FERNANDA DEGOBBI TENORIO QUIRINO DOS SANTOS LOPES
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
15 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 15
- The tick-derived rBmTI-A protease inhibitor attenuates the histological and functional changes induced by cigarette smoke exposure(2018) LOURENCO, Juliana D.; ITO, Juliana T.; CERVILHA, Daniela A. B.; SALES, Davi S.; RIANI, Alyne; SUEHIRO, Camila L.; GENARO, Isabella S.; DURAN, Adriana; PUZER, Luciano; MARTINS, Milton A.; SASAKI, Sergio D.; LOPES, Fernanda D. T. Q. S.Introduction. Smoking is the main risk factor for chronic obstructive pulmonary disease development and cigarette smoke (CS) exposure is considered an important approach to reproduce in rodents this human disease. We have previously shown that in an elastase induced model of emphysema, the administration of a protease inhibitor (rBmTI-A) prevented and attenuated tissue destruction in mice. Thus, in this study we aimed to verify the effects of rBmTI-A administration on the physiopathological mechanisms of CS induced emphysema. Methods. Mice (C57BL/6) were exposed to CS or room air for 12 weeks. In this period, 3 nasal instillations of rBmTI-A inhibitor or its vehicle were performed. After euthanasia, respiratory mechanics were evaluated and lungs removed for analysis of mean linear intercept, volume proportion of collagen and elastic fibers, density of polymorphonuclear cells, macrophages, and density of positive cells for MMP-12, MMP-9, TIMP-1 and gp91phox. Results. The rBmTI-A administration improved tissue elastance, decreased alveolar enlargement and collagen fibers accumulation to control levels and attenuated elastic fibers accumulation in animals exposed to CS. There was an increase of MMP-12, MMP-9 and macrophages in CS groups and the rBmTIA only decreased the number of MMP-12 positive cells. Also, we demonstrated an increase in gp91phox in CS treated group and in TIMP-1 levels in both rBmTI-A treated groups. Conclusion. In summary, the rBmTI-A administration attenuated emphysema development by an increase of gp91phox and TIMP-1, accompanied by a decrease in MMP-12 levels.
conferenceObject Metalloproteases gene expression and remodeling of lung parenchyma fibers during the progression of elastase induced emphysema(2014) ROBERTONI, Fabiola Santos Zambon; OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; GONCALVES, Natalia Comes; VELOSA, Ana Paula Pereira; TEODORO, Walcy Rosolia; LIN, Chin Jia; MARTINS, Milton De Arruda; LOPES, Fernanda D. T. Q. dos Santos- The role of monoterpenes derived from essential oils in lung alteration in a model of emphysema(2016) PEREIRA, Ellen; GUERREIRO, Marina; SANTANA, Fernanda; PINHEIRO, Nathalia; CAPELLO, Tabata; LOPES, Fernanda; OLIVO, Clarice; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, Carla
conferenceObject Temporal profile of metaloproteases gene expression in elastase-induced emphysema(2013) ROBERTONI, Fabiola Santos Zambon; OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; GANCALVES, Natalia Gomes; VELOSA, Ana Paula Pereira; TEODORO, Walcy Rosolia; LIN, Chin Jia; MARTINS, Milton de Arruda; LOPES, Fernanda Degobbi Tenorio Q. S.- Temporal analysis of the intracellular signaling pathways involved in Th17/Treg response in COPD development(2019) SILVA, Larissa Emidio de Franca; LOURENCO, Juliana Dias; SILVA, Kaique Rodrigues Da; KOHLER, Julia Benini; SANTANA, Fernanda Paula Roncon; MOREIRA, Alyne Riani; CERVILHA, Daniela Aparecida De Brito; HAMAGUCHI, Sara Sumie Sobral; PRADO, Carla Maximo; VIEIRA, Rodolfo De Paula; VELOSA, Ana Paula Pereira; ITO, Juliana Tiyaki; LOPES, Fernanda Degobbi Tenorio Quirino Dos Santos
conferenceObject Histological analysis of compensatory lung growth effects in emphysematous rats after bilobectomy(2013) ALMEIDA, Francine Maria; SARAIVA-ROMANHOLO, Beatriz M.; PAULA, Rodolfo de; MORIYA, Henrique Takachi; LIGEIRO-DE-OLIVEIRA, Ana Paula; LOPES, Fernanda D. Q. S.; MAUAD, Thais; MARTINS, Milton de Arruda; PAZETTI, RogerioconferenceObject Time course effects of exercise training on pulmonary injury induced by exposure to cigarette smoke in mice(2013) TOLEDO-ARRUDA, Alessandra C.; GUARNIER, Flavia; SUEHIRO, Camila L.; ALMEIDA, Francine; OLIVO, Clarice; LOPES, Fernanda; VIEIRA, Rodolfo; CAMARGO-FILHO, Jose Carlos Silva; CECCHINI, Rubens; LIN, Chin; MARTINS, Milton A.- Low dose of sodium hypochlorite impair lung function, inflammation and oxidative stress of naive mice(2019) GENARO, Isabella Santos De; SILVA, Stefani Cristina Da; NANNI, Gabriela Delourence; ARAUJO, Lethicia Cristinne Silva; MEDEIROS, Rebeca Roberta Campos; LIEVORE, Giovanna Del Vecchi; ALMEIDA, Francine Maria De; BEZERRA, Suellen Karoline Moreira; KUNZLER, Deborah De Camargo Hizume; LOPES, Fernanda Degobbi Tenorio Quirino Dos Santos; VIEIRA, Rodolfo De Paula; MARTINS, Milton De Arruda; TIBERIO, Iolanda De Fatima Lopes Calvo; SARAIVA-ROMANHOLO, Beatriz Mangueira
- Different Phenotypes in Asthma: Clinical Findings and Experimental Animal Models(2022) LOURENCO, Luiz Otavio; RIBEIRO, Alessandra Mussi; LOPES, Fernanda Degobbi Tenorio Quirino Dos Santos; TIBERIO, Iolanda de Fatima Lopes Calvo; TAVARES-DE-LIMA, Wothan; PRADO, Carla MaximoAsthma is a respiratory allergic disease presenting a high prevalence worldwide, and it is responsible for several complications throughout life, including death. Fortunately, asthma is no longer recognized as a unique manifestation but as a very heterogenic manifestation. Its phenotypes and endotypes are known, respectively, as pathologic and molecular features that might not be directly associated with each other. The increasing number of studies covering this issue has brought significant insights and knowledge that are constantly expanding. In this review, we intended to summarize this new information obtained from clinical studies, which not only allowed for the creation of patient clusters by means of personalized medicine and a deeper molecular evaluation, but also created a connection with data obtained from experimental models, especially murine models. We gathered information regarding sensitization and trigger and emphasizing the most relevant phenotypes and endotypes, such as Th2-(high) asthma and Th2-(low) asthma, which included smoking and obesity-related asthma and mixed and paucigranulocytic asthma, not only in physiopathology and the clinic but also in how these phenotypes can be determined with relative similarity using murine models. We also further investigated how clinical studies have been treating patients using newly developed drugs focusing on specific biomarkers that are more relevant according to the patient's clinical manifestation of the disease.
conferenceObject A protease inhibitor from the tick rhipicephalus boophilus microplus attenuated parenchymal destruction in elastase-induced emphysema(2013) OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; ALMEIDA, Francine M.; NEVES, Luana Paiva; ARANTES, Petra Mello; PRADO, Carla Maximo; TIBERIO, Iolanda; TANAKA, Aparecida S.; SASAKI, Sergio D.; MARTINS, Milton Arruda; LOPES, Fernanda D. T. Q. S.