ELIA TAMASO ESPIN GARCIA CALZOLARI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
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- Baroreflex Sensitivity Impairment Is Associated With Cardiac Diastolic Dysfunction in Rats(2011) MOSTARDA, Cristiano; MORAES-SILVA, Ivana Cinthya; MOREIRA, Edson Dias; MEDEIROS, Alessandra; PIRATELLO, Aline Cristina; CONSOLIM-COLOMBO, Fernanda Marciano; CALDINI, Elia Garcia; BRUM, Patricia Chakur; KRIEGER, Eduardo Moacyr; IRIGOYEN, Maria ClaudiaBackground: Studies have shown that the autonomic dysfunction accompanied by impaired baroreflex sensitivity was associated with higher mortality. However, the influence of decreased baroreflex sensitivity on cardiac function, especially in diastolic function, is not well understood. This study evaluated the morpho-functional changes associated with baroreflex impairment induced by chronic sinoaortic denervation (SAD). Methods and Results: Animals were divided into sinoaortic denervation (SAD) and control (C) groups. Baroreflex sensitivity was evaluated by tachycardic and bradycardic responses, induced by vasoactive drugs. Cardiac function was studied by echocardiography and by left ventricle (LV) catheterization. LV collagen content and the expression of regulatory proteins involved in intracellular Ca(2+) homeostasis were quantified. Results showed higher LV mass in SAD versus C animals. Furthermore, an increase in deceleration time of E-wave in the SAD versus the C group (2.14 +/- 0.07 ms vs 1.78 +/- 0.03 ms) was observed. LV end-diastolic pressure was increased and the minimum dP/dt was decreased in the SAD versus the C group (12 +/- 1.5 mm Hg vs 5.3 +/- 0.2 mm Hg and 7,422 +/- 201 vs 4,999 +/- 345 mm Hg/s, respectively). SERCA/NCX ratio was lower in SAD than in control rats. The same was verified in SERCA/PLB ratio. Conclusions: The results suggest that baroreflex dysfunction is associated with cardiac diastolic dysfunction independently of the presence of other risk factors. (J Cardiac Fail 2011;17:519-525)
- Baroreflex Impairment Precedes Cardiometabolic Dysfunction in an Experimental Model of Metabolic Syndrome: Role of Inflammation and Oxidative Stress(2018) BERNARDES, Nathalia; DIAS, Danielle da Silva; STOYELL-CONTI, Filipe Fernandes; BRITO-MONZANI, Janaina de Oliveira; MALFITANO, Christiane; CALDINI, Elia Garcia; ULLOA, Luis; LLESUY, Susana Francisca; IRIGOYEN, Maria-Claudia; ANGELIS, Katia DeThis study analyzes whether autonomic dysfunction precedes cardiometabolic alterations in spontaneously hypertensive rats (SHR) with fructose overload. Animals were randomly distributed into three groups: control, hypertensive and hypertensive with fructose overload. Fructose overload (100 g/L) was initiated at 30 days old, and the animals (n = 6/group/time) were evaluated after 7,15, 30 and 60 days of fructose consumption. Fructose consumption reduced baroreflex sensitivity by day 7, and still induced a progressive reduction in baroreflex sensitivity over the time. Fructose consumption also increased TNF alpha and IL-6 levels in the adipose tissue and IL-1 beta(3 levels in the spleen at days 15 and 30. Fructose consumption also reduced plasmatic nitrites (day 15 and 30) and superoxide dismutase activity (day 15 and 60), but increased hydrogen peroxide (day 30 and 60), lipid peroxidation and protein oxidation (day 60). Fructose consumption increased arterial pressure at day 30 (8%) and 60 (11%). Fructose consumption also induced a late insulin resistance at day 60, but did not affect glucose levels. In conclusion, the results show that baroreflex sensitivity impairment precedes inflammatory and oxidative stress disorders, probably by inducing hemodynamic and metabolic dysfunctions observed in metabolic syndrome.
- Cardiac and pulmonary arterial remodeling after sinoaortic denervation in normotensive rats(2012) FLUES, K.; MORAES-SILVA, I. C.; MOSTARDA, C.; SOUZA, P. R. M.; DINIZ, G. P.; MOREIRA, E. D.; PIRATELLO, A. C.; CHAVES, M. L. Barreto; ANGELIS, K. De; SALEMI, Vera Maria Cury; IRIGOYEN, M. C.; CALDINI, E. G.Blood pressure variability (BPV) and baroreflex dysfunction may contribute to end-organ damage process. We investigated the effects of baroreceptor deficit (10 weeks after sinoaortic denervation - SAD) on hemodynamic alterations, cardiac and pulmonary remodeling. Cardiac function and morphology of male Wistar intact rats (C) and SAD rats (SAD) (n = 8/group) were assessed by echocardiography and collagen quantification. BP was directly recorded. Ventricular hypertrophy was quantified by the ratio of left ventricular weight (LVW) and right ventricular weight (RVW) to body weight (BW). BPV was quantified in the time and frequency domains. The atrial natriuretic peptide (ANP), alpha-skeletal actin (alpha-skelectal), collagen type I and type III genes mRNA expression were evaluated by RT-PCR. SAD did not change BP, but increased BPV (11 +/- 0.49 vs. 5 +/- 0.3 mm Hg). As expected, baroreflex was reduced in SAD. Pulmonary artery acceleration time was reduced in SAD. In addition, SAD impaired diastolic function in both LV (6.8 +/- 0.26 vs. 5.02 +/- 0.21 mm Hg) and RV (5.1 +/- 0.21 vs. 4.2 +/- 0.12 mm Hg). SAD increased LVW/BW in 9% and RVW/BW in 20%, and augmented total collagen (3.8-fold in LV, 2.7-fold in RV, and 3.35-fold in pulmonary artery). Also, SAD increased type I (similar to 6-fold) and III (similar to 5-fold) collagen gene expression. Denervation increased ANP expression in LV (75%), in RV (74%) and increased a-skelectal expression in LV (300%) and in RV (546%). Baroreflex function impairment by SAD, despite not changing BP, induced important adjustments in cardiac structure and pulmonary hypertension. These changes may indicate that isolated baroreflex dysfunction can modulate target tissue damage.
