HIRO GOTO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Medicina Preventiva, Faculdade de Medicina - Docente
LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina - Líder
10 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 10
- Recombinant Leishmania infantum Heat Shock Protein 83 for the Serodiagnosis of Cutaneous, Mucosal, and Visceral Leishmaniases(2014) CELESTE, Beatriz Julieta; SANCHEZ, Maria Carmen Arroyo; RAMOS-SANCHEZ, Eduardo Milton; CASTRO, Luiz Guilherme M.; COSTA, Francisco Assis Lima; GOTO, HiroRoutine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases.
conferenceObject Analysis of global gene expression of Leishmania infantum under Insulin-like growth factor I stimulus(2015) RAMOS-SANCHEZ, E. M.; NERLAND, A. H.; GOTO, H.conferenceObject Insulin-like growth factor-I expression in the pathogenesis of pancytopaenia in canine and hamster visceral leishmaniasis(2015) PINHO, F. A.; TORRES, A. R. A.; CARVALHO-DANTAS, I. M.; MIYASHIRO, S. I.; HAGIWARA, M. K.; GOTO, H.conferenceObject Evaluation of immunochromatographic assay with recombinant antigen K39, using whole blood, serum and oral fluid, in the diagnosis of visceral leishmaniasis in Brazilian endemic areas(2015) CELESTE, B. J.; SANCHEZ, M. C. A.; ALMEIDA, R. P. D.; BRANCO, C. M. F. Castelo; LINDOSO, J. A. L.; PANIAGO, A. M. M.; QUEIROZ, I. T.; FUJIMORI, M.; CARAMELO, L. D. C.; GOTO, H.conferenceObject ANTI-LEISHMANIA ANTIBODIES IN SAMPLES OF BLOOD DONORS FROM ENDEMIC AREAS OF BRAZIL(2017) FUJIMORI, Mahyumi; CARAMELO, Luiza da Cruz; DORNELAS, Marina Portanova; CELESTE, Beatriz Julieta; WENDEL, Silvano; GOTO, Hiro; SANCHEZ, Maria CarmenconferenceObject EFFECTS OF INSULIN-LIKE GROWTH FACTOR-I AND IL-4 ON LEISHMANIA (L.) INFANTUM-INFECTED HUMAN MACROPHAGES(2018) GOTO, Hiro; SEVILLANO, Orlando; REIS, Luiza; RAMOS-SANCHEZ, Eduardo- Association between Insulin-Like Growth Factor-I Levels and the Disease Progression and Anemia in Visceral Leishmaniasis(2019) PINHO, Flaviane Alves de; VENDRAME, Celia Maria Vieira; MACIEL, Bruna Leal Lima; SILVA, Lucilene dos Santos; MIYASHIRO, Samantha Ive; JERONIMO, Selma Maria Bezerra; GOTO, HiroWe analyzed the association between insulin-like growth factor-I (IGF-I) and the pathogenesis of anemia during active visceral leishmaniasis (VL). Serum levels of IGF-I, IGF-binding protein 3 (IGFBP3), and cytokines were measured in samples from individuals with active VL and cured VL, asymptomatic Leishmania-infected, and noninfected individuals. Then, we extended our analysis to VL dogs to evaluate hematimetric parameters, bone marrow alterations, and cytokine and IGF-I expression. We identified a positive correlation between lower IGF-I and IGFBP3 levels in active VL patients and lower hemoglobin levels. In infected dogs, there was a positive correlation between lower IGF-I expression in the bone marrow and lower peripheral blood hematocrit and hemoglobin levels. There was no correlation between decreased IGF-I level/expression and any measured cytokine serum levels in either host. The data suggest that low IGF-I expression is associated with pathogenesis of anemia in active VL, primarily in severe cases, by mechanisms other than alterations in cytokine production.
conferenceObject Anti-Leishmania antibodies in samples of blood donors from endemic areas of Brazil using ELISA with recombinant protein K39 of L. infantum(2015) SANCHEZ, M. C. A.; FUJIMORI, M.; CARAMELO, L. D. C.; DORNELAS, M. P.; WENDEL, S.; GOTO, H.conferenceObject Th2-skewed response depends on Insulin-like growth factor I in Leishmania (Leishmania) major-infection(2015) REIS, L. C.; RAMOS-SANCHEZ, E. M.; GOTO, H.conferenceObject INSULIN-LIKE GROWTH FACTOR-I AS EFFECTOR ELEMENT OF IL-4 EFFECT LEADING TO SUSCEPTIBILITY TO LEISHMANIA MAJOR INFECTION(2018) GOTO, Hiro; REIS, Luiza; RAMOS-SANCHEZ, Eduardo; PETITTO-ASSIS, Fabricio; NERLAND, Audun; HERNANDEZ-VALLADARES, Maria; SELHEIM, Frode; FLOETER-WINTER, Lucile