SOLANGE CARRASCO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- EARLY SACROILIITIS AND PROGRESSION TO ANKYLOSING SPONDYLITIS IS ASSOCIATED TO POSITIVE HLA-B27 IN JUVENILE SPONDYLOARTHRITIS(2015) PEREZ, M. O.; AIKAWA, N. E.; CARRASCO, S.; SAAD, C. G.; SAMPAIO-BARROS, P. D.; GONCALVES, C. R.; MORAES, J. C. B.; GOLDENSTEIN-SCHAINBERG, C.
conferenceObject HLA-B27 PREVALENCE IN A COHORT OF BRAZILIAN PATIENTS WITH PSORIATIC ARTHRITIS AND ANKYLOSING SPONDYLITIS(2014) GOLDENSTEIN-SCHAINBERG, C.; CARRASCO, S.; SAAD, C. G.; MORAES, J. C. B.; GONCALVES, C. R.; SAMPAIO-BARROS, P.; PARRA, E. R.conferenceObject PREVALENCE AND CHARACTERIZATION OF PSORIATIC ARTHRITIS MUTILANS PATIENTS FROM A SINGLE TERTIARY CENTER(2018) OLIVEIRA, J. L.; CORDEIRO, R. A.; CARRASCO, S.; GONCALVES, C. R.; MORAES, J. C. B.; SAAD, C. G. S.; SAMPAIO-BARROS, P. D.; GOLDENSTEIN-SCHAINBERG, C.conferenceObject HLA-B27 ANALYSIS BY FLOW CITOMETRY IN BRAZILIAN PATIENTS WITH ANKYLOSING SPONDYLITIS AND PSORIATIC ARTHRITIS(2013) GOLDENSTEIN-SCHAINBERG, C.; CARRASCO, S.; SAAD, C.; GONCALVES, C.; SAMPAIO-BARROS, P.; PARRA, E.conferenceObject Positive HLA-B27 in Juvenile Spondyloarthritis Is Associated to Early Sacroiliitis and Progression to Ankylosing Spondylitis(2014) PEREZ, Mariana O.; AIKAWA, Nadia E.; CARRASCO, Solange; SAMPAIO-BARROS, Percival D.; GONCALVES, Celio R.; SAAD, Carla G. S.; MORAES, Julio C. B.; GOLDEINSTEIN-SCHAINBERG, ClaudiaconferenceObject INCREASED TOLL LIKE RECEPTOR 2 (TLR2) EXPRESSION ON PERIPHERAL BLOOD MONOCYTES FROM PATIENTS WITH ANTI-TNF INDUCED PSORIASIS SUGGESTS A ROLE FOR A GRAM-POSITIVE INFLAMMATORY TRIGGER(2016) VALKINIR, D. E. J.; FERNANDEZ, V. V.; NOGUEIRA, M. A. D. S.; ROMITI, R.; AMONE, M.; HIRAYAMA, A. L. D. S.; TAKAHASHI, M. D. F.; CARRASCO, S.; SAMPAIO-BARROS, P.; MORAES, J.; GONCALVES, C.; SAAD, C.; GOLDENSTEIN-SCHAINBERG, C.article Toll-like receptor (TLR) 2 is upregulated on peripheral blood monocytes of patients with psoriatic arthritis: a role for a gram-positive inflammatory trigger?(2011) CARRASCO, S.; NEVES, F. S.; FONSECA, M. H.; GONCALVES, C. R.; SAAD, C. G.; SAMPAIO-BARROS, P. D.; GOLDENSTEIN-SCHAINBERG, C.Toll-like receptor (TLR) 2 and TLR4 are able to activate innate immune cells in response to gram-positive and gram-negative bacteria, respectively. Psoriatic arthritis (PsA) is a chronic inflammatory joint disease and gram-positive streptococcus may have a role in its pathogenesis, suggesting the importance of TLR2 stimulation in PsA. Objective To assess TLR2 and TLR4 expressions on innate immune cells of PsA patients, relating to clinical disease activity. Methods Fort-five patients with peripheral joint manifestations of PsA were included and disease activity was assessed by Disease Activity Score of 28 joint counts (DAS28). 32 healthy subjects constituted the control group. Membrane-bound TLR2 and TLR4 expressions were assessed on peripheral blood monocytes and neutrophils by flow cytometry. Results Twenty-seven patients had active PsA (DAS28 higher than 2.6) and 18 had inactive disease. TLR2 was significantly upregulated on monocytes in both active and inactive PsA group, comparing to healthy controls. TLR4 was similarly expressed in all tested groups. Conclusion TLR2 is overexpressed by PsA monocytes, suggesting that gram-positive exposure could induce higher inflammatory responses in this disease.