MARIA CONCEPCION GARCIA OTADUY
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Radiologia, Hospital das Clínicas, Faculdade de Medicina
LIM/44 - Laboratório de Ressonância Magnética em Neurorradiologia, Hospital das Clínicas, Faculdade de Medicina
LIM/44 - Laboratório de Ressonância Magnética em Neurorradiologia, Hospital das Clínicas, Faculdade de Medicina
12 resultados
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- Exercise modifies hypothalamic connectivity and brain functional networks in women after bariatric surgery: a randomized clinical trial(2023) MEREGE-FILHO, Carlos A. A.; GIL, Saulo S.; KIRWAN, John P.; MURAI, Igor H.; DANTAS, Wagner S.; NUCCI, Mariana P.; PASTORELLO, Bruno; LIMA, Alisson Padilha de; BAZAN, Paulo R.; PEREIRA, Rosa M. R.; SA-PINTO, Ana L. de; LIMA, Fernanda R.; BRUCKI, Sonia M. D.; CLEVA, Roberto de; SANTO, Marco A.; LEITE, Claudia da Costa; OTADUY, Maria Concepcion Garcia; ROSCHEL, Hamilton; GUALANO, BrunoBackgroundObesity is a disease that may involve disrupted connectivity of brain networks. Bariatric surgery is an effective treatment for obesity, and the positive effects on obesity-related conditions may be enhanced by exercise. Herein, we aimed to investigate the possible synergistic effects of Roux-en-Y Gastric Bypass (RYGB) and exercise training on brain functional networks. MethodsThirty women eligible for bariatric surgery were randomly assigned to a Roux-en-Y gastric bypass (RYGB: n = 15, age = 41.0 & PLUSMN; 7.3 years) or RYGB plus Exercise Training (RYGB + ET: n = 15, age = 41.9 & PLUSMN; 7.2 years). Clinical, laboratory, and brain functional connectivity parameters were assessed at baseline, and 3 (POST3) and 9 months (POST9) after surgery. The 6-month, three-times-a-week, exercise intervention (resistance plus aerobic exercise) was initiated 3 months post-surgery (for RYGB + ET). ResultsExercise superimposed on bariatric surgery (RYGB + ET) increased connectivity between hypothalamus and sensorial regions (seed-to-voxel analyses of hypothalamic connectivity), and decreased default mode network (DMN) and posterior salience (pSAL) network connectivity (ROI-to-ROI analyses of brain networks connectivity) when compared to RYGB alone (all p-FDR < 0.05). Increases in basal ganglia (BG) network connectivity were only observed in the exercised training group (within-group analyses). ConclusionExercise training is an important component in the management of post-bariatric patients and may improve the hypothalamic connectivity and brain functional networks that are involved in controlling food intake.
- Gray matter volumes in patients with bipolar disorder and their first-degree relatives(2015) NERY, Fabiano G.; GIGANTE, Alexandre Duarte; AMARAL, Jose A.; FERNANDES, Francy B. F.; BERUTTI, Mariangeles; ALMEIDA, Karla M.; CARNEIROC, Camila de Godoi; DURAN, Fabio Luis Souza; OTADUY, Maria G.; LEITE, Claudia Costa; BUSATTO, Geraldo; LAFER, BenyBipolar disorder (BD) is highly heritable. First-degree relatives of BD patient have an increased risk to develop the disease. We investigated abnormalities in gray matter (GM) volumes in healthy first-degree relatives of BD patients to identify possible brain structural endophenotypes for the disorder. 3D T1-weighted magnetic resonance images were obtained from 25 DSM-IV BD type I patients, 23 unaffected relatives, and 27 healthy controls (HC). A voxel-based morphometry protocol was used to compare differences in GM volumes between groups. BD patients presented reduced GM volumes bilaterally in the thalamus compared with HC. Relatives presented no global or regional GM differences compared with HC. Our negative results do not support the role of GM volume abnormalities as endophenotypes for BD. Thalamic volume abnormalities may be associated the pathophysiology of the disease.
