MARIANA MATERA VERAS
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
14 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 14
- Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats(2017) TURCO, Marcelo Pitelli; SOUZA, Alex Balduino de; SOUSA, Isida de Campos; FRATINI, Paula; VERAS, Mariana Matera; RODRIGUES, Marcio Nogueira; BESSA JR., Jose de; BROLIO, Marina Pandolphi; LEITE, Katia Ramos Moreira; BRUSCHINI, Homero; SROUGI, Miguel; MIGLINO, Maria Angelica; GOMES, Cristiano MendesAimsInvestigate the effect of a novel cell-based therapy with skeletal muscle-derived mononuclear cells (SMDMCs) in a rat model of stress urinary incontinence. MethodsMale Wistar-Kyoto rats' hind limb muscles were enzymatically dissociated, and SMDMCs were isolated without needing expansion. The cell population was characterized. Twenty female rats underwent urethrolysis. One week later, 10 rats received periurethral injection of 10(6) cells (SMDMC group), and 10 rats received saline injections (Saline group). Ten rats underwent sham surgery (Sham group). Four weeks after injection, animals were euthanized and the urethra was removed. The incorporation of SMDMCs in the female urethra was evaluated with fluorescence in situ hybridization for the detection of Y-chromosomes. Hematoxylin and eosin, Masson's trichrome staining, and immunohistochemistry for actin and myosin were performed. The muscle/connective tissue, actin and myosin ratios were calculated. Morphological evaluation of the urethral diameters and fractional areas of the lumen, mucosa, and muscular layer was performed. ResultsSMDMCs population was consistent with the presence of muscle cells, muscle satellite cells, perivascular cells, muscle progenitor cells, and endothelial cells. SMDMCs were incorporated into the urethra. A significant decrease in the muscle/connective tissue ratio was observed in the Saline group compared with the SMDMC and Sham groups. The proportions of actin and myosin were significantly decreased in the Saline group. No differences were observed in the morphometric parameters. ConclusionsSDMSC were incorporated into the rat urethra and promoted histological recovery of the damaged urethral sphincter, resulting in decreased connective tissue deposition and increased muscle content.
- PLURIPARIDADE NAS ÉGUAS: RELAÇÃO COM CARACTERÍSTICAS MATERNAS, PLACENTÁRIAS E NEONATAIS(2017) SERRANO-RECALDE, Elena Carolina; QUEIROZ-SILVA, Juliana Cristina; GUIMARÃES, Carina de Fatima; VERAS, Mariana Matera; BARBOSA, Roberta Galvano; ALONSO, Maria Augusta; FERNANDES, Claudia BarbosaAbstract The placenta connects the mare and the foal and it is one of the main organs responsible for fetal development and newborn’s morphometric characteristics. This function becomes even more important because a number of studies indicates that foals physical characteristics are able to predict their development in adult life. Therefore, the aim of this study was to correlate mares parity (1-5) and maternal characteristics with placental characteristics as well as foals size. Mares were categorized according to parity in G1: 1 parturation (n=4); G2: 2 parturations (n=6); G3: 3 parturations (n=7); G4: 4 parturations (n=5); and G5: 5 parturations (n=3). Results indicate that the mares pre- and post-parturition thoracic perimeter and weight had a positive correlation on foals weight (p=0.004/ R= 0.51; p=0.002/ R= 0.55; p=0.01/ R= 0.43), height (p=0.0005/ R= 0.60; p=0.001/ R= 0.57; p=0.005/ R= 0,50), and thoracic perimeter (p=0.0001/ R= 0.65; p≤0.0001/ R= 0.71; p=0.0002/ R= 0.64) at birth. Mares that were heavier at pre-partum had greater placental weight (p=0.01/R= 0.45) and delivered heavier foals (p=0.003/ R= 0.52) with greater thoracic perimeter (p=0.01/ R= 0.45). Foals of mares in G4 were heavier, probably indicating that the greater uterine size in multiparous mares allows greater placental coverage, greater fetomaternal surface contact and probably greater supply of nutrient to the fetus.
