FLAVIA REGINA ROTEA MANGONE
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Cocaine use disorder effects on blood oxytocin levels and OXTR DNA methylation(2023) SOUZA, Manasses Soares; SANVICENTE-VIEIRA, Breno; ZAPARTE, Aline; BAPTISTA, Talita; NAGAI, Maria Aparecida; MANGONE, Flavia Rotea; PAVANELLI, Ana Carolina; VIOLA, Thiago Wendt; GRASSI-OLIVEIRA, RodrigoSubstance use disorders have been associated with alterations in the oxytocinergic system, but few studies have investigated both the peptide and epigenetic mechanisms potentially implicated in the regulation of oxytocin receptor. In this study, we compared plasma oxytocin and blood DNA methylation in the OXTR gene between people with and without cocaine use disorder (CUD). We measured the oxytocin levels of 51 people with CUD during acute abstinence and of 30 healthy controls using an enzyme immunoassay. The levels of DNA methylation in four CpG sites at exon III of the OXTR gene were evaluated in a subsample using pyrosequencing. The Addiction Severity Index was used to assess clinical characteristics. We found higher oxytocin levels in men with CUD (56.5 pg/mL; 95% CI: 48.2-64.7) than in control men (33.6 pg/mL; 95% CI: 20.7-46.5), while no differences between women with and without CUD were detected. With a moderate effect size, the interaction effect between group and sex remained significant when controlling for height, weight and age data. A positive correlation in the CUD sample was found between oxytocin levels and days of psychological suffering prior to treatment enrollment. No group differences were observed regarding DNA methylation data. This suggests that CUD is associated with higher peripheral oxytocin levels in men during acute abstinence. This finding may be considered in future studies that aim at using exogenous oxytocin as a potential treatment for cocaine addiction.
- Interrelated but Not Time-Aligned Response in Myogenic Regulatory Factors Demethylation and mRNA Expression after Divergent Exercise Bouts(2023) TELLES, Guilherme Defante; LIBARDI, Cleiton Augusto; CONCEICAO, Miguel Soares; VECHIN, Felipe Cassaro; LIXANDRAO, Manoel Emilio; MANGONE, Flavia Regina Rotea; PAVANELLI, Ana Carolina; NAGAI, Maria Aparecida; CAMERA, Donny Michael; HAWLEY, John A.; UGRINOWITSCH, CarlosIntroductionDNA methylation regulates exercise-induced changes in the skeletal muscle transcriptome. However, the specificity and the time course responses in the myogenic regulatory factors DNA methylation and mRNA expression after divergent exercise modes are unknown.PurposeThis study aimed to compare the time course changes in DNA methylation and mRNA expression for selected myogenic regulatory factors (MYOD1, MYF5, and MYF6) immediately after, 4 h after, and 8 h after a single bout of resistance exercise (RE), high-intensity interval exercise (HIIE), and concurrent exercise (CE).MethodsNine healthy but untrained males (age, 23.9 +/- 2.8 yr; body mass, 70.1 +/- 14.9 kg; peak oxygen uptake [V?O-2peak], 41.4 +/- 5.2 mL center dot kg(-1)center dot min(-1); mean +/- SD) performed a counterbalanced, randomized order of RE (4 x 8-12 repetition maximum), HIIE (12 x 1 min sprints at V?O-2peak running velocity), and CE (RE followed by HIIE). Skeletal muscle biopsies (vastus lateralis) were taken before (REST) immediately (0 h), 4 h, and 8 h after each exercise bout.ResultsCompared with REST, MYOD1, MYF5, and MYF6, mean methylation across all CpGs analyzed was reduced after 4 and 8 h in response to all exercise protocols (P < 0.05). Reduced levels of MYOD1 methylation were observed after HIIE and CE compared with RE (P < 0.05). Compared with REST, all exercise bouts increased mRNA expression over time (MYOD1 at 4 and 8 h, and MYF6 at 4 h; P < 0.05). MYF5 mRNA expression was lower after 4 h compared with 0 h and higher at 8 h compared with 4 h (P < 0.05).ConclusionsWe observed an interrelated but not time-aligned response between the exercise-induced changes in myogenic regulatory factors demethylation and mRNA expression after divergent exercise modes. Despite divergent contractile stimuli, changes in DNA methylation and mRNA expression in skeletal muscle were largely confined to the late (4-8 h) recovery period and similar between the different exercise challenges.