LUISA LINA VILLA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina - Docente
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
53 resultados
Resultados de Busca
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- Distribution and factors associated with salivary secretory leukocyte protease inhibitor concentrations(2016) RAHMAN, S.; CAMPBELL, C. M. Pierce; TORRES, B. N.; O'KEEFE, M. T.; INGLES, D. J.; VILLA, L. L.; SILVA, R. J. Carvalho da; CINTRA, R. C.; LAZCANO-PONCE, E.; SALMERON, J.; QUITERIO, M.; GIULIANO, A. R.ObjectivesThis cross-sectional study examined the distribution and correlates of salivary secretory leukocyte protease inhibitor (SLPI) concentrations within a multinational cohort of men. MethodsExtracellular SLPI was measured in oral gargle cell supernatants of 378 men from three countries using an ELISA-based assay. Risk factor data were collected by a questionnaire. Factors associated with SLPI were assessed using linear and logistic regression for continuous and categorical SLPI, respectively. ResultsAmong men aged 18-73years, the median SLPI concentration was 492.0ngml(-1) (range: 2.3-1919.9). In multivariable modeling, men in Brazil and younger men (18-30years) were more likely to have higher levels of SLPI [adjusted odds ratio (aOR) 3.84; 95% confidence interval (CI): 1.94-7.59, and aOR 3.84; 95% CI: 1.98-7.43, respectively]. Men with a self-reported sexually transmitted diseases diagnosis in the past 6months were more likely to have higher SLPI levels (aOR 2.98; 95% CI: 1.1-7.83) and men reporting bleeding/swollen gums were less likely to have higher SLPI (aOR 0.34; 95% CI: 0.15-0.79). Similar results were observed for linear regression models. ConclusionsSecretory leukocyte protease inhibitor concentrations varied significantly by country and decreased with increasing age. The interaction between SLPI, modifiable factors, and oral infections that influence cancer risk warrants further investigation.
- Oral HPV prevalence assessment by Linear Array vs. SPF10 PCR-DEIA-LiPA(25) system in the HPV Infection in Men (HIM) study(2020) BETTAMPADI, Deepti; SIRAK, Bradley A.; FULP, William J.; ABRAHAMSEN, Martha; VILLA, Luisa L.; LAZCANO-PONCE, Eduardo; SALMERON, Jorge; ISAACS-SORIANO, Kimberly A.; BAGGIO, Maria L.; TRENADO, Manuel Quiterio; GIULIANO, Anna R.Introduction: Oral human papillomavirus (HPV) attributable oropharyngeal cancers are on the rise in many countries. Oral HPV infections among healthy individuals are commonly detected using oral gargle samples. However, the optimal method for HPV genotyping oral gargle specimens in research studies has not been previously evaluated. Materials and methods: Oral gargle samples from 1455 HPV Infection in Men (HIM) study participants were HPV genotyped using two different methods: Linear Array and the SPF10 PCR-DEIA-LiPA(25). The sensitivity of the two tests for detecting individual HPV types and grouped HPV types, high-risk HPV, low-risk HPV, grouped 4-HPV-vaccine types, and grouped 9-HPV-vaccine-types, and the degree of concordance between the two tests was assessed. We also examined whether socio-demographic-behavioral factors were associated with concordance between the two assays. Results: The sensitivity of SPF10 PCR-DEIA-LiPA(25) was higher than Linear Array, with the exception of HPV 70, for the detection of oral HPV. The prevalence ratio of SPF10 PCR-DEIA-LiPA(25) to Linear Array varied between 1.0 and 9.0 for individual HPV genotypes, excluding HPV 70, and between 3.8 and 4.4 for grouped 4-valent and 9-valent HPV vaccine types, respectively. There was no association between socio-demographic-behavioral factors and discordance in results between the two tests for oral HPV 16 detection. Discussion: SPF10 PCR-DEIA-LiPA(25) was more sensitive than Linear Array for detecting HPV in oral gargle samples. Given the growing importance of detecting oral HPV infection for research studies of oral HPV natural history and vaccine effectiveness evaluation, we recommend using methods with higher sensitivity such as SPF10 PCR-DEIA-LiPA(25) for detecting HPV in oral gargle samples.
