MARIA LUCIA HIRATA KATAYAMA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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- Somatic mutations in breast and serous ovarian cancer young patients: a systematic review and meta-analysis(2015) ENCINAS, Giselly; MAISTRO, Simone; PASINI, Fatima Solange; KATAYAMA, Maria Lucia Hirata; BRENTANI, Maria Mitzi; BOCK, Geertruida Hendrika de; FOLGUEIRA, Maria Aparecida Azevedo KoikeObjective: our aim was to evaluate whether somatic mutations in five genes were associated with an early age at presentation of breast cancer (BC) or serous ovarian cancer (SOC). Methods: COSMIC database was searched for the five most frequent somatic mutations in BC and SOC. A systematic review of PubMed was performed. Young age for BC and SOC patients was set at <= 35 and <= 40 years, respectively. Age groups were also classified in <30years and every 10 years thereafter. Results: twenty six (1,980 patients, 111 younger) and 16 studies (598, 41 younger), were analyzed for BC and SOC, respectively. In BC, PIK3CA wild type tumor was associated with early onset, not confirmed in binary regression with estrogen receptor (ER) status. In HER2-negative tumors, there was increased frequency of PIK3CA somatic mutation in older age groups; in ER-positive tumors, there was a trend towards an increased frequency of PIK3CA somatic mutation in older age groups. TP53 somatic mutation was described in 20% of tumors from both younger and older patients; PTEN, CDH1 and GATA3 somatic mutation was investigated only in 16 patients and PTEN mutation was detected in one of them. In SOC, TP53 somatic mutation was rather common, detected in more than 50% of tumors, however, more frequently in older patients. Conclusion: frequency of somatic mutations in specific genes was not associated with early-onset breast cancer. Although very common in patients with serous ovarian cancer diagnosed at all ages, TP53 mutation was more frequently detected in older women.
- Specific upregulation of RHOA and RAC1 in cancer-associated fibroblasts found at primary tumor and lymph node metastatic sites in breast cancer(2015) ROZENCHAN, Patricia Bortman; PASINI, Fatima Solange; ROELA, Rosimeire A.; KATAYAMA, Maria Lucia Hirata; MUNDIM, Fiorita Gonzales Lopes; BRENTANI, Helena; LYRA, Eduardo C.; BRENTANI, Maria MitziThe importance of tumor-stromal cell interactions in breast tumor progression and invasion is well established. Here, an evaluation of differential genomic profiles of carcinoma-associated fibroblasts (CAFs) compared to fibroblasts derived from tissues adjacent to fibroadenomas (NAFs) revealed altered focal adhesion pathways. These data were validated through confocal assays. To verify the possible role of fibroblasts in lymph node invasion, we constructed a tissue microarray consisting of primary breast cancer samples and corresponding lymph node metastasis and compared the expression of adhesion markers RhoA and Rac1 in fibroblasts located at these different locations. Two distinct tissue microarrays were constructed from the stromal component of 43 primary tumors and matched lymph node samples, respectively. Fibroblasts were characterized for their expression of alpha-smooth muscle actin (alpha-SMA) and vimentin. Moreover, we verified the level of these proteins in the stromal compartment from normal adjacent tissue and in non-compromised lymph nodes. Our immunohistochemistry revealed that 59 % of fibroblasts associated with primary tumors and 41 % of the respective metastatic lymph nodes (p = 0.271) displayed positive staining for RhoA. In line with this, 57.1 % of fibroblasts associated with primary tumors presented Rac1-positive staining, and the frequency of co-positivity within the lymph nodes was 42.9 % (p = 0.16). Expression of RhoA and Rac1 was absent in fibroblasts of adjacent normal tissue and in compromised lymph nodes. Based on our findings that no significant changes were observed between primary and metastatic lymph nodes, we suggest that fibroblasts are active participants in the invasion of cancer cells to lymph nodes and support the hypothesis that metastatic tumor cells continue to depend on their microenvironment.
bookPart Protocolos teóricos práticos em biologia molecular aplicada a câncer(2015) MACHADO, Camila Longo; PASINI, Fátima Solange; MANGONE, Flavia Regina Rotea; KATAYAMA, Maria Lucia Hirata; ROELA, Rosimeire Aparecida; MAZZOTTI, Tatiane Ktsue Furuya; CHAMMAS, RogerconferenceObject Frequency of CDH1 germline mutations in diffuse gastric cancer in Brazil.(2015) GUINDALINI, Rodrigo Santa Cruz; PASINI, Fatima Solange; GOUVEA, Ana Carolina Ribeiro Chaves De; BRANAS, Priscila Abduch; CORMEDI, Marina Candido Visontai; MAISTRO, Simone; KATAYAMA, Maria Lucia Hirata; ENCINAS, Giselly; NAGY, Tauana; ESTEVEZ-DIZ, Maria Del Pilar; SAFATLE-RIBEIRO, Adriana Vaz; RIBEIRO, Ulysses; FOLGUEIRA, Maria A. A. KoikeconferenceObject Somatic mutations in Luminal HER2 negative tumors from young breast cancer patients.(2015) ENCINAS, Giselly; ESTEVEZ-DIZ, Maria Del Pilar; LYRA, Eduardo C.; KATAYAMA, Maria Lucia Hirata; PASINI, Fatima Solange; MAISTRO, Simone; SABELNYKOVA, Veronica; BOUTROS, Paul; BRENTANI, Maria Mitzi; BASSO, Ricardo; GOUVEA, Ana Carolina Ribeiro Chaves De; CHAMMAS, Roger; GOES, Joao Carlos Guedes Sampaio; FOLGUEIRA, Maria A. A. Koike