- Circulating Follicular Helper-Like T Cells in Systemic Lupus Erythematosus(2015) CHOI, Jin-Young; HO, John Hsi-en; PASOTO, Sandra G.; BUNIN, Viviane; KIM, Sang Taek; CARRASCO, Solange; BORBA, Eduardo F.; GONCALVES, Celio R.; COSTA, Priscila R.; KALLAS, Esper G.; BONFA, Eloisa; CRAFT, JosephObjective. To assess circulating follicular helper T (Tfh)-like CD4+ T cells in patients with systemic lupus erythematosus (SLE) and determine their relationship to disease activity. Methods. Blood samples from patients with SLE, as well as blood samples from patients with Behcet's disease (BD) and healthy individuals as controls, were analyzed. In all samples, circulating Tfh-like cells were enumerated by flow cytometry, using, as markers, expression of CXCR5, inducible T cell costimulator (ICOS), and programmed death 1 (PD-1) protein, as well as secretion of interleukin-21 (IL-21). The frequency of circulating Tfh-like cells was compared to that of circulating plasmablasts (CD19+IgD-CD38+). In addition, the possible association of circulating Tfh-like cells with the SLE Disease Activity Index (SLEDAI) was evaluated. Results. The subset of circulating Tfh-like T cells, identified as CXCR5(high)ICOS(high)PD-1(high), was expanded in the blood of SLE patients compared to controls. Circulating Tfh-like cells were found to produce IL-21 and had lower expression of CCR7 as compared to that in circulating CXCR5(high) central memory T cells, thereby enabling their distinction. Expression of PD-1, but not ICOS or CXCR5, was significantly elevated in circulating Tfh-like cells from SLE patients compared to controls. PD-1 expression among CXCR5(high) circulating Tfh-like cells correlated with the SLEDAI, frequency of circulating plasmablasts, and anti-double-stranded DNA antibody positivity, but not with disease duration or past organ injury; rather, this cell profile appeared to be a reflection of current active disease. Conclusion. Circulating Tfh-like cells are associated with disease activity in SLE, suggesting that their presence indicates abnormal homeostasis of T cell-B cell collaboration, with a causal relationship that is central to disease pathogenesis. These findings also suggest that circulating Tfh-like cells provide a surrogate for aberrant germinal center activity in SLE, and that their PD-1 expression offers a tool for measuring disease activity and monitoring the response to therapies.
conferenceObject OVEREXPRESSION OF TOLL-LIKE RECEPTOR 2 ON PERIPHERAL BLOOD MONOCYTES FROM PATIENTS WITH PSORIATIC ARTHRITIS (PsA)(2012) CARRASCO, S.; NEVES, F. S.; GAMBIM, M.; SAAD, C. G.; GONCALVES, C.; SAMPAIO-BARROS, P.; GOLDENSTEIN-SCHAINBERG, C.Toll-like receptor (TLR) 2 and TLR4 are able to activate innate immune cells in response to gram-positive and gramnegative bacteria, respectively. Psoriatic arthritis (PsA) is a chronic inflammatory joint disease and gram-positive streptococcus may have a role in its pathogenesis, suggesting the importance of TLR2 stimulation in PsA. OBJECTIVES: To assess TLR2 and TLR4 expressions on innate immune cells of PsA patients, relating to clinical disease activity. METHODS: Forty-five patients with peripheral joint manifestations of PsA were included and disease activity was assessed by Disease Activity Score of 28 joint counts (DAS28). 32 healthy subjects constituted the control group. Membrane-bound TLR2 and TLR4 expressions were assessed on peripheral blood monocytes and neutrophils by flow cytometry. RESULTS: Twenty-seven patients had active PsA (DAS28 higher than 2.6) and 18 had inactive disease. TLR2 was significantly upregulated on monocytes in both active and inactive PsA group, comparing to healthy controls. TLR4 was similarly expressed in all tested groups. CONCLUSIONS: TLR2 is overexpressed by PsA monocytes, suggesting that gram-positive exposure could induce higher inflammatory responses in this disease.conferenceObject JUVENILE SPONDYLOARTHRITIS (JSpA) IN A COHORT OF BRAZILIAN PATIENTS(2014) PEREZ, M. O.; AIKAWA, N. E.; CARRASCO, S.; SAMPAIO-BARROS, P. D.; GONCALVES, C. R.; SAAD, C. G. S.; MORAES, J. C. B.; GOLDENSTEIN-SCHAINBERG, C.