ROBERTA KARLA BARBOSA DE SALES
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Role of markers C-Reactive Protein, Procalcitonin and TREM-1 in diagnosis of infection pleural effusion(2018) ALVARENGA, Vanessa; ACENCIO, Milena; SALES, Roberta; SILVA, Aline; SILVA, Jucara; SILVA, Carlos; TEIXEIRA, Lisete Ribeiro; MARCHI, Evaldo
conferenceObject Overexpression of inhibitor of DNA-binding proteins and angiogenic markers have higher impact on survival of non small cell lung cancer patients(2012) SALES, Roberta; TUMA, Thayla; ACENCIO, Milena; TERRA, Ricardo; PARRA, Edwin; CAPELOZZI, Vera; ANTONANGELO, LeilaconferenceObject Do pre-analytical or analytical factors influence the performance of real time PCR tests in the diagnosis of tuberculosis?(2013) ANTONANGELO, Leila; CARNEVALE, Gabriela; MARCAL, Lia; SALES, Roberta; ACENCIO, Milena; VARGAS, FranciscoconferenceObject Influence of pre analytical and analytical variables in extraction, amplification and detection of M. tuberculosis in pleural fluid(2012) CARNEVALE, Gabriela; SALES, Roberta; VARGAS, Francisco; ACENCIO, Milena; MARCHI, Evaldo; ANTONANGELO, Leila- Treatment with Anti-VEGF and anti-EGFR in a model of malignant pleural effusion: Genic evaluation(2018) TEIXEIRA, Lisete Ribeiro; ALVARENGA, Vanessa; SALES, Roberta; SILVA, Carlos; ALMEIDA, Nicole; SOUSA, Lucas; SANTOS, Ana; CAPELOZZI, Vera; MARCHI, Evaldo; ACENCIO, Milena
- Efficacy of two fluorescence in situ hybridization (FISH) probes for diagnosing malignant pleural effusions(2013) ROSOLEN, Debora C. B.; KULIKOWSKI, Leslie D.; BOTTURA, Giorgio; NASCIMENTO, Amon M.; ACENCIO, Milena; TEIXEIRA, Lisete; VARGAS, Francisco S.; SALES, Roberta K.; ANTONANGELO, LeilaIt is difficult to differentiate tumor cells in pleural fluid from reactive benign mesothelium. Fluorescence in situ hybridization (FISH) can increase diagnostic accuracy. Two hundred pleural fluid samples were analyzed by using FISH probes for chromosomes 11 and 17. Histological analysis was used to diagnose cancer. Clinical, radiological, and histological data were used to exclude malignancy. Eighty-two pleural effusion samples had positive cytology, 51 were benign, and 67 were atypical, but inconclusive. The 82 positive cases were confirmed to be malignant. Among the 51 negative cytology cases, videothoracoscopy-guided pleural biopsy revealed malignancy in three; aneuploid cells were detected by FISH in all cases. In 43 of the 67 cases with inconclusive cytology, malignancy was confirmed based on histology and fluorescence in situ hybridization. One case of parapneumonic effusion with no evidence of cancer during clinical follow-up had a suspicious cytology and positive fluorescence in situ hybridization result. The remaining 23 cases had no histological, radiological, clinical, or genetic evidence of malignancy. This study demonstrated that cytogenetic analysis of fresh pleural fluid samples using only two FISH probes is a valuable ancillary method for the identification of malignant pleural effusion, particularly in cases in which oncotic cytology is inconclusive.