MARINA FARREL CORTES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Evaluation of loop-mediated isothermal amplification assay for detection of Clostridioides difficile infection: A prospective diagnostic performance study(2021) GIRA, Evelyne Santana; NOGUERA, Saidy Liceth Vasconez; ESPINOZA, Evelyn Patricia Sanchez; BEZERRA, Camila Loredana Pereira Alves Madeira; LINO, Fernando Molina; CORTES, Marina Farrel; SANTOS, Sania Alves dos; PARANHOS-BACCALA, Glaucia; PERDIGA, Lauro Vieira; COSTA, Silvia FigueiredoA novel Loop-mediated isothermal amplification (LAMP) assay, HiberGene's CD was evaluated with 82 unformed stools from patients suspected of C. difficile infection (CDI). Compared to glutamate dehy-drogenase (GDH) toxins A/B test (C.diff Quik Chek (R)), HiberGene's LAMP showed 100% of sensitivity and 95,8% of specificity; and compared to FilmArrayTM GI panel (R) (BioFire), a sensitivity of 81,2% and a specificity of 100%, with 96.38% of agreement.
- Are mobile phones part of the chain of transmission of SARS- CoV-2 in hospital settings?(2021) ESPINOZA, Evelyn Patricia Sanchez; CORTES, Marina Farrel; NOGUERA, Saidy Vasconez; PAULA, Anderson Vicente de; GUIMARAES, Thais; BOAS, Lucy Santos Villas; PARK, Marcelo; SILVA, Cristina Carvalho da; MORALES, Ingra; NETO, Lauro Vieira Perdigao; TOZETTO-MENDOZA, Tania Regina; BOSZCZOWSKI, Icaro; SABINO, Ester Cerdeira; MENDES-CORREA, Maria Cassia; LEVIN, Anna Sara; COSTA, Silvia FigueiredoMobile phones (MPs) have become an important work tool around the world including in hospitals. We evaluated whether SARS-CoV-2 can remain on the surface of MPs of first-line healthcare workers (HCW) and also the knowledge of HCWs about SARS-CoV-2 cross-transmission and conceptions on the virus survival on the MPs of HCWs. A crosssectional study was conducted in the COVID-19 Intensive Care Unit of a teaching hospital. An educational campaign was carried out on cross-transmission of SARS-CoV-2, and its permanence in fomites, in addition to the proper use and disinfection of MPs. Herewith an electronic questionnaire was applied including queried conceptions about hand hygiene and care with MP before and after the pandemic. The MPs were swabbed with a nylon FLOQ SwabTM, in an attempt to increase the recovery of SARS-CoV-2. All MP swab samples were subjected to SARS-CoV-2 RT-PCR; RT-PCR positive samples were subjected to viral culture in Vero cells (ATCC (R) CCL-81TM). Fifty-one MPs were swabbed and a questionnaire on hand hygiene and the use and disinfection of MP was applied after an educational campaign. Most HCWs increased adherence to hand hygiene and MP disinfection during the pandemic. Fifty-one MP swabs were collected and two were positive by RT-PCR (4%), with Cycle threshold (Ct ) values of 34-36, however, the cultures of these samples were negative. Although most HCWs believed in the importance of cross-transmission and increased adherence to hand hygiene and disinfection of MP during the pandemic, SARS-CoV-2 RNA was detected in MPs. Our results suggest the need for a universal policy in infection control guidelines on how to care for electronic devices in hospital settings.
- Disinfection of 3D-printed protective face shield during COVID-19 pandemic(2021) NOGUERA, Saidy Vasconez; ESPINOZA, Evelyn Patricia Sanchez; CORTES, Marina Farrel; OSHIRO, Izabel Cristina Vilela; SPADAO, Fernanda de Sousa; BRANDAO, Laura Maria Brasileiro; BARROS, Ana Natiele da Silva; COSTA, Sibeli; ALMEIDA, Bianca Leal de; SORIANO, Paula Gemignani; SALLES, Alessandra Grassi; ESCORCIO, Mirian Elizabete Marques; BARRETTI, Cristina Madeira; BAPTISTA, Fernanda Spadotto; ALVARENGA, Glaura Souza; MARINHO, Igor; LETAIF, Leila Suemi Harima; LI, Ho Ye; BACCHI, Pedro; SANTOS, Ana Rubia Guedes dos; REGADAS, Lucas Borges; BRAGA, Carlos Eduardo Lima; ZSIGMOND, Fabio; SEGURADO, Aluisio Cotrim; GUIMARAES, Thais; LEVIN, Anna Sara; BERTOLDI, Cristiane Aun; CATALANI, Luiz Henrique; ZANCUL, Eduardo de Senzi; COSTA, Silvia FigueiredoThis study assessed the disinfection using 70% ethanol; H2O2-quaternary ammonium salt mixture; 0.1% sodium hypochlorite and autoclaving of four 3D-printed face shields with different designs, visor materials; and visor thickness (0.5-0.75 mm). We also investigated their clinical suitability by applying a questionnaire to health workers (HW) who used them. Each type of disinfection was done 40 times on each type of mask without physical damage. In contrast, autoclaving led to appreciable damage.
- Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections(2021) PAIVA, Yrving; NAGANO, Debora Satie; COTIA, Andre Luis Franco; GUIMARAES, Thais; MARTINS, Roberta Cristina Ruedas; PERDIGAO NETO, Lauro Vieira; CORTES, Marina Farrel; MARCHI, Ana Paula; CORSCADDEN, Louise; MACHADO, Anna Silva; PAULA, Alexandre Inacio de; FRANCO, Lucas Augusto Moyses; NEVES, Patricia Regina; LEVIN, Anna Sara; COSTA, Silvia FigueiredoThe plasmid-mediated colistin-resistance gene named mcr-1 has been recently described in different countries and it became a public health challenge. Of note, few studies have addressed the spread of Escherichia coli harboring the mcr-1 gene in both, community and hospital settings. A total of seven colistin-resistant E. coli carrying mcr-1, collected from 2016 to 2018, from community (n=4), healthcare-acquired infections (n=2) and colonization (n=1) were identified in three high complexity hospitals in Sao Paulo, Brazil. These colistin-resistant isolates were screened for mcr genes by PCR and all strains were submitted to Whole Genome Sequencing and the conjugation experiment. The seven strains belonged to seven distinct sequence types (ST744, ST131, ST69, ST48, ST354, ST57, ST10), and they differ regarding the resistance profiles. Transference of mcr-1 by conjugation to E. coli strain C600 was possible in five of the seven isolates. The mcr-1 gene was found in plasmid types IncX4 or IncI2. Three of the isolates have ESBL-encoding genes (bla(CTX-M-2), n=2; bla(CTX-M-8), n=1). We hereby report genetically distinct E. coli isolates, belonging to seven STs, harboring the mcr-1 gene, associated to community and healthcare-acquired infections, and colonization in patients from three hospitals in Sao Paulo. These findings point out for the potential spread of plasmid-mediated colistin-resistance mechanism in E. coli strains in Brazil.
- Carbapenem-resistant Klebsiella pneumoniae colonization and infection is associated with lower overall survival in a cohort of haematopoietic stem-cell transplantation patients: mechanism of resistance and virulence by whole-genome sequencing(2021) HIGASHINO, Hermes Ryoiti; MARCHI, Ana Paula; MARTINS, Roberta Cristina Ruedas; CARVALHO, Laina Bubach; PERDIGAO NETO, Lauro Vieira; CORTES, Marina Farrel; OLIVEIRA, Fernando Nivaldo de; DUARTE, Edson Luiz Tarsia; GUIMARAES, Thais; ROSSI, Flavia; FERREIRA, Aliana M.; ROCHA, Vanderson; COSTA, Silvia FigueiredoCarbapenem-resistant Klebsiella pneumoniae (CRK) infections are a growing concern in immunocompromised patients. The aim of the present study was to evaluate the impact of CRK colonization and infection in overall mortality for haematopoietic stem-cell transplant (HSCT) patients. We also aimed to investigate resistance and virulence profiles of CRK isolates and assess their epidemiological and genetic relatedness. Patients in the HSCT unit were screened for colonization with CRK with weekly rectal swab or stool cultures and placed under contact precautions. We defined CRK colonization as positive culture from a swab or stool sample grown in MacConkey agar with meropenem at 1 mu g ml(-1). Demographic and clinical data were retrieved from the patients' charts and electronic records. According to resistance mechanisms and pulsed field gel electrophoresis profile, isolates were selected based on whole-genome sequencing (WGS) using MiSeq Illumina. Outcomes were defined as overall mortality (death up to D+100), and infection-related death (within 14 days of infection). We report a retrospective cohort of 569 haematopoietic stem-cell transplant patients with 105 (18.4 %) CRK colonizations and 30 (5.3 %) infections. blaKPC was the most frequent carbapenemase in our cohort with three isolates co-harbouring blaKPC and blaNDM. We found no difference in virulence profiles from the CRK isolates. There were also no significant differences in virulence profiles among colonization and infection isolates regarding genes encoding for type 1 and 3 fimbriae, siderophores, lipopolysaccharide and colibactin. In clonality analysis by PFGE and WGS, isolates were polyclonal and ST340 was the most prevalent. Overall survival at D+100 was 75.4% in in CRK-colonized (P=0.02) and 35.7 % in infected patients and significantly lower than non-colonized patients (85.8 %; P<0.001). We found a higher overall mortality associated with colonization and infection; KPC was the main resistance mechanism for carbapenems. The polyclonal distribution of isolates and findings of CRK infection in patients not previously colonized suggest the need to reinforce antibiotic stewardship.
