SOLANGE CARRASCO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Ultra High Dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro(2011) GUEDES, J. R. P.; CARRASCO, S.; FERREIRA, C. M.; BONAMIN, L. V.; SOUZA, W.; GOLDENSTEIN-SCHAINBERG, C.; PARRA, E. R.; CAPELOZZI, V. L.Background: Ultra High Dilutions (UHD) are diluted beyond the Avogadro limit with dynamization (dilution with succussion). The process of anuran amphibian metamorphosis is controlled by thyroid hormones, including the resorption of the tadpole tail. Methods: A randomized and blinded study was performed to investigate the influence of triiodothyronine (T3) 5.10(-24) M (10cH) on apoptosis induced by T3 100 nM in Rana catesbeiana tadpoles' tail tips, in vitro. Explants were randomized to three groups: control: no T3 in pharmacological or UHD dose; test: T3 100 nM and challenged with T3 10cH (UHD); positive control: T3 100 nM, treated with unsuccussed ethanol. The apoptotic index and the area of explants of test and control groups at the first and final day of the experiment were compared by t-test. Results: There was no difference in tail tip area between test and control groups, but a significantly higher (p < 0.01) index of apoptosis in explants of the test group. Conclusion: This data suggest that T3 10cH modifies the effect of T3 at pharmacological dose, opening new perspectives for further studies and investigation of the dose effect curve. Homeopathy (2011) 100, 220-227.
article Toll-like receptor (TLR) 2 is upregulated on peripheral blood monocytes of patients with psoriatic arthritis: a role for a gram-positive inflammatory trigger?(2011) CARRASCO, S.; NEVES, F. S.; FONSECA, M. H.; GONCALVES, C. R.; SAAD, C. G.; SAMPAIO-BARROS, P. D.; GOLDENSTEIN-SCHAINBERG, C.Toll-like receptor (TLR) 2 and TLR4 are able to activate innate immune cells in response to gram-positive and gram-negative bacteria, respectively. Psoriatic arthritis (PsA) is a chronic inflammatory joint disease and gram-positive streptococcus may have a role in its pathogenesis, suggesting the importance of TLR2 stimulation in PsA. Objective To assess TLR2 and TLR4 expressions on innate immune cells of PsA patients, relating to clinical disease activity. Methods Fort-five patients with peripheral joint manifestations of PsA were included and disease activity was assessed by Disease Activity Score of 28 joint counts (DAS28). 32 healthy subjects constituted the control group. Membrane-bound TLR2 and TLR4 expressions were assessed on peripheral blood monocytes and neutrophils by flow cytometry. Results Twenty-seven patients had active PsA (DAS28 higher than 2.6) and 18 had inactive disease. TLR2 was significantly upregulated on monocytes in both active and inactive PsA group, comparing to healthy controls. TLR4 was similarly expressed in all tested groups. Conclusion TLR2 is overexpressed by PsA monocytes, suggesting that gram-positive exposure could induce higher inflammatory responses in this disease.