conferenceObject Co-infection with Epstein-Barr virus impacts oral HPV persistence(2022) DICKEY, Brittney L.; GIULIANO, Anna R.; SIRAK, Bradley; ABRAHAMSEN, Martha; LAZCANO-PONCE, Eduardo; VILLA, Luisa L.; COGHILL, Anna E.- THE AGE-SPECIFIC DISTRIBUTION OF GENITAL HUMAN PAPILLOMAVIRUS (HPV) INFECTION AND HERPES SIMPLEX VIRUS-2 (HSV-2) ANTIBODIES AMONG MEN WITH GENDER-FIXED AND GENDER-FLUID SEXUAL BEHAVIOUR: THE HIM STUDY(2013) NYITRAY, A. G.; ROSS, M. W.; WILKERSON, M.; VILLA, L. L.; ABRAHAMSEN, M.; PAPENFUSS, M.; LAZCANO-PONCE, E.; GIULIANO, A. R.
- Human Papillomavirus Prevalence Among 88 Male Virgins Residing in Brazil, Mexico, and the United States(2016) LIU, Zhiyue; NYITRAY, Alan G.; HWANG, Lu-Yu; SWARTZ, Michael D.; ABRAHAMSEN, Martha; LAZCANO-PONCE, Eduardo; SALMERN, Jorge; QUITERIO, Manuel; VILLA, Luisa L.; BAGGIO, Maria Luiza; SILVA, Roberto J. C.; GIULIANO, Anna R.This study determined the prevalence and risk factors for genital human papillomavirus (HPV) detection among men who deny ever engaging in penetrative sex. A questionnaire was administered to 4123 men from a cohort study of HPV natural history. Genital exfoliated cells were collected and genotyped for 36 HPV types. Eighty-eight men were classified as virgins. Log-binomial regression models identified factors associated with genital HPV detection. The prevalence of any and high-risk HPV types among 88 male virgins was 25.0% and 18.2%, respectively. Age and smoking status were associated with HPV detection. Further studies are needed to better understand the risk for HPV infection among male virgins.
- Planning cancer control in Latin America and the Caribbean(2013) GOSS, Paul E.; LEE, Brittany L.; BADOVINAC-CRNJEVIC, Tanja; STRASSER-WEIPPL, Kathrin; CHAVARRI-GUERRA, Yanin; LOUIS, Jessica St; VILLARREAL-GARZA, Cynthia; UNGER-SALDANA, Karla; FERREYRA, Mayra; DEBIASI, Marcio; LIEDKE, Pedro E. R.; TOUYA, Diego; WERUTSKY, Gustavo; HIGGINS, Michaela; FAN, Lei; VASCONCELOS, Claudia; CAZAP, Eduardo; VALLEJOS, Carlos; MOHAR, Alejandro; KNAUL, Felicia; ARREOLA, Hector; BATURA, Rekha; LUCIANI, Silvana; SULLIVAN, Richard; FINKELSTEIN, Dianne; SIMON, Sergio; BARRIOS, Carlos; KIGHTLINGER, Rebecca; GELRUD, Andres; BYCHKOVSKY, Vladimir; LOPES, Gilberto; STEFANI, Stephen; BLAYA, Marcelo; SOUZA, Fabiano Hahn; SANTOS, Franklin Santana; KAEMMERER, Alberto; AZAMBUJA, Evandro de; ZORILLA, Andres Felipe Cardona; MURILLO, Raul; JERONIMO, Jose; TSU, Vivien; CARVALHO, Andre; GIL, Carlos Ferreira; STERNBERG, Cinthya; DUENAS-GONZALEZ, Alfonso; SGROI, Dennis; CUELLO, Mauricio; FRESCO, Rodrigo; REIS, Rui Manuel; MASERA, Guiseppe; GABUS, Raul; RIBEIRO, Raul; KNUST, Renata; ISMAEL, Gustavo; ROSENBLATT, Eduardo; ROTH, Berta; VILLA, Luisa; SOLARES, Argelia Lara; LEON, Marta Ximena; TORRES-VIGIL, Isabel; COVARRUBIAS-GOMEZ, Alfredo; HERNANDEZ, Andres; BERTOLINO, Mariela; SCHWARTSMANN, Gilberto; SANTILLANA, Sergio; ESTEVA, Francisco; FEIN, Luis; MANO, Max; GOMEZ, Henry; HURLBERT, Marc; DURSTINE, Alessandra; AZENHA, GustavoNon-communicable diseases, including cancer, are overtaking infectious disease as the leading health-care threat in middle-income and low-income countries. Latin American