KATIA CANDIDO CARVALHO
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Differential BMP4 and GREM1 protein expression in uterine leiomyosarcoma and leiomyoma(2013) GARCIA, Natalia; SOUZA, Faila Catarina; CUNHA, Isabela Werneck; SOARES, Fernando Augusto; MACIEL, Gustavo Arantes Rosa; BARACAT, Edmund Chada; CARVALHO, Katia Candido
- Metoclopramide-induced hyperprolactinemia effects on the pituitary and uterine prolactin receptor expression(2013) AMARAL, Vinicius C.; MACIEL, Gustavo A. R.; CARVALHO, Katia C.; MARCONDES, Rodrigo R.; SOARES JR., Jose Maria; BARACAT, Edmund C.In this work we have evaluated the gene expression profile of prolactin and prolactin receptor in the pituitary and the uterus of female mice with metoclopramide-induced hyperprolactinemia treated with estrogen and/or progesterone. For this purpose, 49 Swiss female mice were allocated to seven groups. Interventions: 50-day treatment with metoclopramide, progesterone and estrogen. Our results showed that in the pituitary, metoclopramide-induced hyperprolactinemia increased prolactin expression. In the castrated animals, progesterone, with or without estrogen, produced an increase in prolactin. Pituitary prolactin receptor and the estrogen and progesterone treatment were responsible for the rise in PRLR-S2. In the uterus, no differences in prolactin expression were found between the different study groups. PRLR-S1 had its expression reduced in all castrated animals as against the castrated group treated with vehicle. In the noncastrated animals, PRLR-52 rose in the metoclopramide-treated group, and, in the castrated animals, its expression diminished in all groups in relation to the vehicle-treated castrated controls. An increase in PRLR-S3 was found in the oophorectomized animals treated with a combination of estrogen and progesterone. PRLR-L rose in the oophorectomized animals treated with progesterone in isolation or in association with estrogen. These findings suggest that metoclopramide associated to progesterone or estrogen may determine an increase in pituitary prolactin and PRLR-S2 expression. The estrogen-progesterone may enhance the expression of PRLR-S3 and PRLR-L isoform of prolactin receptor.
- Cell cycle suppressor proteins are not related to HPV status or clinical outcome in patients with vulvar carcinoma(2013) LAVORATO-ROCHA, Andre Mourao; RODRIGUES, Iara Sant'ana; MAIA, Beatriz de Melo; STIEPCICH, Monica Maria Agata; BAIOCCHI, Glauco; CARVALHO, Katia Candido; SOARES, Fernando Augusto; VASSALLO, Jose; ROCHA, Rafael MalagoliInteractions between the cyclin-dependent kinase inhibitors (CDKI) and human papillomavirus (HPV) infection in the pathogenesis of vulvar carcinoma are still incomplete. This study aimed to evaluate the prognostic relevance of these proteins in vulvar cancer. One hundred and thirty-nine patient specimens assembled in a tissue microarray were evaluated for p16, p21, p27, and pRb by immunohistochemistry. HPV status was assessed by a linear array HPV genotyping test. In 16 cases with available frozen tumor, quantitative real-time reverse transcriptase-polymerase chain reaction for CDKN2A(p16), CDKN1A, and Rb was performed. Protein expression was considered positive in 40 patients for p16, 35 for p21, 28 for p27, and 19 for pRb. HPV was positive in 43 of the 105 evaluable cases. Expression of CDKIs and pRb, with the exception of p16, seem to be linked to the early phases of vulvar carcinogenesis. Although p16 and p21 protein expression was associated with early stages of disease, no prognostic significance was found when analyzing CDKI proteins or detecting HPV status, limiting their clinical usage. No association was observed between expression of CDKI proteins and HPV status, suggesting that in spite of this association found in cervical cancer, this seems not to be valid for vulvar carcinoma.
conferenceObject Polymorphisms of estrogen receptor-alpha gene in Brazilian women with high breast density after menopause(2013) SOUZA, M.; FONSECA, A.; BAGNOLI, V; HORTENSE, V. H.; SOARES, J. M.; CARVALHO, K.; BARACAT, E.- CD9, CD63 and CD82 tetraspanins expression profile in vulvar carcinomas(2013) CARVALHO, Katia C.; ROCHA, Rafael M.; CUNHA, Isabela W.; SOARES, Fernando A.
