DENISE AYA OTSUKI

(Fonte: Lattes)
Índice h a partir de 2011
11
Lattes
ResearcherID
ORCID
Projetos de Pesquisa
Unidades Organizacionais
LIM/08 - Laboratório de Anestesiologia, Hospital das Clínicas, Faculdade de Medicina

Filtros

Limpar filtros

Configurações

Colaboração internacional: Chile ×

Resultados de Busca

Agora exibindo 1 - 1 de 1
  • article 8 Citação(ões) na Scopus
    Nebulization of Vancomycin Provides Higher Lung Tissue Concentrations than Intravenous Administration in Ventilated Female Piglets with Healthy Lungs
    (2020) MORAIS, Cristiane Luchesi de Mello; NASCIMENTO, Jorge Willian Leandro; RIBEIRO, Aline Correa; CORTINEZ, Luis Ignacio; CARMONA, Maria Jose Carvalho; MAIA, Debora Rothstein Ramos; MONSEL, Antoine; AULER JR., Jose Otavio Costa; ROUBY, Jean-Jacques; OTSUKI, Denise Aya
    Background: Intravenous vancomycin is used to treat ventilator-associated pneumonia caused by methicillin-resistant Staphylococcus aureus, but achieves high rates of failure. Vancomycin nebulization may be efficient to provide high vancomycin lung tissue concentrations. The aim of this study was to compare lung tissue and serum concentrations of vancomycin administered intravenously and by aerosol in mechanically ventilated and anesthetized healthy piglets. Methods: Twelve female piglets received a single intravenous dose of vancomycin (15mg/kg) and were killed 1 (n = 6) or 12h (n = 6) after the end of administration. Twelve piglets received a single nebulized dose of vancomycin (37.5mg/kg) and were killed 1 (n = 6) or 12h (n = 6) after the end of the aerosol administration. In each group, vancomycin lung tissue concentrations were assessed on postmortem lung specimens using high-performance liquid chromatography. Blood samples were collected for serum vancomycin concentration measurement 30min and 1, 2, 4, 6, 8, and 12h after the end of vancomycin administration. Pharmacokinetics was analyzed by nonlinear mixed effect modeling. Results: One hour after vancomycin administration, lung tissue concentrations in the aerosol group were 13 times the concentrations in the intravenous group (median and interquartile range: 161 [71, 301] mu g/g versus 12 [4, 42] mu g/g; P < 0.0001). Twelve hours after vancomycin administration, lung tissue concentrations in the aerosol group were 63 (23, 119) mu g/g and 0 (0, 19) mu g/g in the intravenous group (P < 0.0001). A two-compartment weight-scaled allometric model with first-order absorption and elimination best fit serum pharmacokinetics after both routes of administration. Area under the time-concentration curve from 0 to 12 h was lower in the aerosol group in comparison to the intravenous group (56 [8, 70] mg . h . l(-1)vs. 121 [103, 149] mg . h . l(-1), P = 0.002). Using a population model, vancomycin bioavailability was 13% (95% CI, 6 to 69; coefficient of variation = 85%) and absorption rate was slow (absorption half life = 0.3h). Conclusions: Administration of vancomycin by nebulization resulted in higher lung tissue concentrations than the intravenous route.