- Quantification of Paramagnetic Ions in Human Brain Tissue Using EPR(2022) OTSUKA, Fabio Seiji; OTADUY, Maria Concepcion Garcia; NASCIMENTO, Otaciro Rangel; SALMON, Carlos Ernesto GarridoThe content and molecular state of paramagnetic ions were assessed by electron paramagnetic resonance (EPR) spectroscopy in ex vivo human brain samples from the globus pallidus, locus coeruleus, and substantia nigra. A dedicated pipeline of quantitative deconvolution and simulation-informed spectral fitting was employed to resolve five overlapping resonance peaks in the EPR signal present in the (whole tissue) brain samples. Simulated results showed good visual agreement with experimental spectral signals. Our quantitative analytical pipeline enabled theorizing understanding of the molecular roles of Iron and other ions in the evaluated specialized subcortical regions. Analysis of the absorption and linewidth of each peak-studied as a function of temperature-showed that four of these peaks behave like a Curie-Weiss paramagnet with an antiferromagnetic contribution, while a broader peak (centered at 320 mT) showed a strong antiferromagnetic behavior with ferro/ferrimagnetic contribution. Noticeably, a fifth peak, which shifts from 190 to 230 mT as temperature increases, was observed exclusively in the locus coeruleus region and can be specifically associated with a possible iron complex formed during the oxidation of dopamine. Our findings support the translational aspects of EPR to inform the cellular-molecular nature of Iron loads phenotypically visible in associated magnetic resonance susceptibility and relaxometry imaging techniques, that are broadly applied to the radiologic characterization of a plethora of neurologic disorders.
- Verbal-memory impairment is associated with widespread white-matter tract involvement in patients with temporal lobe epilepsy and hippocampal sclerosis(2023) OLIVEIRA, Joao Vitor Mahler Ferreira; SOLTI, Marina; ADDA, Carla; JORGE, Carmen; VALERIO, Rosa Maria; LYRA, Katarina; PASTORELLO, Bruno; LEITE, Claudia; OTADUY, Maria; CASTRO, Luiz Henrique Martins
- White matter tract abnormalities are associated with executive dysfunction in temporal lobe epilepsy and hippocampal sclerosis(2023) SOLTI, Marina; OLIVEIRA, Joao Vitor Mahler Ferreira; ADDA, Carla; VALERIO, Rosa; JORGE, Carmen; LYRA, Katarina; PASTORELLO, Bruno; LEITE, Claudia; OTADUY, Maria; CASTRO, Luiz Henrique Martins
conferenceObject ACC neuro-metabolic changes from bipolar depression to euthymia: Repeated 1H-MRS measurement as a function of mood state and lithium efficacy(2020) SOEIRO-DE-SOUZA, M.; SCOTTI-MUZZI, E.; FERNANDES, F.; ZANETTI, M. V.; DESOUZA, R.; LEITE, C.; OTADUY, M. C.; MACHADO-VIEIRA, R.- Cardiac Magnetic Resonance Imaging in a 7 Tesla Magnetic Field: Initial Experience with Hydrogen and Sodium Nuclei(2023) ROCHITTE, Carlos E.; SILVA, Douglas C.; OTADUY, Maria C.; CHAIM, Khallil T.; NOMURA, Cesar H.; CARAMELLI, Bruno
- Global multi-center and multi-modal magnetic resonance imaging study of obsessive-compulsive disorder: Harmonization and monitoring of protocols in healthy volunteers and phantoms(2023) POUWELS, Petra J. W.; VRIEND, Chris; LIU, Feng; JOODE, Niels T. de; OTADUY, Maria C. G.; PASTORELLO, Bruno; ROBERTSON, Frances C.; VENKATASUBRAMANIAN, Ganesan; IPSER, Jonathan; LEE, Seonjoo; BATISTUZZO, Marcelo C.; HOEXTER, Marcelo Q.; LOCHNER, Christine; MIGUEL, Euripedes C.; NARAYANASWAMY, Janardhanan C.; RAO, Rashmi; REDDY, Y. C. Janardhan; SHAVITT, Roseli G.; SHESHACHALA, Karthik; STEIN, Dan J.; BALKOM, Anton J. L. M. van; WALL, Melanie; SIMPSON, Helen Blair; HEUVEL, Odile A. van denObjectives We describe the harmonized MRI acquisition and quality assessment of an ongoing global OCD study, with the aim to translate representative, well-powered neuroimaging findings in neuropsychiatric research to worldwide populations. Methods We report on T1-weighted structural MRI, resting-state functional MRI, and multi-shell diffusion-weighted imaging of 140 healthy participants (28 per site), two traveling controls, and regular phantom scans. Results Human image quality measures (IQMs) and outcome measures showed smaller within-site variation than between-site variation. Outcome measures were less variable than IQMs, especially for the traveling controls. Phantom IQMs were stable regarding geometry, SNR, and mean diffusivity, while fMRI fluctuation was more variable between sites. Conclusions Variation in IQMs persists, even for an a priori harmonized data acquisition protocol, but after pre-processing they have less of an impact on the outcome measures. Continuous monitoring IQMs per site is valuable to detect potential artifacts and outliers. The inclusion of both cases and healthy participants at each site remains mandatory.
