FLAVIO CARNEIRO HOJAIJ
Projetos de Pesquisa
Unidades Organizacionais
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina
10 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 10
conferenceObject Non-ablative radiofrequency enhance collagen content on the injured Achilles tendon after one year in rats(2017) AKAMATSU, Flavia Emi; ITEZEROTE, Ana Maria; SALEH, Samir; HOJAIJ, Flavio; ANDRADE, Mauro; TEODORO, WalcyconferenceObject Experimental model of Achilles tendon injury in rats(2014) AKAMATSU, Flavia; TEODORO, Walcy; SALEH, Samir; SILVA, Alexandre; HOJAIJ, Flavio; MARTINEZ, Carlos; ANDRADE, Mauro; JACOMO, Alfredo- Photobiomodulation therapy increases collagen II after tendon experimental injury(2021) AKAMATSU, Flayia Emi; TEODORO, Walcy Rosolia; ITEZEROTE, Ana Maria; SILVEIRA, Lizandre Keren Ramos da; SALEH, Samir; MARTINEZ, Carlos Augusto Real; RIBEIRO, Marcelo Lima; PEREIRA, Jose Aires; HOJAIJ, Flavio; ANDRADE, Mauro; JACOMO, Alfredo LuizA tendon is a mechanosensitive tissue that transmits muscle-derived forces to bones. Photobiomodulation (PBM), also known as low-level laser therapy (LLLT), has been used in therapeutic approaches in tendon lesions, but uncertainties regarding its mechanisms of action have prevented its widespread use. We investigated the response of PBM therapy in experimental lesions of the Achilles tendon in rats. Thirty adult male Wistar rats weighing 250 to 300 g were surgically submitted to bilateral partial transverse section of the Achilles tendon. The right tendon was treated with PBM, whereas the left tendon served as a control. On the third postoperative day, the rats were divided into three experimental groups consisting of ten rats each, which were treated with PBM (Konf, Aculas HB 750), 780 nm and 80 mW for 20 seconds, three times/week for 7, 14 and 28 days. The rats were sacrificed at the end of the therapeutic time period. The Sca-1 was examined by immunohistochemistry and histomorphometry, and COLA1, COLA2 and COLA3 gene expression was examined by qRT-PCR. COLA2 gene expression was higher in PBM treated tendons than in the control group. The histomorphometric analysis coincided with increased number of mesenchymal cells, characterized by Sca-1 expression in the lesion region (p<0.001). PBM effectively interferes in tendon tissue repair after injury by stimulating mesenchymal cell proliferation and the synthesis of collagen type II, which is suggested to provide structural support to the interstitial tissues during the healing process of the Achilles tendon. Further studies are needed to confirm the role of PBM in tendon healing.
conferenceObject Radiofrequency promotes more collagen synthesis than low level laser in experimental tendineous lesion(2014) AKAMATSU, Flavia; TEODORO, Walcy; SALEH, Samir; HOJAIJ, Flavio; CAMILLO, Gina; MARTINEZ, Carlos; ANDRADE, Mauro; JACOMO, Alfredo- Is the colon mucosa affected by ten days of gastric restriction in an animal model?(2019) AKAMATSU, Flavia Emi; FONTES, Luiz Gustavo; ITEZEROTE, Ana Maria; SALEH, Samir; TEODORO, Walcy Paganelli Rosolia; ARTIFON, Everson; HOJAIJ, Flavio; ANDRADE, Mauro; PEREIRA, Jose Aires; MARTINEZ, Carlos Augusto Real; JACOMO, Alfredo LuizPurpose: To identify whether the colon mucosa is affected by ten days of gastric restriction in an animal model. Methods: An experimental model of gastric restriction was devised using rats. The animals were submitted to surgical gastrostomy, and a cylindrical loofah was inserted into the stomach. We studied 30 adult male Wistar rats divided into three groups: the stomach restriction group (R10); the sham group (S10), which underwent the same procedure except for the loofah insertion; and the control group (C10). The expression of neutral and acid mucins was evaluated using histochemical techniques. Goblet cells and protein content were compared between groups using generalized estimation equations (GEEs). Bonferroni's multiple comparison was applied to identify differences between the groups. All tests considered a 5% significance level. Results: There was an increased expression of neutral mucins, acid mucins and goblet cells in the R10 group. Collagen was also enhanced in the R10 group. Conclusion: The colon mucosa is affected by ten days of gastric restriction in an animal model, increasing neutral mucins, acid mucins and collagen content with trophic maintenance.