and Caribbean countries are struggling to respond to increasing morbidity and death from advanced disease. Health ministries and health-care systems in these countries face many challenges caring for patients with advanced cancer: inadequate funding; inequitable distribution of resources and services; inadequate numbers, training, and distribution of health-care personnel and equipment; lack of adequate care for many populations based on socioeconomic, geographic, ethnic, and other factors; and current systems geared toward the needs of wealthy, urban minorities at a cost to the entire population. This burgeoning cancer problem threatens to cause widespread suffering and economic peril to the countries of Latin America. Prompt and deliberate actions must be taken to avoid this scenario. Increasing efforts towards prevention of cancer and avoidance of advanced, stage IV disease will reduce suffering and mortality and will make overall cancer care more affordable. We hope the findings of our Commission and our recommendations will inspire Latin American stakeholders to redouble their efforts to address this increasing cancer burden and to prevent it from worsening and threatening their societies.
- Cutaneous human papillomayirus types detected on the surface of male external genital lesions: A case series within the HPV Infection in Men Study(2013) CAMPBELL, Christine M. Pierce; MESSINA, Jane L.; STOLER, Mark H.; JUKIC, Drazen M.; TOMMASINO, Massimo; GHEIT, Tarik; ROLLISON, Dana E.; SICHERO, Laura; SIRAK, Bradley A.; INGLES, Donna J.; ABRAHAMSEN, Martha; LU, Beibei; VILLA, Luisa L.; LAZCANO-PONCE, Eduardo; GIULIANO, Anna R.Background: Cutaneous human papillomaviruses (HPVs) may be associated with cutaneous epithelial lesions and non-melanoma skin cancers. No study has systematically evaluated the presence of genus beta []-HPV in male genital skin or external genital lesions (EGLs) Objectives: To examine cutaneous beta-HPV types detected on the surface of EGLs in men and describe their presence prior to EGL development. Study design: A retrospective case series was conducted among 69 men with pathologically confirmed EGLs (n=72) who participated in the HPV Infection in Men Study. Archived exfoliated cells collected from the surface of each EGL and normal genital skin specimens 6-12 months preceding EGL development were tested for beta-HPV DNA using a type-specific multiplex genotyping assay. Results: beta-HPV DNA was detected on 61.1% of all EGLs, with types 38(16.7%), 5(15.3%), and 12(12.5%) most commonly identified. HPV prevalence differed across pathological diagnoses, with the largest number of beta-HPV types detected on condylomas. Most beta-HPV types were detected on normal genital skin prior to EGL development, though the prevalence was lower on EGLs compared to preceding normal genital skin. Conclusions: EGLs and the normal genital skin of men harbor a large number of beta-HPV types; however, it appears that beta-HPVs are unrelated to EGL development in men. Despite evidence to support a causal role in skin carcinogenesis at UVR-exposed sites, cutaneous HPV appears unlikely to cause disease at the UVR-unexposed genitals.