- Prognostication of Vulvar Cancer Based on p14(ARF) Status: Molecular Assessment of Transcript and Protein(2013) LAVORATO-ROCHA, Andre Mourao; MAIA, Beatriz de Melo; RODRIGUES, Iara Sant'ana; STIEPCICH, Monica Maria Agata; BAIOCCHI, Glauco; CESTARI, Flavia Munhoz da Silva; CARVALHO, Katia Candido; SOARES, Fernando Augusto; ROCHA, Rafael MalagoliThis study was designed to determine the prognostic role of p14(ARF) in vulvar squamous cell carcinoma (VSCC). Immunohistochemistry for p14(ARF) and p53 and fluorescent in situ hybridization (FISH) for TP53 were performed in 139 cases of VSCC. Human papillomavirus (HPV) genotyping by hybridization was employed in 100 cases. qRT-PCR for p14(ARF) and p53 transcript assessment was performed in 16 cases. All results were correlated with clinicopathological variables. Immunohistochemistry analysis showed p14(ARF) and p53 positivity in 16.4 % and 53 % cases respectively. Positive p14(ARF) expression was significantly associated with the following variables: shorter cancer-specific survival (P = 0.04) and shorter disease-free survival (P = 0.02), presence of perineural invasion (P = 0.037), vascular invasion (P = 0.047), and node metastasis (P = 0.031). Also, p14(ARF)-positive HPV-negative cases had the shortest cancer-specific survival (P = 0.03) and disease-free survival (P = 0.04). HPV infection was detected in 32.8 % of the cases; HPV16 was the most prevalent type. Viral infection was more common in poorly differentiated tumors (P = 0.032). qRT-PCR demonstrated that CDKN2A (p14(ARF)) had higher expression in tumor samples compared with paired noncancerous samples (P < 0.001). The opposite relationship was seen in TP53 expression evaluation (P < 0.001). FISH demonstrated 4 cases with deleted TP53 (6.3 %). p14(ARF) represents an important marker of poor prognosis in VSCC. p53 and HPV infection did not show any prognostic importance. Further clinical trials concerning p14(ARF) positivity may result in important contributions due to its relationship with poor outcome. Mainly due to the relationship of p14(ARF) with lymph node metastasis, the immunohistochemistry evaluation of this marker may help to identify a subset of patients more suitable to less radical procedures.
- Polymorphisms of estrogen receptor-alpha gene in Brazilian women with high breast density after menopause(2013) SOUZA, Marilene Alicia; FONSECA, Angela Maggio; BAGNOLI, Vicente R.; BARROS, Nestor de; FRANZOLIN, Solange O. B.; CARVALHO, Katia C.; SOARES- JR., Jose Maria; BARACAT, Edmund C.The association of genetic polymorphism in the estrogen receptor alpha (ER alpha) gene and risk for diseases including breast cancer (BC) has been the subject of great interest. Objective: Checking on women with high breast density after menopause, the frequency of the Pvull and Xbal polymorphisms of the ER alpha gene and the correlation between them and the known risk factors for breast cancer. Method: Observational study with 308 women between 45 and 65 years old with high breast density, without hormonal therapy, menstruation for a year or more, breast and ovarian cancer history. It was characterized in clinical history and physical examination: menarche, menopause, parity, family history of BC, smoking, alcohol intake and body mass index. Results: The allelic and genotypic frequencies for ER alpha-Pvull and Xbal: p = 43.99%; p = 56.01%; pp = 32.14%; Pp = 47.73% and PP = 20.13%; X = 41.56%; x = 58.44%; xx = 33.44%; Xx = 50.00% and XX = 16.56%, respectively. The most frequent risk factors for BC: menarche before 12 years old (35.38%), nulliparity or first child after 28 years old (41.66%), family history of BC (19.16%) and overweight/obesity (62.01%). Conclusion: Allelic and genotypic distribution similar to literature. The risk factors for BC were more prevalent in women with high breast density but without significant associations with these polymorphisms.