RONALDO CESAR BORGES GRYSCHEK
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina - Docente
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
- Evaluation of the Dot-ELISA as a diagnostic test for human strongyloidiasis based on the detection of IgA in saliva(2020) BOSQUI, Larissa Rodrigues; CORRAL, Marcelo Andreetta; LEVY, Debora; BYDLOWSKI, Sergio Paulo; GRYSCHEK, Ronaldo Cesar Borges; CUSTODIO, Luiz Antonio; PAVANELLI, Wander Rogerio; CONCHON-COSTA, Ivete; COSTA-CRUZ, Julia Maria; PAULA, Fabiana Martins de; COSTA, Idessania NazarethThis study aimed to evaluate the use of saliva samples in the Dot-ELISA test for immunodiagnosis of human strongyloidiasis. The Dot-ELISA presented similar results to the ELISA test, with 70% and 60% sensitivity and 85% and 90% specificity, respectively, for IgA in the saliva. The Dot-ELISA with alternative saliva samples may be a suitable tool for diagnosing human strongyloidiasis, especially in populations with high levels of exposure to helminth.
- Diagnosis of human strongyloidiasis: Application in clinical practice(2021) COSTA, Idessania Nazareth; BOSQUI, Larissa Rodrigues; CORRAL, Marcelo Andreetta; COSTA-CRUZ, Julia Maria; GRYSCHEK, Ronaldo Cesar Borges; PAULA, Fabiana Martins deThis review considers the advantages and disadvantages of parasitological techniques, methods of detecting antibodies and antigens, as well as molecular biology techniques in the diagnosis of human strongyloidiasis. In addition, it elucidates the potential of different techniques for rapid and effective detection of clinical cases, thus enabling early treatment and preventing fatal consequences of this helminthiasis.
- IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients(2019) CORRAL, Marcelo Andreetta; PAULA, Fabiana Martins de; MEISEL, Dirce Mary C. L.; ABDALA, Edson; COSTA, Silvia Figueiredo; PIERROTTI, Ligia Camera; YAMASHIRO, Juliana; GONCALVES, Elenice M. do Nascimento; CASTILHO, Vera Lucia P.; CHIEFFI, Pedro Paulo; GRYSCHEK, Ronald Cesar B.Immunocompromised patients constitute a risk group for the development of severe clinical forms of human strongyloidiasis. The diagnosis of this infection is primarily performed by parasitological techniques, but with low sensitivity. Serological techniques appear as an alternative, especially with heterologous antigens use. The aim of this study was to perform the Western blot technique by using S. venezuelensis infective third stage larva (iL3) soluble (TS) and membrane (TM) saline antigens to reveal immunoreactive bands in immunocompromised patients with strongyloidiasis. Serum samples from 117 parasitologically well-characterized patients were divided into four groups: S. stercoralis positive and immunocompetent (S + IC); S. stercoralis positive and immunocompromised (S + IP); negative and immunocompetent (S-IC); negative and immunocompromised (S-IP). A 40-35 kDa band was recognized by 100% of patients in the S + IC group in both antigenic fractions, and by 62.5% and 50% in the S + IP group using the TS and TM fractions, respectively. A 29 kDa band was recognized by 86.3% and 72.7% (for TS and TM, respectively) of patients in the S + IC group, and only by 12.5% of patients in the S + IP group on the TM antigen. Regardless of the patients' immunological condition, the 40-35 kDa band from S. venezuelensis was detected more frequently and can be used as an important marker to the immunodiagnosis of human strongyloidiasis.