- The Association between Smoking and Anal Human Papillomavirus in the HPV Infection in Men Study(2022) UMUTONI, Victoria; SCHABATH, Matthew B.; NYITRAY, Alan G.; WILKIN, Timothy J.; VILLA, Luisa L.; LAZCANO-PONCE, Eduardo; GIULIANO, Anna R.; SUDENGA, Staci L.Background: Previous studies show an association between smoking and anal cancer. The objective of this study was to assess the association between smoking and anal HPV (human papillo-mavirus) prevalence, incidence, and persistence in men. Methods: The HPV Infection in Men (HIM) Study is a multinational study that enrolled HIV-negative men. At baseline and follow-up visits, anal specimens were collected. HPV geno-typing was assessed by linear array. Prevalence ratios (PR) were used to assess the association between smoking and anal HPV prevalence. Odds ratios (OR) were used to assess the association between smoking and anal HPV incidence and >= 12-months persistence. Results: Current smokers have a higher prevalence [adjusted PR (aPR), 1.36; 95% confidence interval (CI), 1.06-1.73) and incidence [adjusted OR (aOR), 1.74; 95% CI, 1.26-2.39] and >= 12-months persistence (aOR, 1.67; 95% CI, 1.19-2.33) of any anal HPV compared with never smokers. There were no differences in the prevalence, incidence, or persistence of anal HPV between former and never smokers. Smoking status was not associated with the prevalence or persistence of anal HPV among men who have sex with men but was associated with higher incidence of HR-HPV. Among men that have sex with women (MSW), current smokers had an increased prevalence and incidence of LR-HPV compared with never smokers. Conclusions: Current smokers had a higher prevalence, persistence, and incidence of HPV compared with never smokers. Further research is needed to assess the role smoking in anal HPV persis-tence and progression to disease. Impact: Prevention initiatives should raise awareness about smoking and the risk factor of anal HPV infection and anal cancer.
conferenceObject Logistical issues in implementing a clinical trial on oral cancer prevention through HPV vaccination: Implementation of Ulacnet201 in Mexico(2022) ALLEN-LEIGH, Betania; PORTILLO-ROMERO, Alejandra; QUITERIO, Manuel; ACOSTA, Maribel; RIVERA-RAMIREZ, Abraham; SANCHEZ, Guillermina; CRUZ, Aurelio; BARRIENTOS, Tonatiuh; MAGIS, Carlos; ISAACS-SORIANO, Kimberly; ABRAHAMSEN, Martha E.; HOUSE, Margaret; BROFSKY, Emma; SAHASRABUDDHE, Vikrant; WILKIN, Timothy; GIULIANO, Anna; VILLA, Luisa; LAZCANO-PONCE, Eduardo- Cutaneous beta human papillomaviruses and the development of male external genital lesions: A case-control study nested within the HIM Study(2016) CAMPBELL, Christine M. Pierce; GHEIT, Tarik; TOMMASINO, Massimo; LIN, Hui-Yi; TORRES, B. Nelson; MESSINA, Jane L.; STOLER, Mark H.; ROLLISON, Dana E.; SIRAK, Bradley A.; ABRAHAMSEN, Martha; SILVA, Roberto J. Carvalho da; SICHERO, Laura; VILLA, Luisa L.; LAZCANO-PONCE, Eduardo; GIULIANO, Anna R.Background: Cutaneous human papillomaviruses (HPVs) increase the risk of non-melanoma skin cancer in sun-exposed skin. We examined the role of beta-HPV in the development of male external genital lesions (EGLs), a sun-unexposed site. Methods: In this nested case-control study (67 men with pathologically-confirmed EGLs and 134 controls), exfoliated cells collected from the surface of lesions and normal genital skin 0, 6, and 12 months preceding EGL development were tested for beta-HPV DNA using a type-specific multiplex genotyping assay. Beta-HPV prevalence was estimated and conditional logistic regression was used to evaluate the association with condyloma, the most common EGL. Results: While beta-HPV prevalence among controls remained stable, the prevalence among cases was lowest on the surface of lesion. Detecting beta-HPV on the normal genital skin was not associated with the presence or development of condyloma. Conclusions: Cutaneous beta-HPV does not appear to be contributing to pathogenesis in male genital skin. (C) 2016 Published by Elsevier Inc.