RONALDO CESAR BORGES GRYSCHEK
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina - Docente
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
22 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 22
conferenceObject DETECTION OF SCHISTOSOMA MANSONI SPOROCYST STAGE IN BIOMPHALARIA GLABRATA MOLLUSK IN EXPERIMENTAL CONDITIONS(2018) CASOTTI, Marcia; TUAN, Roseli; GOMES, Michele; LUNA, Expedito Albuquerque; DIAS-NETO, Emmanuel; PAULA, Fabiana; PINHO, Joao Rebello; CARRILHO, Flair; GRYSCHEK, Ronaldo Borges; ESPIRITO-SANTO, MariaconferenceObject MOLECULAR CHARACTERIZATION OF THE LARVAL PHASE OF SCHISTOSOMA MANSONI IN BIOMPHALARIA GLABRATA MOLLUSKS UNDER EXPERIMENTAL CONDITIONS(2017) CASOTTI, Marcia Oliveira; TUAN, Roseli Tuan; GOMES, Michele; DIAS-NETO, Emmanuel; PINHO, Joao Renato Rebello; PAULA, Fabiana Martins; CARRILHO, Flair Jose Carrilho Jose; LUNA, Expedito Jose Albuquerque; GRYSCHEK, Ronaldo Cesar Borges Borges; ESPIRITO-SANTO, Maria Cristina- TWO SEQUENTIAL PCR AMPLIFICATIONS FOR DETECTION OF Schistosoma mansoni IN STOOL SAMPLES WITH LOW PARASITE LOAD(2012) ESPIRITO-SANTO, Maria Cristina Carvalho do; ALVARADO-MORA, Monica Viviana; PINTO, Pedro Luiz Silva; CARRILHO, Flair Jose; PINHO, Joao Renato Rebello; GRYSCHEK, Ronaldo Cesar BorgesSchistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate/phenol/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load.
- Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostics(2020) FONSECA, Priscilla D. M.; CORRAL, Marcelo A.; COSENZA-CONTRERAS, Miguel; MEISEL, Dirce M. C. L.; MELO, Gessica B.; ANTUNES, Milena M. S.; SANTO, Maria C. E.; GRYSCHEK, Ronaldo C. B.; COSTA-CRUZ, Julia M.; CASTRO-BORGES, William; PAULA, Fabiana M.Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We believe that the protein profile presented in this shotgun analysis extends our understanding of the protein composition within the Strongyloides genus, opening up new perspectives for research on biomarkers that may help with the diagnosis of human strongyloidiasis. Data are available via ProteomeXchange with identifier PXD013703.
- Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, Sao Paulo, Brazil(2019) MELO, Gessica Baptista de; MALTA, Fernanda de Mello; MARUTA, Celina Wakisaka; CRIADO, Paulo Ricardo; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; ESPIRITO-SANTO, Maria Cristina de Carvalho do; PAULA, Fabiana Martins de; GRYSCHEK, Ronaldo Cesar BorgesBlastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity and epidemiology are still controversial. Currently, it has been associated with intestinal diseases such as irritable bowel syndrome and clinical manifestations of allergic skin, such as chronic urticaria. In the context of urticaria, it is still uncertain whether this organism is directly related to the allergic manifestation or just a common component of the intestinal microbiota. This study aimed to evaluate the occurrence and molecular diversity of Blastocystis sp. in individuals with urticaria from a dermatology outpatient clinic, Sao Paulo, Brazil. Fecal samples of 58 patients with urticaria were examined using parasitological methods; and subsequently tested by polymerase chain reaction using Blastocystis-specific primers. The subtypes (STs) and alleles (a) were determined using BLASTn and MLST tools. ST1, ST2, ST3, ST4, ST6 and mixed infection (ST1 + ST3) were identified in the patients with urticaria; ST1 (a4), ST3 (a34 and a36) and ST4 (a42) were the most prevalent. Our molecular analyses allowed an initial description of Blastocystis subtypes in patients with urticaria from Sao Paulo city, Brazil. (C) 2019 Published by Elsevier Ltd on behalf of World Federation of Parasitologists.
- Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostic (vol 235, 111249, 2020)(2020) FONSECA, Priscilla D. M.; CORRAL, Marcelo A.; COSENZA-CONTRERAS, Miguel; MEISEL, Dirce M. C. L.; MELO, Gessica B.; ANTUNES, Milena M. S.; SANTO, Maria C. E.; GRYSCHEK, Ronaldo C. B.; COSTA-CRUZ, Julia M.; CASTRO-BORGES, William; PAULA, Fabiana M.
conferenceObject REVALENCE OF SCHISTOSOMA MANSONI INFECTION AND OTHER PARASITIC DISEASES IN PERIPHERAL AREAS OF BARRA MANSA, RIO DE JANEIRO, BRAZIL(2017) ESPIRITO-SANTO, Maria Cristina C.; CHIEFFI, Pedro Paulo; PAULA, Fabiana Martins de; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; ORBAN, Magali; PINHO, Joao Renato Rebello; LUNA, Expedito Jose de Albuquerque; GRYSCHEK, Ronaldo Cesar BorgesconferenceObject GENETIC DIVERSITY OF BLASTOCYSTIS SUBTYPES IN PATIENTS WITH CHRONIC URTICARIA(2017) PAULA, Fabiana M.; MELO, Gessica B.; MALTA, Fernanda M.; MARUTA, Celina W.; CRIADO, Paulo R.; CASTILHO, Vera Lucia P.; GONCALVES, Elenice M. N.; SANTO, Maria Cristina Espirito; GRYSCHEK, Ronaldo CesarconferenceObject CLINICAL, LABORATORY AND EPIDEMIOLOGICAL PROFILE AND TREATMENT OF SEVERE SCHISTOSOMIASIS MANSONI IN A TEACHING HOSPITAL IN SAO PAULO CITY, BRAZIL(2015) MAGALHAES, Maira Reina; ESPIRITO-SANTO, Maria Cristina Carvalho; MORTARI, Naima; LUNA, Expedito Jose; GRYSCHEK, Ronaldo CesarconferenceObject DIFFERENTIAL EXPRESSION OF MEMBRANE AND MEMBRANE-BOUND PROTEINS FROM FILARIFORM LARVAE AND ADULT FEMALE OF STRONGYLOIDES VENEZUELENSIS(2017) PAULA, Fabiana M.; CORRAL, Marcelo A.; MARQUES, Priscilla D.; MEISEL, Dirce Mary C.; COSTA-CRUZ, Julia Maria; SANTO, Maria Cristina Espirito; CAMPOS, Jonatan M.; MATTEI, Bruno; BORGES, William Castro; GRYSCHEK, Ronaldo Cesar
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