ELIA TAMASO ESPIN GARCIA CALZOLARI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
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- Myofibroblasts are increased in the lung parenchyma in asthma(2017) BOSER, Stacey R.; MAUAD, Thais; ARAUJO-PAULINO, Bianca Bergamo de; MITCHELL, Ian; SHRESTHA, Grishma; CHIU, Andrea; BUTT, John; KELLY, Margaret M.; CALDINI, Elia; JAMES, Alan; GREEN, Francis H. Y.Background Increased airway smooth muscle is observed in large and small airways in asthma. Semi-quantitative estimates suggest that cells containing alpha smooth muscle actin (alpha-SMA) are also increased in the lung parenchyma. This study quantified and characterized alpha-SMA positive cells (alpha-SMA+) in the lung parenchyma of non-asthmatic and asthmatic individuals. Methods Post-mortem sections of peripheral lung from cases of fatal asthma (FA), persons with asthma dying of non-respiratory causes (NFA) and non-asthma control subjects (NAC) were stained for alpha-SMA, quantified using point-counting and normalised to alveolar basement membrane length and interstitial area. Results alpha-SMA+ fractional area was increased in alveolar parenchyma in both FA (14.7 +/- 2.8% of tissue area) and NFA (13.0 +/- 1.2%), compared with NAC (7.4 +/- 2.4%), p < 0.05 The difference was greater in upper lobes compared with lower lobes (p < 0.01) in both asthma groups. Similar changes were observed in alveolar ducts and alveolar walls. The electron microscopic features of the alpha-SMA+ cells were characteristic of myofibroblasts. Conclusions We conclude that in asthma there is a marked increase in alpha-SMA+ myofibroblasts in the lung parenchyma. The physiologic consequences of this increase are unknown.