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LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
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- Multiplex qPCR Discriminates Variants of Concern to Enhance Global Surveillance of SARS-CoV-2(2023) VOGELS, C. B. F.; BREBAN, M. I.; OTT, I. M.; ALPERT, T.; PETRONE, M. E.; WATKINS, A. E.; KALINICH, C. C.; EARNEST, R.; ROTHMAN, J. E.; JESUS, J. G. de; CLARO, I. M.; FERREIR, G. M.; CRISPIM, M. A. E.; SINGH, L.; TEGALLY, H.; ANYANEJI, U. J.; HODCROF, E. B.; MASON, C. E.; KHULLAR, G.; METTI, J.; DUDLEY, J. T.; MACKAY, M. J.; NASH, M.; WANG, J.; LIU, C.; HUI, P.; MURPHY, S.; NEAL, C.; LASZLO, E.; LANDRY, M. L.; MUYOMBWE, A.; DOWNING, R.; RAZEQ, J.; OLIVEIRA, T. de; FARIA, N. R.; SABINO, E. C.; NEHER, R. A.; FAUVER, J. R.; GRUBAUGH, N. D.Broadly accessible and inexpensive surveillance methods are needed to track Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOC) around the world. While sequencing is the gold standard to identify circulating SARS-CoV-2 variants, routine genomic surveillance is not available in many locations primarily due to a lack of resources and expertise. The Institutional Review Board from the Yale University Human Research Protection Program determined that the RT-qPCR testing and sequencing of de-identified remnant COVID-19 clinical samples conducted in this study is not research involving human patients. Multiplex-PCR products were purified by using AmpureXP beads, and quantification was carried out using the Qubit dsDNA High Sensitivity assay on the Qubit 3.0. © 2023 Jenny Stanford Publishing Pte. Ltd.