ROBERTA KARLA BARBOSA DE SALES
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
conferenceObject Brazilian Analysis of Lent Score: Predicting the Survival Rate of Malignant Pleural Effusion(2023) COLARES, P. F.; RIVAS, J. Delgado; BELON, C. E. F.; ACENCIO, M. M. P.; SALES, R. K.; TEIXEIRA, L. R.conferenceObject Production Of Inflammatory Mediators And Induction Of Apoptosis In Pleural Mesothelial Cells, Lung And Breast Cancer Cells Exposed To Talc Particles(2016) ACENCIO, M. M. P.; TEIXEIRA, L. R.; ALVARENGA, V. A.; SILVA, C. S. R.; SALES, R. K. B.; MARCHI, E.conferenceObject Tuberculous Empyema: Combined Intrapleural Therapy Might Be an Alternative(2023) COLARES, P. F.; RIVAS, J. Delgado; SCIORTINO, A. D.; SAAVEDRA, N. Medina; SALES, R.; TEIXEIRA, L. R.conferenceObject Minute Pulmonary Meningothelial-like Nodules as an Incidental Finding in Lung Biopsy: Case Report(2023) COLARES, P. F.; RIVAS, J. Delgado; SCIORTINO, A. d.; SALES, R.; TEIXEIRA, L. R.- Pleural fluid tumour markers in malignant pleural effusion with inconclusive cytologic results(2015) ANTONANGELO, L.; SALES, R. K.; CORA, A. P.; ACENCIO, M. M. P.; TEIXEIRA, L. R.; VARGAS, F. S.Background The presence of tumour cells in pleural fluid or tissue defines an effusion as malignant. Cytology analysis of the pleural fluid has about 60% diagnostic sensitivity. Several tests have been proposed to improve diagnosis-among them, the concentrations of tumour markers in pleural fluid. We evaluated whether the concentrations of tumour markers in pleural fluid could improve the diagnosis of malignant pleural effusion (MPE) when cytology is doubtful. Methods Lymphocytic pleural fluids secondary to tuberculosis or malignancy from 156 outpatients were submitted for cytology and tumour marker quantification [carcinoembryonic antigen (CEA), cancer antigen 15-3 (CA15-3), carbohydrate antigen 19-9 (CA19-9), cancer antigen 72-4 (CA72-4), cancer antigen 125 (CA125), and CYFRA 21-1). One-way analysis of variance, the Student t-test or Mann-Whitney test, and receiver operating characteristic curves were used in the statistical analysis. Results Concentrations of the tumour markers CEA, CA15-3, CA125, and CYFRA 21-1 were higher in MPES than they were in the benign effusions (p < 0.001), regardless of cytology results. The markers CA19-9 and CA72-4 did not discriminate malignant from benign effusions. When comparing the concentrations of tumour markers in MPES having positive, suspicious, or negative cytology with concentrations in benign effusions, we observed higher levels of CEA, CA15-3, CYFRA 21-1, and CA125 in malignant effusions with positive cytology (p = 0.003, p = 0.001, p = 0.002, and p = 0.001 respectively). In pleural fluid, only CA125 was higher in MPES with suspicious or negative cytology (p = 0.001) than in benign effusions. Conclusions Given high specificity and a sensitivity of about 60%, the concentrations of tumour markers in pleural effusions could be evaluated in cases of inconclusive cytology in patients with a high pre-test chance of malignancy or a history of cancer.
conferenceObject Pleural Effusion as an Unusual Manifestation in Melanoma. Pleural Metastasis(2021) RIVAS, J. Delgado; BRIDI, G. D.; ALMEIDA, G. C.; NASCIMIENTO, E.; COLARES, P. F.; SALES, R.; TEIXEIRA, L. R.- Effect of temperature and storage time on cellular analysis of fresh pleural fluid samples(2012) ANTONANGELO, L.; VARGAS, F. S.; ACENCIO, M. M. P.; CORA, A. P.; TEIXEIRA, L. R.; GENOFRE, E. H.; SALES, R. K. B.L. Antonangelo, F. S. Vargas, M. M. P. Acencio, A. P. Cora, L. R. Teixeira, E. H. Genofre and R. K. B. Sales Effect of temperature and storage time on cellular analysis of fresh pleural fluid samples Objective: Despite the methodological variability in preparation techniques for pleural fluid cytology, it is fundamental that the cells should be preserved, permitting adequate morphological classification. We evaluated numerical and morphological changes in pleural fluid specimens processed after storage at room temperature or under refrigeration. Methods: Aliquots of pleural fluid from 30 patients, collected in ethylenediaminetetraacetic acid-coated tubes and maintained at room temperature (21 degrees C) or refrigeration (4 degrees C) were evaluated after 2 and 6 hours and 1, 2, 3, 4, 7 and 14 days. Evaluation of cytomorphology and global and percentage counts of leucocytes, macrophages and mesothelial cells were included. Results: The samples had quantitative cellular variations from day 3 or 4 onwards, depending on the storage conditions. Morphological alterations occurred earlier in samples maintained at room temperature (day 2) than in those under refrigeration (day 4). Conclusions: This study confirms that storage time and temperature are potential pre-analytical causes of error in pleural fluid cytology.