RICARDO HSIEH

(Fonte: Lattes)
Índice h a partir de 2011
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Lattes
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Departamento de Dermatologia, Faculdade de Medicina
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 2 de 2
  • article 0 Citação(ões) na Scopus
    Actionable Mutation Profile of Sun-Protected Melanomas in South America
    (2022) HSIEH, Ricardo; NICO, Marcello M. S.; CAMILLO, Claudia M. C.; OLIVEIRA, Katia K.; CARRARO, Dirce M.; SANGUEZA, Martin; V, Silvia Lourenco
    Melanomas that arise in sun-protected sites, including acral and oral mucosal melanomas, are likely under the control of unique, specific mechanisms that lead to mutagenesis through various pathways. In this study, we examined somatic mutations in tumors by targeted sequencing using a custom Ion Ampliseq Panel, comprising hotspots of 14 genes that are frequently mutated in solid tumors. Tumor DNA was extracted from 9 formalin fixation, paraffin-embedded sun-protected melanomas (4 primary oral mucosal melanomas and 5 acral lentiginous melanomas), and we identified mutations in the NRAS, PIK3CA, EGFR, HRAS, ERBB2, and ROSI genes. This study reveals new actionable mutations that are potential targets in the treatment of photo-protected melanomas. Additional studies on more of these melanoma subtypes could confirm our findings and identify new mutations.
  • article 3 Citação(ões) na Scopus
    Claudin expression is maintained in mucoepidermoid carcinoma of the salivary gland
    (2020) ARRUDA, Claudia Fabiana Joca de; COUTINHO-CAMILLO, Claudia Malheiros; MARQUES, Marcia Martins; NAGANO, Cibele Pidorodeski; BOLOGNA, Sheyla Batista; BETTIM, Barbara Beltrame; GERMANO, Janaina Naiara; PINTO, Clovis Antonio Lopes; HSIEH, Ricardo; LOURENCO, Silvia Vanessa
    Objective: The aim of the present study was to investigate the expression of claudin-1,-3,-4,-5 and-7 proteins in mucoepidermoid carcinoma of oral cavity and analyze whether EGF may interfere in the expression of the genes that encode claudins using in vitro models. Material and methods: Using immunohistochemistry, the expression of claudins was searched in 36 histologically graded cases of mucoepidermoid carcinoma. The association of expression of claudins with clinical-pathological parameters was evaluated. An in vitro step investigated the influence of EGF on gene expression of claudins by real time RT-PCR technique. Results: Claudin-1,-3,-4,-5, and-7 were highly expressed in most mucoepidermoid carcinomas. These expres-sions were compared with clinicopathological parameters. High expression of claudin-1 was associated with patients over 40 years-old (p = 0.05) and Caucasians (p = 0.024). In vitro experiments demonstrated a tendency for Claudin gene expression increase after EGF stimulus. Conclusions: The expression of claudins is maintained in mucoepidermoid carcinoma cells and EGF could be related with this expression. Our results point out to a fundamental biological importance to CLDNs in normal and neoplastic tissue. The expression patterns of CLDNs does not yet allow a clinical application, but the biological knowledge will ground evidence to new studies towards possible target-therapies.