutic tools in tendinous lesion management.conferenceObject LOW-SALT DIET INDUCES ATHEROSCLEROSIS INDEPENDENT OF LOWERING BLOOD PRESSURE IN HYPERTENSIVE MICE(2016) CATANOZI, S.; FUSCO, F.; GOMES, D.; BISPO, K.; TOLEDO, V.; BARBEIRO, D.; CAPELOZZI, V.; FURUKAWA, L.; VELOSA, A. P.; TEODORO, W.; HEIMANN, J.; QUINTAO, E.; PASSARELLI, M.; NAKANDAKARE, E.conferenceObject Adventitial remodeling of experimental pulmonary hypertension induces myofibroblast-produced collagen V in a Th17-pathways-related manner(2016) FABRO, A. T.; SANTOS, A. L. dos; LEAO, P. d. Santos; CRUNIVEL, H. R.; MACHADO, J. R.; OLIVEIRA, R. A.; RAINHO, C. A.; VELOSA, A. P. Pereira; TEODORO, W. R.; CAPELOZZI, V.