- EFFECT OF SUPPLEMENTATION OF FRUIT EXTRACT (CRANBERRRY, BLUEBERRY AND POMEGRANATE) ON INSULIN RESISTANCE AND OXIDATIVE STRESS IN HYPERTENSIVE PATIENTS(2018) GAETA, L. N. N.; MORAES, M. C.; KATAYAMA, K. Y.; SANGALETTI, C. T.; IRIGOYEN, M. C.; FREITAS, S.; VIANA, A.; ANGELIS, K. De; LOPES, H. F.; CALDINI, Elia C.
- Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats(2020) MOREIRA, Nathalia Juocys Dias; SANTOS, Fernando dos; MOREIRA, Edson Dias; FARAH, Daniela; SOUZA, Leandro Eziquiel de; SILVA, Maikon Barbosa da; MORAES-SILVA, Ivana Cinthya; LINCEVICIUS, Gisele Silverio; CALDINI, Elia Garcia; IRIGOYEN, Maria Claudia CostaMechanisms involved in the acute responses to renal denervation (RDN) have yet to be fully understood. We assessed urinary volume, autonomic control and aorta vascular reactivity after acute RDN. Male normotensive Wistar rats and spontaneously hypertensive rats (SHR) were divided into normotensive+RDN (ND) or sham surgery (NS), and hypertensive+RDN (HD) or sham surgery (HS). Metabolic parameters and hemodynamic measurements were recorded 72h and 4 days after intervention, respectively. Aortic rings were studied 7 days post RDN in an isometric myograph. Concentration-response curves to phenylephrine, sodium nitroprusside and acetylcholine (10(-10)-10(-5) M) were performed. Two-way ANOVA was used for group comparisons and differences reported when p < 0.05. Results are presented as mean +/- SEM. Urinary volume was 112% higher in HD vs. HS (HS=14.94 +/- 2.5 mL; HD=31.69 +/- 2.2 mL) and remained unchanged in normotensive rats. Systolic BP was lower in HD rats (HS=201 +/- 12 vs. HD=172 +/- 3 mmHg) without changes in normotensive group. HD group showed increased HF and LF modulation (HS=5.8 +/- 0.7 ms(2) vs. HD=13.4 +/- 1.4 ms(2); HS=3.5 +/- 0.7 ms(2) vs. HD=10.5 +/- 1.7 ms(2), respectively). RDN normalized vascular reactivity in HD rats and increased phenylephrine response in ND rats. Acute fall in BP induced by RDN is associated with increased urinary volume, which in turn may also have contributed to functional changes of the aorta.
- Nicotinamide attenuates streptozotocin-induced diabetes complications and increases survival rate in rats: role of autonomic nervous system(2021) CRUZ, Paula L.; MORAES-SILVA, Ivana C.; RIBEIRO, Amanda A.; MACHI, Jacqueline F.; MELO, Marcelo Dantas Tavares de; SANTOS, Fernando dos; SILVA, Maikon Barbosa da; STRUNZ, Celia Maria Cassaro; CALDINI, Elia Garcia; IRIGOYEN, Maria-ClaudiaBackground To evaluate the effect of nicotinamide prior to streptozotocin-induced (STZ) diabetes in baroreflex sensitivity and cardiovascular autonomic modulation, and its association with hemodynamics and metabolic parameters. Methods Methods: Male Wistar rats were divided into control (Cont) and STZ-induced diabetes (Diab). Half of the rats from each group received a single dose of nicotinamide (100 mg/Kg) before STZ injection (Cont+NicA and Diab+NicA). All groups were followed-up for 5 weeks. Results Body weight loss of more than 40% was observed in Diab throughout the period (Diab: 271.00 +/- 12.74 g; Diab+NicA: 344.62 +/- 17.82). Increased glycemia was seen in Diab rats (541.28 +/- 18.68 mg/dl) while Diab+NicA group had a slight decrease (440.87 +/- 20.96 mg/dl). However, insulin resistance was observed only in Diab. In relation to Cont, heart rate, mean blood pressure and diastolic function were reduced when compared to Diab, together with parasympathetic modulation and baroreflex sensitivity. All of these parameters were improved in Diab+NicA when compared to Diab. Improved baroreflex sensitivity and parasympathetic modulation were correlated with glycemia, insulin resistance, and body weight mass. Additionally, Diab+NicA group increased survival rate. Conclusions Results suggest that the association of nicotinamide in STZ-induced diabetic rats prevents most of the expected derangements mainly by preserving parasympathetic and baroreflex parameters.
conferenceObject Exercise Training Improves High Blood Pressure Variability-induced Cardiac Damage In Normotensive Rats(2020) MORAES-SILVA, Ivana C.; ANGELIS, Katia De; DAMACENO-RODRIGUES, Nilsa R.; CALDINI, Elia G.; IRIGOYEN, Maria Claudia