- Transcriptomic analysis of purified human cortical microglia reveals age-associated changes(2017) GALATRO, Thais F.; HOLTMAN, Inge R.; LERARIO, Antonio M.; VAINCHTEIN, Ilia D.; BROUWER, Nieske; SOLA, Paula R.; VERAS, Mariana M.; PEREIRA, Tulio F.; LEITE, Renata E. P.; MOLLER, Thomas; WES, Paul D.; SOGAYAR, Mari C.; LAMAN, Jon D.; DUNNEN, Wilfred den; PASQUALUCCI, Carlos A.; OBA-SHINJO, Sueli M.; BODDEKE, Erik W. G. M.; MARIE, Suely K. N.; EGGEN, Bart J. L.Microglia are essential for CNS homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. Overall, genes expressed by human microglia were similar to those in mouse, including established microglial genes CX3CR1, P2RY12 and ITGAM (CD11B). However, a number of immune genes, not identified as part of the mouse microglial signature, were abundantly expressed in human microglia, including TLR, F-c gamma and SIGLEC receptors, as well as TAL1 and IFI16, regulators of proliferation and cell cycle. Age-associated changes in human microglia were enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression and actin (dis)assembly. Limited overlap was observed in microglial genes regulated during aging between mice and humans, indicating that human and mouse microglia age differently.
- Early and late pulmonary effects of nebulized LPS in mice: An acute lung injury model(2017) COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; ALEMANY, Adair Aparecida dos Santos; BELOTTI, Luciano; ZATI, Douglas Hidalgo; CAVALCANTE, Marcela Frota; VERAS, Mariana Matera; RIBEIRO, Susan; KALLAS, Esper Georges; SALDIVA, Paulo Hilario Nascimento; DOLHNIKOTT, Marisa; SILVA, Luiz Fernando Ferraz daBackground and objective Acute respiratory distress syndrome (ARDS) has a high mortality rate of 35 - 46% depending on its severity. Animal models are crucial to better understand the pathophysiology of diseases, including ARDS. This study presents a feasible animal model of acute lung injury (ALI) using nebulized lipopolysaccharide (LPS) in a non-invasive approach, focusing on its short and long-term effects. Methods Mice received nebulized LPS or vehicle only (control group). Blood, BALF and lung tissue were collected 24 hours (LPS 24h) or 5 weeks (LPS 5w) after the nebulized LPS-induced lung injury. Inflammatory cytokines were assessed in the blood serum, BALF and lung tissue. Stereological analyses and remodeling changes were assessed by histology and immunohistochemistry at the specified time points. Results The LPS 24h group showed increased pro-inflammatory cytokine levels, intense cell influx, increased total septal volume, septal thickening and decreased surface density of the alveolar septa. The LPS 5w group showed persistent lung inflammation, septal thickening, increased total lung volume, accentuated collagen deposition, especially of collagen type I, and decreased MMP-2 protein expression. Conclusion We present a feasible, reproducible and non-invasive nebulized-LPS animal model that allows the assessment of both the acute and late phases of acute lung injury. The presence of lung remodeling with collagen deposition after 5 weeks makes it useful to study the pathophysiology, complications, and possible therapeutic intervention studies that aim to understand and reduce pulmonary fibrosis in the late phases of ALI.
conferenceObject Lasting effects of prenatal marijuana exposure on the retina: an experimental study in mice(2017) ZANTUT, Paulo; TAKAHASHI, Walter; YARIWAKE, Victor; TORRES, Janaina; PECORA, Ricardo; SAFATLE, Angelica; VERAS, Mariana; DAMICO, Francisco- Exposure to fine particulate matter in the air alters placental structure and the renin-angiotensin system(2017) SOTO, Sonia de Fatima; MELO, Juliana Oliveira de; MARCHESI, Guilherme D'Aprile; LOPES, Karen Lucasechi; VERAS, Mariana Matera; OLIVEIRA, Ivone Braga de; SOUZA, Regiane Machado de; CASTRO, Isac de; FURUKAWA, Luzia Naoko Shinohara; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel C.Transforming growth factor beta 1 (TGF beta 1), the uteroplacental renin-angiotensin system (RAS) and vascular endothelial growth factor A (VEGF-A) participate in the placentation process. The aim of this study was to investigate the effects of exposure to pollutants on the placenta. Methods Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning on day 6 of pregnancy. At embryonic day 19, the placenta was collected. The placental structure, the protein and gene expression of TGF beta 1, VEGF-A, and its receptor Flk-1 and RAS were evaluated by indirect ELISA and quantitative real-time PCR. Results Exposure to P decreased the placental mass, size, and surface area as well as the TGF beta 1, VEGF-A and Flk-1 content. In the maternal portion of the placenta, angiotensin II (AngII) and its receptors AT(1) (AT(1)R) and AT(2) (AT(2)R) were decreased in the PF and PP groups. In the fetal portion of the placenta, AngII in the FP, PF and PP groups and AT(2)R in the PF and PP groups were decreased, but AT(1)R was increased in the FP group. VEGF-A gene expression was lower in the PP group than in the FF group. Conclusions Exposure to pollutants before and/or during pregnancy alters some characteristics of the placenta, indicating a possible impairment of trophoblast invasion and placental angiogenesis with possible consequences for the maternal-fetal interaction, such as a limitation of fetal nutrition and growth.