- Brain Spectroscopy Analysis in Retired Soccer Players With Chronic Exposure to Mild Traumatic Brain Injuries(2023) RESENDE, Lucas Lopes; LEITE, Claudia da Costa; PASTORELLO, Bruno Fraccini; SOLLA, Davi Jorge Fontoura; MARTINS, Pedro Nascimento; COSTA, Bernardo Fernandes Pelinca da; ARANHA, Mateus Rozalem; FERRACIOLLI, Suely Fazio; OTADUY, Maria Concepcion GarciaSoccer players are at risk of suffering cranial injuries in the short and long term. There is growing concern that this may lead to traumatic brain injury in soccer players. Magnetic resonance spectroscopy (MRS) is an analytical method that enables the measurement of changes in brain metabolites that usually occur before significant structural changes. This study aimed to use MRS to compare variations in brain metabolite levels between retired soccer players and a control group. Twenty retired professional soccer players and 22 controls underwent magnetic resonance imaging, including MRS sequences and Mini-Mental State Examination (MMSE). Metabolite analysis was conducted based on absolute concentration and relative ratios. N-acetyl-aspartate, choline, glutamate, glutamine, and myoinositol were the metabolites of interest for the statistical analysis. Retired soccer players had an average age of 57.8 years, whereas the control group had an average age of 63.2 years. Median cognitive evaluation score, assessed using the MMSE, was 28 [26-29] for athletes and 29 [28-30] for controls (p = 0.01). Uni- and multi-variate analyses of the absolute concentration of metabolites (mM) between former athletes and controls did not yield any statistically significant results. Comparison of metabolites to creatine ratio concentrations did not yield any statistically significant results. There were no changes in concentrations of brain metabolites that indicated brain metabolic changes in retired soccer players compared with controls.
- BDNF rs6265 differentially influences neurometabolites in the anterior cingulate of healthy and bipolar disorder subjects(2023) SCOTTI-MUZZI, Estevao; CHILE, Thais; VALLADA, Homero; OTADUY, Maria Concepcion Garcia; SOEIRO-DE-SOUZA, Marcio GerhardtBrain-derived neurotrophic factor (BDNF) is the most abundant brain neurotrophin and plays a critical role in neuronal growth, survival and plasticity, implicated in the pathophysiology of Bipolar Disorders (BD). The single-nucleotide polymorphism in the BDNF gene (BDNF rs6265) has been associated with decreased hippocampal BDNF secretion and volume in met carriers in different populations, although the val allele has been reported to be more frequent in BD patients. The anterior cingulate cortex (ACC) is a key center integrating cognitive and affective neuronal connections, where consistent alterations in brain metabolites such as Glx (Glutamate + Glutamine) and N-acetylaspartate (NAA) have been consistently reported in BD. However, little is known about the influence of BDNF rs6265 on neurochemical profile in the ACC of Healthy Controls (HC) and BD subjects. The aim of this study was to assess the influence of BDNF rs6265 on ACC neurometabolites (Glx, NAA and total creatine- Cr) in 124 euthymic BD type I patients and 76 HC, who were genotyped for BDNF rs6265 and underwent a 3-Tesla proton magnetic resonance imaging and spectroscopy scan ((1) H-MRS) using a PRESS ACC single-voxel (8cm(3)) sequence. BDNF rs6265 polymorphism showed a significant two-way interaction (diagnosis x genotype) in relation to NAA/Cr and total Cr. While met carriers presented increased NAA/Cr in HC, BD-I subjects with the val allele revealed higher total Cr, denoting an enhanced ACC metabolism likely associated with increased glutamatergic metabolites observed in BD-I val carriers. However, these results were replicated only in men. Therefore, our results support evidences that the BDNF rs6265 polymorphism exerts a complex pleiotropic effect on ACC metabolites influenced by the diagnosis and sex.