KATIA CANDIDO CARVALHO
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
- Granulosa cells and follicular development: a brief review(2023) CAVALCANTI, Giovanna Santos; CARVALHO, Katia Candido; FERREIRA, Cecilia da Silva; ALVAREZ, Peter Andre Chedraui; MONTELEONE, Pedro Augusto Araujo; BARACAT, Edmund Chada; JR, Jose Maria Soares
- Could be FOXO3a, miR-96-5p and miR-182-5p useful for Brazilian women with luminal A and triple negative breast cancers prognosis and target therapy?(2023) MENDES, Daniele Carvalho Calvano; CALVANO FILHO, Carlos Marino Cabral; GARCIA, Natalia; RICCI, Marcos Desiderio; SOARES JUNIOR, Jose Maria; CARVALHO, Katia Candido; BARACAT, Edmund ChadaFOXO3a dysregulation is frequently implicated in tumorigenesis, and its inhibition can occur by several molecular mechanisms. Among these, post-transcriptional suppression by miRNAs has been associated with various cancers initiation. Here, we assessed the expression profiles of the most relevant miRNAs for breast tumorigenesis, using Luminal A (LA) and Triple-Negative (TN) breast cancer from Brazilian patients, by the quantitative real time-PCR method. Their potential prognostic role for the patients was also evaluated. We identified the miRNAs miR-96-5p and miR-182-5p, de-scribed as negative regulators of FOXO3A, with differential expression both in LA and TN tumors when compared to normal tissue. The miR-96-5p and miR-182-5p miRNAs were upregulated in LA (7.82 times, p < 0.005; 6.12 times, p < 0.005, respectively) and TN breast cancer samples (9.42 times, p < 0.0001; 8.51 times, p < 0.0001) compared to normal tissues. The samples with higher miR-96-5p and miR-182-5p expression (FR >= 4) were submitted for FOXO3a immunostaining. Reduced protein detection was observed in all of the tumors compared to normal tissues. The most prominent miRNA expression and FOXO3a protein suppres-sion were observed in TN samples (p < 0.001), indicating the relevant role of these molecules in this tumor biol-ogy and clinical behavior. Our results corroborate the literature regarding to the relevance of FOXO3a in the breast cancer, and they open new perspectives for alternative target therapy options for Brazilian patients express-ing both FOXO3a and its regulatory miRNAs.
- Granulosa cells and follicular development: a brief review (vol 69, e20230175, 2023)(2023) CAVALCANTI, Giovanna Santos; CARVALHO, Katia Candido; FERREIRA, Cecilia da Silva; CHEDRAUI, Peter; MONTELEONE, Pedro Augusto Araujo; BARACAT, Edmund Chada; SOARES JUNIOR, Jose Maria