- ADVANCED MATERNAL AGE AND PLACENTAL MORPHOFUNCTIONAL CHANGES: AN EXPERIMENTAL STUDY IN MICE(2017) TORRES, J. I.; NEVES, I. A.; YARIWAKE, V. Y.; MIGLINO, M. A.; VERAS, M. M.
- Ambient levels of concentrated PM2.5 affects cell kinetics in adrenal glands: an experimental study in mice(2017) FUCHS, Luiz Fernando Portugal; VERAS, Mariana Matera; SALDIVA, Paulo Hilario Nascimento; SASSO, Gisela Rodrigues da Silva; CARVALHO, Katia Candido; SIMOES, Manuel de Jesus; SOARES JR., Jose Maria; BARACAT, Edmund ChadaWe evaluated the effects of air pollution on the adrenal cortex using 30 female mice divided into two groups of fifteen animals each. One group was conditioned daily in a chamber with exposure to particulate matter (PM) 2.5 mu m (GExp). Animals were exposed on daily basis in an ambient particles concentrator during the period of time enough to reach an accumulated dose of 600 mu g/m(3), which corresponds to a 24-h exposure of 25 mu g/m(3) that approximates to the annual mean of PM2.5 in Sao Paulo. The other group was allocated to another chamber with filtered air (GCrt). After euthanasia, the adrenals underwent histological processing and immunohistochemistry staining for Ki-67 and cleaved caspase-3. Histomorphometry of the adrenal glands in GExp showed increased thickness of the zona glomerulosa, while in GCrt; the adrenal glands from GExp had higher Ki-67 immunostaining scores in the zona reticularis than those from GCrt. The adrenal from GExp showed higher cleaved caspase-3 immunoreactivity in the zona fasciculata than the unexposed group (GCrt). The homeostasis index indicated higher cell proliferation in the zona glomerulosa and zona reticularis in GExp than in GCrt. Our data indicate that PM2.5 air pollution induces alterations on cell kinetics in mouse adrenal glands.
- How Environmental and Air Pollution Disrupt Spermatogenesis and Male Reproductive Health(2017) HALLAK, J.; VERAS, M. M.; SALDIVA, P. H. N.Man is a masterpiece and is intimately linked to the environment from an evolutionary standpoint. The equation phenotype=genotype+environment that many of us learned at high school is just as relevant today, particularly in industrialized and polluted areas around the globe. © 2018 Elsevier Inc. All rights reserved.
- Influence of Maternal Age and Parity on Placental Structure and Foal Characteristics From Birth up to 2 Years of Age(2017) MEIRELLES, Marcela Goncalves; VERAS, Mariana Matera; ALONSO, Maria Augusta; GUIMARAES, Carina de Fatima; NICHI, Marcilio; FERNANDES, Claudia BarbosaThe foal's weight and height at birth are regarded as important parameters for the equestrian sports industry. Moreover, scientific studies indicate that maternal environment can influence postnatal genetic potential of foals. The purpose of this study was to evaluate the influence of maternal age and parity on placental efficiency and size of the newborn foal using stereological analysis tools of the term's allantochorion. Furthermore, the influence of maternal age and parity on weight, height, and girth circumference of the offspring from birth until 2 years of age was investigated. Forty pregnant mares were categorized according to age: 4-8 years (n = 15); 9-12 years (n = 15); >= 13 years (n = 10) and parity: nulliparous (n = 12), 1-3 parities (n = 18); >= 4 parities (n = 10). Parturition was monitored and the allantochorion membranes were collected after delivery. The stereological analysis determined the volumetric composition of the different compartments of the placenta along with the area of fetomaternal contact surface. It was observed that maternal age and parity significantly increased the development of the microcotyledons and total villi surface density, as well as the vascularization and tissue composition of different regions of the placenta, which contributes to fetomaternal contact. We also found a significant relationship between maternal age and parity on the size of the neonate foals but not after the first year of age, possibly because environmental and genetic factors contribute to postnatal growth.