HEITOR FRANCO DE ANDRADE JUNIOR
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
- TOXOPLASMA GONDII INFECTION OF CHICKEN EMBRYOS CAUSES RETINAL CHANGES AND MODULATES HSP90B1 GENE EXPRESSION: A PROMISING OCULAR TOXOPLASMOSIS MODEL(2015) NASARE, Alex M.; TEDESCO, Roberto C.; CRISTOVAM, Priscila C.; CENEDESE, Marcos A.; GALISTEO JR., Andres J.; ANDRADE JR., Heitor F.; GOMES, Jose Alvaro P.; GUIMARAES, Erik V.; BARBOSA, Helene S.; ALONSO, Luis G.HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos' eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny.
- Dermal Dendrocytes FXIIIa plus Are Essential Antigen-Presenting Cells in Indeterminate Leprosy(2015) LIRA, Marcia Lanzoni de Alvarenga; PAGLIARI, Carla; SILVA, Aline Alves de Lima; ANDRADE JR., Heitor Franco de; DUARTE, Maria Irma SeixasIndeterminate leprosy (IL) is the early phase of Hansen disease and reword (APCs). Langerhans cells and dermal dendrocytes FXIIIa positive (DDFXIIIa) are the major APCs in the skin and can be identified by the expression of CD1a and FXIIIa, respectively, by immunohistochemical techniques. Plasmacytoid dendritic cells (PDCs) are another type of dermal dendrocytes with a questionable antigen-presenting function and can be highlighted by anti-CD123 expression. To our knowledge, there are no studies evaluating DDFXIIIa and PDC in IL. The purpose was to investigate the involvement of these cells in the pathogenesis of IL. The authors performed a retrospective study on 18 cases of IL (10 confirmed and 8 suspected) to investigate expression of FXIIIa, CD1a, and CD123. The results were compared with normal skin (for CD1a and FXIIIa only). A higher amount of FXIIIa-positive cells (P < 0.05) in confirmed and suspected IL cases was noted when comparing with normal skin. However, CD1a showed no quantitative differences in the epidermis of IL lesions when comparing with normal skin and CD123 expression was negligible. Based on these findings, the authors postulate that Langerhans cells and PDCs do not have a major role in IL and that DDFXIIIa may be the main APCs in IL. Further study is required to establish this.
- Efficient duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of specific anti-T. gondii IgG and IgM due to refined conjugates(2015) RODRIGUES, J. P.; ANDRADE JR., H. F.Toxoplasma gondii infections are very common, causing occasional central nervous system and eye diseases, and must be screened in prenatal care for efficient therapy. Here, we developed a duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of anti-T. gondii IgG and IgM antibodies in prenatal care screening for toxoplasmosis. Assays using commercially available ion-exchange purified conjugates yielded poor results and high background fluorescence. Same-well IgG/IgM dFISA with refined conjugates was used to test 140 samples from university students, 120 samples from pregnant women and 24 samples from adult volunteers at a large public hospital. We found that dFISA offers high concordance, specificity and reproducibility for IgG (Kappa = 0.883) and IgM (Kappa = 0.918), which is useful in high-throughput applications for antenatal care.
conferenceObject IGG PRODUCTION, CIRCULATING IMUNE COMPLEXES AND IGG AVIDITY MATURATION DURING EXPERIMENTAL HAMSTER VISCERAL LEISHMANIASIS(2015) CARVALHO, Camila Aparecida de; FERRAO, Thiago Fidelis; ANDRADE JR., Heitor Franco de- HUMAN TOXOPLASMOSIS OUTBREAKS AND THE AGENT INFECTING FORM. FINDINGS FROM A SYSTEMATIC REVIEW(2015) MEIRELES, Luciana Regina; EKMAN, Claudio Cesar Jaguaribe; ANDRADE JR., Heitor Franco de; ALBUQUERQUE, Expedito Jose deToxoplasmosis, a worldwide highly prevalent zoonotic infection, is transmitted either by the oocysts, from water and soil, or the tissue cysts, in raw or undercooked infected meat, of Toxoplasma gondii. An ongoing debate is whether there are differences between the clinical and epidemiological characteristics of the outbreaks due to one or the other infective form of the agent. We performed a systematic review, recovering 437 reported outbreaks of which 38 were selected. They were complete reports containing ascribed Toxoplasma infecting form, and clinical and demographic data. There was no gender or age group selection in the outbreaks, which were described more often in the Americas. A large number of individuals were affected when oocysts, associated with soil and water contaminated with cat feces, were considered the transmission source. Onset of symptoms occurred early when the infection was ascribed to meat tissue cysts (11.4 +/- 6.7 days) with sharpened temporal distribution of cases, while a broader and prolonged appearance of new cases was observed when oocysts in water were the source of the infection (20 +/- 7 days, p < 0.001). Such information may be useful in the design and implementation of control strategies.
conferenceObject ANTI-TOXOPLASMA GONDII IGG DETECTION IN SALIVA FOR TOXOPLASMOSIS EPIDEMIOLOGICAL STUDIES(2015) SAMPAIO, Barbara F.; ANDRADE, Heitor Franco; MEIRELES, Luciana Regina- Seroepidemiological analysis of toxoplasmosis in college students(2015) RODRIGUES, Jaqueline Polizeli; FREI, Fernando; NAVARRO, Italmar Teodorico; SILVA, Luciana Pereira; MARCELINO, Monica Yonashiro; ANDRADE-JUNIOR, Heitor Franco de; FARIA, Carolina Arruda de; SANTOS, Marislene; RIBEIRO-PAES, Joao TadeuBackground: Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods: In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondii antibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results: The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion: In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.
conferenceObject PLASMODIUM CHABAUDI MALARIA IN MICE STRAINS PHENOTIPICALLY SELECTED FOR MAXIMAL OR MINIMAL ACUTE INFLAMMATORY RESPONSE(2015) MACEDO, Bruna; ANDRADE, Heitor Franco; HERMIDA, Felipe De Melo; FRANCO, Marcelo de Franco de; BORREGO, Andrea BorregoconferenceObject STANDARDIZATION OF SOLID-PHASE FLUORESCENT ASSAY FOR THE SIMULTANEOUS DETECTION OF SPECIFIC ANTI-TOXOPLASMA GONDII IGG, IGM AND IGA ANTIBODIES FOR TOXOPLASMOSIS ANTENATAL CARE SCREENING(2015) RODRIGUES, Jaqueline P.; ANDRADE JUNIOR, Heitor Franco