conferenceObject COLIIA1 overexpression with low level laser therapy after experimental injury(2014) JACOMO, Alfredo; TEODORO, Walcy; SALEH, Samir; MARTINEZ, Carlos; RIBEIRO, Marcelo; HOJAIJ, Flavio; ANDRADE, Mauro; AKAMATSU, FlaviaconferenceObject Non-ablative radiofrequency enhance collagen content on the injured Achilles tendon after one year in rats(2017) AKAMATSU, Flavia Emi; ITEZEROTE, Ana Maria; SALEH, Samir; HOJAIJ, Flavio; ANDRADE, Mauro; TEODORO, Walcyarticle 2 Citação(ões) na Scopus Radiofrequency preserves histoarchitecture and enhances collagen synthesis in experimental tendon injury(2016) AKAMATSU, Flavia Emi; SALEH, Samir Omar; HOJAIJ, Flavio; AUGUSTO, Carlos; MARTINEZ, Real; ANDRADE, Mauro; TEODORO, Walcy Rosolia; JACOMO, Alfredo LuizWe investigated the action of radiofrequency (RF) on the healing process after inducing experimental lesions of the Achilles tendon in rats. Wistar rats were surgically subjected to bilateral partial transverse sectioning of the Achilles tendon. The right tendon was treated with radiofrequency (RFT), whereas the left tendon served as a control (CT). On the third postoperative day, the rats were divided into three experimental groups consisting of ten rats each, which were treated with monopolar radiofrequency (Tonederm (TM)) until they were sacrificed on the 7th, 14th or 28th days. The histological specimens were studied for inflammatory cell content, collagen types I and III, immunostaining and morphometry. Total collagen were biochemically analyzed and to evalute fibroblast and myofibroblast proliferation by vimentin and a-actin smooth muscle immunohistochemistry methods. Statistical analysis was performed using the Student's t-test, the sign test and the Kruskal-Wallis test to compare tendons treated with radiofrequency with the non-treated tendons (alpha=5%; alpha=10%). Larger amounts of collagen I with hydroxyproline content and myofibroblast cells were clearly evident within 7 days (p<0.05). No difference was observed in the inflammatory cell content between the groups. We found better collagen arrangement with RF administration across the entire time studied. Radiofrequency administration preserves histoarchitecture and enhances collagen synthesis during the initial phases of cicatrization, suggesting that the treatment can provide improved stiffness during the most vulnerable phases of tendon healing. Clinical studies may include RF among the therapeutic tools in tendinous lesion management.conferenceObject LOW LEVEL LASER THERAPY INCREASES MESENCHYMAL CELL PROLIFERATION AND COLLAGEN II AFTER EXPERIMENTAL INJURY(2018) AKAMATSU, Flavia Emi; TEODORO, Walcy R.; ITEZEROTE, Ana Maria; SALEH, Samir; MARTINEZ, Carlos Augusto Real; RIBEIRO, Marcelo Lima; PEREIRA, Jose Aires; HOJAIJ, Flavio; ANDRADE, Mauro; JACOMO, Alfredo LuizconferenceObject Tissue quantification of neutral and acid mucins in the mucosa of the colon submitted to 10 days of gastric restriction in an animal model(2019) AKAMATSU, Flavia Emi; FONTES, Luiz Gustavo; ITEZEROTE, Ana Maria; SALEH, Samir; TEODORO, Walcy Rosolia; HOJAIJ, Flavio; ANDRADE, Mauro; PEREIRA, Jose Aires; MARTINEZ, Carlos Augusto Real; JACOMO, Alfredo Luiz