- Procalcitonin as a biomarker for ventilator associated pneumonia in COVID-19 patients: Is it an useful stewardship tool?(2021) CORTES, Marina Farrel; ALMEIDA, Bianca Leal de; ESPINOZA, Evelyn Patricia Sanchez; CAMPOS, Aleia Faustina; MOURA, Maria Luiza do Nascimento; SALOMAO, Matias C.; BOSZCZOWSKI, Icaro; FREIRE, Maristela Pinheiro; CARVALHO, Laina Bubach de; PARANHOS-BACCALA, Glaucia; COSTA, Silvia Figueiredo; GUIMARAES, ThaisVentilator associated pneumonia(VAP) is a severe complication that can lead to high mortality when not early identified or when therapy is delayed. The aim of this study was to evaluate procalcitonin(PCT) as a biomarker for VAP development. In total, 73 hospitalized patients with COVID-19 were analyzed. PCT levels greater than 0.975ng/mL were more related to VAP. No association was found for C-reactive protein (CRP). The results show that procalcitonin may be a pertinent biomarker for VAP diagnosis and can be a helpful tool for antibiotic withdrawal.
- Decontamination and re-use of surgical masks and respirators during the COVID-19 pandemic(2021) CORTES, Marina Farrel; ESPINOZA, Evelyn Patricia Sanchez; NOGUERA, Saidy Liceth Vasconez; SILVA, Aline Alves; MEDEIROS, Marion Elke Sielfeld Araya de; BOAS, Lucy Santos Villas; FERREIRA, Noely Evangelista; TOZETTO-MENDOZA, Tania Regina; MORAIS, Fernando Goncalves; QUEIROZ, Rayana Santiago de; PROENCA, Adriana Coracini Tonacio de; GUIMARAES, Thais; GUEDES, Ana Rubia; LETAIF, Leila Suemi Harima; MONTAL, Amanda Cardoso; MENDES-CORREA, Maria Cassia; JOHN, Vanderley M.; LEVIN, Anna S.; COSTA, Silvia FigueiredoObjectives: The coronavirus disease 2019 pandemic increased global demand for personal protective equipment (PPE) and resulted in shortages. The study evaluated the re-use of surgical masks and respirators by analysing their performance and safety before and after reprocessing using the following methods: oven, thermal drying, autoclave, and hydrogen peroxide plasma vapour. Methods: In total, 45 surgical masks and 69 respirators were decontaminated. Visual integrity, air permeability, burst resistance, pressure differential and particulate filtration efficiency of new and decontaminated surgical masks and respirators were evaluated. In addition, 14 used respirators were analysed after work shifts before and after decontamination using reverse transcription polymerase chain reaction (RT-PCR) and viral culturing. Finally, reprocessed respirators were evaluated by users in terms of functionality and comfort. Results: Oven decontamination (75 degrees C for 45 min) was found to be the simplest decontamination method. Physical and filtration assays indicated that all reprocessing methods were safe after one cycle. Oven decontamination maintained the characteristics of surgical masks and respirators for at least five reprocessing cycles. Viral RNA was detected by RT-PCR in two of the 14 used respirators. Four respirators submitted to viral culture were PCR-negative and culture-negative. Reprocessed respirators used in work shifts were evaluated positively by users, even after three decontamination cycles. Conclusion: Oven decontamination is a safe method for reprocessing surgical masks and respirators for at least five cycles, and is feasible in the hospital setting. (C) 2020 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.